HLA from Benchtop to Bedside provides the reader with a comprehensive, concise and thoroughly up-to-date book on all aspects of the HLA system, including new techniques and methodologies. Each chapter begins with bullet point lists of principle learning points, including comprehensive references and validated links to international resources. Written by a diverse range of international academics for professionals, researchers, undergraduate and graduate students, this book is ideal for organ and stem cell transplant professionals, histocompatibility laboratory professionals and staff, medical residents and fellows on transplant services, medical students, and students in clinical laboratory science.
The book's author, Dr. Arthur Bradley Eisenbrey, is an experienced transplant pathologist who has held significant academic and leadership positions in the field.
Author(s): A. Bradley Eisenbrey
Publisher: Academic Press
Year: 2021
Language: English
Pages: 156
City: London
Front-Matter_2021_HLA-from-Benchtop-to-Bedside
HLA from Benchtop to Bedside
Copyright_2021_HLA-from-Benchtop-to-Bedside
Copyright
Preface_2021_HLA-from-Benchtop-to-Bedside
Preface
Acknowledgments_2021_HLA-from-Benchtop-to-Bedside
Acknowledgments
Chapter-1---Introduction-and-some-history_2021_HLA-from-Benchtop-to-Bedside
1 . Introduction and some history
Fundamentals: innate and adaptive immunity
References
Chapter-2---The-structure-of-the-major-histocompatibil_2021_HLA-from-Benchto
2 . The structure of the major histocompatibility complex and major HLA components
HLA class I
Function of HLA Class Ia molecules
HLA Ib molecules
Other HLA class I histocompatibility genes: MICA and MICB
HLA class II
References
Chapter-3---Nomenclature-and-naming-conventions_2021_HLA-from-Benchtop-to-Be
3 . Nomenclature and naming conventions for HLA
HLA allele naming
References
Chapter-4---The-crossmatch_2021_HLA-from-Benchtop-to-Bedside
4 . The crossmatch
Microcytotoxicity and the ``NIH standard'' technique
Antihuman globulin
Crossmatching by microcytotoxicity
ABO and solid organ transplantation
T and B cell isolation
Immunofluorescence
Flow cytometry
HLA antibody detection in tissues
References
Further reading
Chapter-5---HLA-antibody-detection--screening--an_2021_HLA-from-Benchtop-to-
5 . HLA antibody detection (screening) and identification
Antibody identification by microlymphocytotoxicity
Interpretation of antibody specificity in cell-based assays
Panel reactive antibody
Solid-phase antibody screening and identification
Commercial solid-phase HLA antibody assays are qualitative, not quantitative
Clinical indications for HLA antibody testing
Single antigen bead result interpretation in clinical practice
MFI as a quantitative result
Virtual crossmatch
Management of antibody-mediated transplant rejection
HLA in Transfusion Medicine and Blood Banking
TRALI
Platelet transfusion support
References
Chapter-6---HLA-typing-by-serologic-methods_2021_HLA-from-Benchtop-to-Bedsid
6 . HLA typing by serologic methods
References
Chapter-7---HLA-typing-by-molecular-methods_2021_HLA-from-Benchtop-to-Bedsid
7 . HLA typing by molecular methods
Molecular HLA typing ``before PCR''
Sequencing
Oligonucleotide probing and endonuclease cutting
Restriction fragment length polymorphism
Polymerase chain reaction and the molecular revolution in clinical medicine
Polymerase chain reaction sequence specific primers
PCR-RFLP
PCR-rSSO
High resolution typing ambiguities in molecular HLA typing
The challenge of null alleles
rtPCR (aka qPCR, real-time PCR, and quantitative PCR)
References
Chapter-8---Massively-parallel--next-generation-_2021_HLA-from-Benchtop-to-B
8 . Massively parallel (next generation) sequencing
NGS sequencing by Hybridization and Ligation
Selection of molecular HLA typing methods
References
Chapter-9---HLA-alleles--disease-associations--and-_2021_HLA-from-Benchtop-t
9 . HLA alleles, disease associations, and HLA pharmacogenetics
The mathematics of disease associations
How do HLA alleles cause disease?
References
Chapter-10---Engraftment-monitoring_2021_HLA-from-Benchtop-to-Bedside
10 . Engraftment monitoring
A little history
Microsatellite DNA, VNTRs, STRs
Engraftment monitoring
Engraftment monitoring by capillary electrophoresis
Alternative platforms for engraftment monitoring
References
Further reading
Index_2021_HLA-from-Benchtop-to-Bedside
Index
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