Gel Electrophoresis of Proteins: A Practical Approach (Practical Approach Series)

This new edition is almost a completely new text, with eight of the ten chapters written by new authors. It presents the most reliable methods for essential procedures such as one-dimensional polyacrylamide gel electrophoresis, isoelectric focusing, two-dimensional gel electrophoresis, preparative gel electrophoresis, and peptide mapping, complete with the latest refinements of the procedures. In addition, it describes major new techniques developed since the previous edition. These include capillary gel electrophoresis, sequence analysis of gel-resolved proteins, fluorophore-labelled saccharide electrophoresis, and analysis of protein-protein interactions by gel electrophoresis. Like the previous editions, this volume is easy-to-follow and thorough, a laboratory manual written by experienced researchers for researchers. The emphasis is on describing the best methods, in step-by-step detail, with copious advice to ensure that each method works the first time. This new edition is a 'must' for anyone currently uses gel electrophoresis.