This book describes the complex structures of heparins and heparan sulfates (heparinoids) and how they are generated by their biosynthetic pathways. The book also details the methodologies for studying these structures and their cellular metabolism. Heparin-Binding Proteins introduces the general nature of interactions between heparinoids and proteins, and presents the role for these structures in their interactions with the proteins of the hemostatic mechanisms, fibroblasts growth factors, superoxide dismutase, and lipoproteins. Key Features * Covers cellular metabolism of heparinoid proteoglycans * Written by a distinguished expert in the field of carbohydrate biochemistry * Describes the roles of heparan sulfate proteoglycans in * * Blood coagulation and fibrinolysis * Lipoprotein metabolism * Superoxide dismutase activity * Fibroblast growth factor responses of cells
Author(s): H. Edward Conrad
Edition: 1st
Publisher: Academic Press
Year: 1997
Language: English
Pages: 553
Front Cover......Page 1
Heparin-Binding Proteins......Page 4
Copyright Page......Page 5
Table of Contents......Page 6
Preface......Page 14
Conventions, Abbreviation, and Terminology......Page 16
II. Heparinoids Defined......Page 22
IV. Heparin-Binding Proteins......Page 24
V. Heparin Binding to Antithrombin: the Protototype for Heparin-Binding Proteins......Page 25
VI. Theme......Page 26
I. The Composition of Heparinoids......Page 28
Il. Heparin and Heparaa Sulfate Structures......Page 31
III. Biosynthesis of Heparinoids: What it Teaches about Structures......Page 43
IV. The Size and Variability of Heparinoids......Page 68
V. The Shape of Heparinoids......Page 69
VI. Proteoglycan Forms of Heparinoids......Page 73
Additional References......Page 81
I. Introduction......Page 82
II. Acid Hydrolysis and Monosaccharide Analysis......Page 84
Ill. Cleavage of Heparin with Retention of Sulfate Substituents......Page 89
IV. Determination of Oligosacchadde Structures......Page 119
V. Determination of Molecular Weight......Page 125
VI Physical Methods in Heparinoid Structural Studies......Page 129
VII Use of Antibodies......Page 134
Additional References......Page 135
I. Modification of Polymeric Heparinoids......Page 136
II. Modified Heparinoids in Structural and Biological Studies......Page 148
llI. Synthesis of Oligosaccharides......Page 156
Additional References......Page 157
I. Overview......Page 158
II. Methods for Studying Proteoglycan Metabolism......Page 160
III. Metabolism of Heparan Sulfate Proteoglycans......Page 169
IV. Perturbation of Heparinoid Metabolism......Page 182
V. Metabolism of Exogenous Heparinoids......Page 190
VI. Dynamic Aspects of HSPG Metabolism--a Key to its Function?......Page 193
Additional References......Page 202
I. Aspects of Heparinoid/Protein Interactions......Page 204
lI. Heparinoids and Proteins. Partners in Binding......Page 206
IlI. The Binding of Metal Ions by Heparinoids......Page 217
IV. Methodology......Page 218
Additional References......Page 223
I. The Heparin/Antithrombin Interaction......Page 224
II. The Role of Antithrombin in Vivo......Page 254
Ill. Antithrombin and Thrombin as Prototypes for Heparin-Binding Proteins......Page 257
Additional References......Page 258
I. Hemostasis......Page 260
II. Interactions of Heparinoids with the Heparin-Binding Proteins in Hemostasis......Page 272
IlI. Summary......Page 314
Additional References......Page 319
I. Introduction......Page 322
II. FGF Structures and Properties......Page 324
IlI. Metabolism of FGFs......Page 328
IV. Interaction of Heparinoids with FGFs and their Receptors......Page 337
V. Involvement of lteparinoids in FGF Metabolism: Caveats......Page 363
VI. Summary......Page 368
Additional References......Page 369
I. Superoxide Generation during the Inflammation Process......Page 372
II. Superoxide Dismutase Isozymes......Page 373
III. The Structure of EC-SOD......Page 374
IV. Metabolism of EC-SOD......Page 379
V .HSPG in the Regulation of EC-SOD Activity......Page 382
Additional References......Page 387
I. Overview......Page 388
II. Lipases......Page 392
IlI. Heparin Binding Lipases......Page 396
IV. The Role of HSPG in the Endocytosis of Lipoproteins......Page 416
Additional References......Page 432
II. Roles of HSPG......Page 434
III. HSPG Metabolism......Page 437
IV. Heparin-Binding Proteins......Page 438
Appendix......Page 442
References......Page 444
Index......Page 536
Color Plate Section......Page 550
