Fluorescence Molecular Tomography: Principles and Applications is the first book to cover the underlying principles and practical applications of fluorescence molecular tomography (FMT) in a systematic manner. Using a tutorial approach, the text begins with an overview of the fundamentals of FMT and goes on to detail image reconstruction approaches (including linear and nonlinear reconstruction algorithms), FMT instrumentations (including time-domain, frequency-domain, and continuous-wave domain systems), and implementation of image-enhancing schemes (including both software and hardware approaches). Further chapters examine multimodal approaches combining photoacoustic tomography (PAT), computed tomography (CT), single-photon emission tomography (SPECT), and magnetic resonance imaging (MRI) and discuss bioluminescence tomography and miniaturized FMT from hand-held to endoscopic FMT. A final chapter looks at clinical applications and animal studies. This authoritative and practical guide will serve as a valuable reference for researchers, scientists, clinicians, and industry professionals.
The first book dedicated to fluorescence molecular tomography (FMT);
Covers underlying principles and practical applications;
Written by a leading FMT research pioneer and expert.
Author(s): Huabei Jiang
Publisher: Springer
Year: 2022
Language: English
Pages: 134
City: Cham
Preface
Contents
Chapter 1: Introduction
1.1 Fluorescence Molecular Tomography
1.2 Mathematic Image Reconstruction
1.2.1 Forward Problem: Fluorescent Photon Diffusion/Transport Model
1.2.2 Inverse Problem
1.3 Fluorescent Probes
Chapter 2: Reconstruction Algorithms
2.1 Linear Reconstruction Algorithm
2.2 Nonlinear Reconstruction Algorithm
2.2.1 Algorithm Implementation
2.2.2 Results
2.2.3 Discussion
Chapter 3: Instrumentation
3.1 Time-Domain FMT System
3.2 Frequency-Domain FMT System
3.3 CW-Domain FMT System
3.4 Contact vs. Noncontact Light Detection Scheme
Chapter 4: Image Enhancing Schemes
4.1 Dual Mesh Scheme
4.2 RTE-Based Fluorescence Molecular Tomography
4.3 The Third-Order Diffusion Equations-Based FMT
4.4 DOT-Guided FMT
4.4.1 Methods and Materials
4.4.2 Results and Discussion
4.5 Fluorophore Lifetime Imaging
4.5.1 Methods and Materials
4.5.2 Results and Discussion
4.6 Full Density FMT
4.6.1 Methods
4.6.2 Results and Discussion
Chapter 5: Bioluminescence Tomography
5.1 BLT Reconstruction Algorithm
5.1.1 Simulations
5.1.2 Experiments
5.1.3 Results and Discussion
5.2 DOT-Guided Bioluminescence Tomography
5.2.1 Methods
5.2.2 Results and Discussion
Chapter 6: Multimodal Approaches
6.1 FMT/PAT
6.1.1 Methods
6.1.2 Results and Discussion
6.1.2.1 Comparison of Image Pattern
6.1.2.2 Comparison of Spatial Resolution
6.1.2.3 Comparison of Sensitivity
6.2 FMT/CT
6.3 FMT/Phase-Contrast CT
6.4 FMT/MRI
6.5 FMT/CT/SPECT
Chapter 7: Miniaturized Fluorescence Molecular Tomography
7.1 Handheld FMT
7.1.1 Methods
7.1.2 Results and Discussion
7.1.2.1 Simulations
7.1.2.2 Phantom Experiments
7.1.2.3 Mouse Experiments
7.2 FMT Pen
7.2.1 Methods
7.2.2 Results and Discussion
7.2.2.1 Phantom Experiments
7.2.2.2 Animal Experiments
7.3 Endoscopic FMT
7.3.1 Methods
7.3.2 Results and Discussion
Chapter 8: Animal Studies and Clinical Applications
8.1 Fruit Flies
8.1.1 Pupa with Dye-Containing Microtube
8.1.2 In Vivo Pupa with DsRed Reporter
8.2 Tumor Imaging in Animals
8.2.1 Ovarian Tumor
8.2.1.1 Methods
Cell Lines and HER-2/Neu-Specific Affibody Conjugation to Magnetic Iron Oxide Nanoparticles (IONPs)
Orthotopic Human Ovarian Cancer Xenograft Model
Fluorescence Molecular Tomography Imaging System
Photoacoustic Tomography System
In Vivo Imaging in Animal Tumor Models
Histology Analysis
8.2.1.2 Results and Discussion
8.2.2 Lung Tumor
8.2.2.1 Methods
8.2.2.2 Results and Discussion
8.2.3 Brain Tumor
8.2.3.1 Methods
8.2.3.2 Results and Discussion
8.3 Breast Tumor Imaging in Humans
8.3.1 Methods
8.3.2 Results and Discussion
8.4 Tumor Response to Therapies
8.4.1 Methods
8.4.2 Results and Discussion
8.5 Quantification of Receptor Density
8.5.1 Methods
8.5.2 Results and Discussion
8.6 Bone Remodeling
8.6.1 Methods
8.6.2 Results and Discussion
8.7 Alzheimer´s Disease
8.7.1 Methods
8.7.2 Results and Discussion
8.8 Cardiovascular Disease
8.8.1 Methods
8.8.2 Results and Discussion
Bibliography
Index
