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Guide to Molecular Cloning Techniques

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This self-contained, state-of-the-art manual is designed to meet the needs of the student, experienced researcher, and newcomer to the molecular biology discipline seeking an efficient means of obtaining a clone. Available in an easy-to-use spiral-bound edition, Guide to Molecular Cloning Techniques progresses from basic techniques underlying much of recombinant DNA technology to a series of sections addressing commonly met problems. Key Features * Comprehensive protocols compiled specifically for this volume * Among the topics covered: * Genomic cloning * Preparation and characterization of mRNA cDNA cloning * Screening libraries * Identification and characterization of clones * Methods written by 87 experts * Editors' notes, overviews, and extensive cross-referencing * Process Guide, a compendium of basic processes in molecular biology indexed for at-a-glance accessibility * A problem-solving quantitative approach * Emphasis on strategies for choosing alternative methods

Author(s): Shelby L. Berger, Alan R. Kimmel
Series: Methods in Enzymology 152
Publisher: Academic Press
Year: 1987

Language: English
Pages: 812