N.-Y.: Wiley-Blackwell, 2012. - 352p.Essential Guide to Reading Biomedical Papers: Recognising and Interpreting Best Practice is an indispensable companion to the biomedical literature. This concise, easy-to-follow text gives an insight into core techniques and practices in biomedical research and how, when and why a technique should be used and presented in the literature. Readers are alerted to common failures and misinterpretations that may evade peer review and are equipped with the judgment necessary to be properly critical of the findings claimed by research articles. This unique book will be an invaluable resource for students, technicians and researchers in all areas of biomedicine.
Allows the reader to develop the necessary skills to properly evaluate research articles
Coverage of over 30 commonly-used techniques in the biomedical sciences Global approach and application, with contributions from leading experts in diverse fieldsContent:Philosophy of Science
Ingredients of Experimental Design
Statistics: A Journey that Needs a Guide
Organ Bath Pharmacology
Small Vessel Myography
Mammalian Cell Cultures: The Example of Airway Epithelial Cell Cultures for Cystic Fibrosis Research
Electron Microscopy (TEM and SEM)
Fluorescence Microscopy
Intracellular ‘Sharp’ Microelectrode Recording
Single Electrode Voltage-Clamp (SEVC)
Patch Clamp Recording
Production of Antibodies
Immunocytochemistry and Immunohistochemistry
Immunoprecipitation (IP)
Immunoblotting (Western)
Applications of Green Fluorescent Protein (GFP)
Fluorescent Measurement of Ion Activity in Cells
Detection of Exocytosis – Real Time
Viral Vector Transgenesis
Polymerase Chain Reaction (PCR) and Reverse Transcription (RT)-PCR
In Situ Hybridisation (ISH)
Methods of Interference (Antisense, siRNAs and Dominant Negative Mutations)
Transcriptome Analysis: Microarrays
Experimental Proteomics
Behavioural Methodology
Genetically Modified Mouse Models
Wireless Recording of Cardiovascular Signals
Electrical Stimulation Methods
Extracellular Recording
Antidromic Identification
Event-Triggered Averaging, Including Spike-Triggered Averaging
Axonal Transport Tracing of CNS Pathways
Cardiovascular Methods: General Considerations for Human Studies
Measuring Cardiac Output in Humans
Measuring Peripheral Blood Flow in Humans
