G-Protein Coupled Receptors (GPCRs) are not only the largest protein family in the human genome but are also the single biggest target for therapeutic agents. Research into GPCRs is therefore growing at a fast pace and the range of techniques that can be applied to GPCRs is vast and continues to grow. This book provides an invaluable bench-side guide into the best and most up-to-date techniques for current and future research on GPCRs.With contributions from leading international authorities, this book equips readers with clear and detailed protocols for both well-known and up-and-coming techniques along with hints and tips for success. All the methods have been tried and tested by leading international research labs and are presented in easy-to-follow stages along with a useful overview of each technique.This book is an essential resource for all researchers in molecular biology, biochemistry, pharmacology and for graduate students.
Author(s): David Poyner, Mark Wheatley
Edition: 1
Year: 2010
Language: English
Pages: 310
G Protein-Coupled Receptors......Page 4
Contents......Page 8
Preface......Page 12
Contributors......Page 14
1.1 Introduction......Page 18
1.2 Methods and approaches......Page 22
References......Page 44
2.1 Introduction......Page 48
2.2 Methods and approaches......Page 50
2.3 Troubleshooting......Page 66
References......Page 67
3.1 Introduction......Page 70
3.2 Methods and approaches......Page 71
3.3 Troubleshooting......Page 83
References......Page 84
4.1 Introduction......Page 86
4.2 Methods and approaches......Page 87
4.3 Troubleshooting......Page 97
References......Page 99
5.1 Introduction......Page 102
5.2 Methods and approaches......Page 103
5.3 Troubleshooting......Page 123
References......Page 124
6.1 Introduction......Page 128
6.2 Methods and approaches......Page 131
References......Page 145
7.1 Introduction......Page 150
7.2 Methods and approaches......Page 153
7.3 Troubleshooting......Page 160
References......Page 161
8.1 Introduction......Page 164
8.2 Methods and approaches......Page 166
8.3 General considerations, caveats and troubleshooting......Page 179
Acknowledgements......Page 183
References......Page 181
9.1 Introduction......Page 186
9.2 Methods and approaches......Page 187
9.3 Troubleshooting......Page 207
References......Page 208
10.1 Introduction......Page 214
10.2 Methods......Page 215
References......Page 230
11.1 Introduction......Page 232
11.2 Methods and approaches......Page 234
11.3 Troubleshooting......Page 243
References......Page 244
12.1 Introduction......Page 246
12.2 Methods and approaches......Page 247
12.3 Troubleshooting......Page 264
References......Page 265
13.1 Introduction......Page 268
13.2 Methods and approaches......Page 269
13.4 Automated methods for generating models of GPCRs......Page 286
References......Page 287
A.2 Why mutagenesis?......Page 292
A.4 Conclusion......Page 302
References......Page 303
Index......Page 306
