It is generally recognized that the commercial success of biotechnology products is highly dependent on the successful development and application of high-powered separation and purification methods. In this practical and authoritative handbook, the separation of proteins, nucleic acids, and oligonucleotides from biological matrices is covered from analytical to process scales. Also included in a chapter on the separation of monoclonal antibodies, which have found numerous uses as therapeutic and diagnostic agents. Analytical techniques include an interesting montage of chromatographic methods, capillary electrophoresis, isoelectric focusing, and mass spectrometry. Among separation and purification methods, liquid-liquid distribution, displacement chromatography, expanded bed adsorption, membrane chromatography, and simulated moving bed chromatography are covered at length. Regulatory and economic considerations are addressed, as are plant and process equipment and engineering process control. A chapter on future developments highlights the application of DNA chip arrays as well as evolving methodologies for a large number of drugs that are under development for treatment of cancer, AIDS, rheumatoid arthritis, and Alzheimer's disease. Handbook of Bioseparations serves as an essential reference and guidebook for separation scientists working in the pharmaceutical and biotechnology industries, academia, and government laboratories. Key Features* Covers bioseparations of proteins, nucleic acids, and monoclonal antibodies* Encompasses both analytical and process-scale methods* Elucidates the importance of engineering process control* Details selection of plant and process equipment* Addresses economic considerations* Discusses future developments
Author(s): Satinder Ahuja (Eds.)
Series: Separation Science and Technology 2
Edition: 1
Publisher: Academic Press
Year: 2000
Language: English
Pages: 1-722
Content:
Editorial Board
Page v
Contributors
Pages xi-xii
Preface
Pages xiii-xiv
Satinder Ahuja
Bioseparations: An overview Original Research Article
Pages 1-21
S. Ahuja
Analysis of protein impurities in pharmaceuticals derived from recombinant dna Original Research Article
Pages 23-70
Donald O. O'Keefe
Physicochemical factors in polypeptide and protein purification and analysis by high-performance liquid chromatographic techniques: current status and challenges for the future Original Research Article
Pages 71-235
Milton T.W. Hearn
Capillary electrophoresis of compounds of biological interest Original Research Article
Pages 237-262
S. Ahuja
Isoelectric focusing Original Research Article
Pages 263-298
David E. Garfin
Mass spectrometry of biomolecules Original Research Article
Pages 299-327
Dan Gibson, Catherine E. Costello
Liquid-liquid partitioning methods for bioseparations Original Research Article
Pages 329-364
Tingyue Gu
Separation of nucleic acids and proteins Original Research Article
Pages 365-378
Rohit Harve, Rakesh Bajpai
9 Bioseparations by displacement chromatography Original Research Article
Pages 379-415
Steven M. Cramer
10 Physicochemical basis of expanded-bed adsorption for protein purification Original Research Article
Pages 417-430
B. Mattiasson, M.P. Nandakumar
Expanded-bed adsorption process for protein capture Original Research Article
Pages 431-451
Joseph Shiloach, Robert M. Kennedy
Adsorptive membranes for bioseparations Original Research Article
Pages 453-474
Ranjit R. Deshmukh, Timothy N. Warner
Simulated moving-bed chromatography for biomolecules Original Research Article
Pages 475-509
R. M. Nicoud
Large-Scale chromatographic purification of ougonucleotides Original Research Article
Pages 511-534
Ranjit R. Deshmukh, William E. Leitch II, Yogesh S. Sanghvi, Douglas L. Cole
15 Separation of antibodies by liquid chromatography Original Research Article
Pages 535-632
Egisto Boschetti, Alois Jungbauer
Processing plants and equipment Original Research Article
Pages 633-657
P. Bowles
Engineering process control of bioseparation processes Original Research Article
Pages 659-665
Randel M. Price, Ajit Sadana
Economics of bioseparation processes Original Research Article
Pages 667-685
Anand Ramakrishnan, Ajit Sadana
Future developments Original Research Article
Pages 687-711
S. Ahuja
Index
Pages 713-722
