High Pressure Effects in Molecular Biophysics and Enzymology is designed to acquaint biochemists, biophysicists, and graduate students with advances in the application of high pressure in connection with spectroscopy as a research tool in the study of biomolecules. The 23 chapters written by leading authorities present an overview of current approaches to the use of high pressure in research on enzyme kinetics, protein folding and structure, lipid bilayer structure and organization, lipid-protein interaction, and DNA structure. This important, timely volume is the first devoted exclusively to high-pressure effects in biochemistry and will be the definitive reference in its subject for the next several years.
Author(s): John L. Markley, Dexter B. Northrop, Catherine A. Royer
Year: 1996
Language: English
Pages: 400
Contents......Page 8
Contributors......Page 11
1. Resolution of the Ambiguity of van't Hoff Plots by the Effect of Pressure on the Equilibrium......Page 18
2. Pressure-Tuning Spectroscopy: A Tool for Investigating Molecular Interactions......Page 29
3. Use of Partial Molar Volumes of Model Compounds in the Interpretation of High-Pressure Effects, on Proteins......Page 48
4. Pressure-Tuning Spectroscopy of Proteins: Fourier Transform Infrared Studies in the Diamond Anvil Cell......Page 59
5. Temperature- and Pressure-Induced Unfolding of a Mutant of Staphylococcal Nuclease A......Page 77
6. Pressure-Jump Relaxation Kinetics of Unfolding and Refolding Transitions of Staphylococcal Nuclease and Proline Isomerization Mutants......Page 89
7. High-Pressure FTIR Studies of the Secondary Structure of Proteins......Page 102
8. High-Pressure NMR Studies of the Dissociation of Arc Repressor and the Cold Denaturation of Ribonuclease A......Page 111
9. Exploring Structural, Functional, and Kinetic Aspects of Nucleic Acid-Protein Complexes with Pressure: Nucleosomes and RNA Polymerase......Page 125
10. Pressure and Cold Denaturation of Proteins, Protein-DNA Complexes, and Viruses......Page 148
11. Sequence, Salt, Charge, and the Stability of DNA at High Pressure......Page 164
12. Application of Pressure Relaxation to the Study of Substrate Binding to Cytochrome P-450[sub(CAM)] versus Temperature, Pressure, and Viscosity......Page 186
13. Pressure Effects on the Ligand-Binding Kinetics for Hemoproteins and Their Site-Directed Mutants......Page 196
14. Transient Enzyme Kinetics at High Pressure......Page 211
15. Steady-State Enzyme Kinetics at High Pressure......Page 226
16. Effects of High Pressure on the Allosteric Properties of Phosphofructokinase from Escherichia coli......Page 257
17. Correlation Field Splitting of Chain Vibrations: Structure and Dynamics in Lipid Bilayers and Biomembranes......Page 271
18. High-Pressure Effects on the Structure and Phase Behavior of Model Membrane Systems......Page 289
19. Membrane-Free Volume Variation with Bulky Lipid Concentration by Regular Distribution: A Functionally Important Membrane Property Explored by Pressure Studies of Phosphatidylcholine Bilayers......Page 313
20. NMR Studies of the Order and Dynamics of Dipalmitoylphosphatidylcholine Bilayers as a Function of Pressure......Page 329
21. Effects of Increased Viscosity on the Function of Integral Membrane Proteins......Page 346
22. Pressure- and Temperature-Induced Inactivation of Microorganisms......Page 361
23. Osmotic and Hydrostatic Pressure as Tools to Study Molecular Recognition......Page 379
B......Page 390
D......Page 391
H......Page 392
M......Page 393
P......Page 394
S......Page 395
X......Page 396
