This book describes developments in the field of super-resolution fluorescence microscopy or nanoscopy. In 11 chapters, distinguished scientists and leaders in their respective fields describe different nanoscopy approaches, various labeling technologies, and concrete applications. The topics covered include the principles and applications of the most popular nanoscopy techniques STED and (f)PALM/STORM, along with advances brought about by fluorescent proteins and organic dyes optimized for fluorescence nanoscopy. Furthermore, the photophysics of fluorescent labels is addressed, specifically for improving their photoswitching capabilities. Important applications are also discussed, such as the tracking and counting of molecules to determine acting forces in cells, and quantitative cellular imaging, respectively, as well as the mapping of chemical reaction centers at the nano-scale.
The 2014 Chemistry Nobel Prize® was awarded for the ground-breaking developments of super-resolved fluorescence microscopy. In this book, which was co-edited by one of the prize winners, readers will find the most recent developments in this field.
Author(s): Philip Tinnefeld, Christian Eggeling, Stefan W. Hell (eds.)
Series: Springer Series on Fluorescence 14
Edition: 1
Publisher: Springer-Verlag Berlin Heidelberg
Year: 2015
Language: English
Pages: 335
Tags: Nanochemistry; Biological Microscopy; Biophysics and Biological Physics; Nanotechnology; Spectroscopy/Spectrometry
Front Matter....Pages i-xii
STED Fluorescence Nanoscopy....Pages 3-25
Super-Resolution Imaging Through Stochastic Switching and Localization of Single Molecules: An Overview....Pages 27-64
A Practical Guide to d STORM: Super-Resolution Imaging with Standard Fluorescent Probes....Pages 65-84
Single-Molecule Photocontrol and Nanoscopy....Pages 87-110
Probes for Nanoscopy: Fluorescent Proteins....Pages 111-158
Tailoring Fluorescent Labels for Far-Field Nanoscopy....Pages 159-188
Probes for Nanoscopy: Photoswitchable Fluorophores....Pages 189-213
Far-Field Nanoscopy with Conventional Fluorophores: Photostability, Photophysics, and Transient Binding....Pages 215-242
NASCA Microscopy: Super-Resolution Mapping of Chemical Reaction Centers....Pages 245-261
Counting Molecules: Toward Quantitative Imaging....Pages 263-291
In Vivo Tracking of Single Biomolecules: What Trajectories Tell Us About the Acting Forces....Pages 293-329
Back Matter....Pages 331-335
