This book covers the fundamental aspects of the electrochemistry and redox enzymes that underlie enzymatic bioelectrocatalysis, in which a redox enzyme reaction is coupled with an electrode reaction. Described here are the basic concept and theoretical aspects of bioelectrocatalysis and the various experimental techniques and materials used to study and characterize related problems. Also included are the various applications of bioelectrocatalysis to bioelectrochemical devices including biosensors, biofuel cells, and bioreactors. This book is a unique source of information in the area of enzymatic bioelectrocatalysis, approaching the subject from a cross-disciplinary point of view.
Author(s): Kenji Kano, Osamu Shirai, Yuki Kitazumi, Kento Sakai, Hong-Qi Xia
Publisher: Springer Singapore
Year: 2021
Language: English
Pages: 135
City: Singapore
Preface
Acknowledgments
Contents
Abbreviations and Symbols
Enzyme
Redox Center
Chemical
Electrode
Others
Symbols
Greek Symbols
1 Redox Proteins and Bioelectrocatalysis
1.1 Oxidoreductases [1, 2]
1.2 Redox Components
1.2.1 NAD(P)(H) [1, 5]
1.2.2 Flavins [1, 6, 7]
1.2.3 Quinones [7, 12]
1.2.4 Hemes [1, 30]
1.2.5 Ion-Sulfur Clusters [1, 7]
1.2.6 Copper [33]
1.2.7 Nickel [39]
1.2.8 Molybdenum [41]
1.2.9 Others
1.3 Bioelectrocatalysis
References
2 MET-Type Bioelectrocatalysis
2.1 Reaction-Layer Approximation in a Homogeneous System
2.2 Selection of Mediators
2.3 Mathematical Model in Immobilized Layers
2.4 Serial Resistance Model for Steady-State Response in MET-Type Bioelectrocatalysis
2.5 Redox Mediators
2.6 Immobilization of Enzymes and Mediators
2.6.1 Bifunctional Reagents
2.6.2 Redox Polymers
2.7 Electrode Materials
References
3 Fundamentals of DET-Type Bioelectrocatalysis
3.1 History of DET-Type Bioelectrocatalysis
3.2 Theory of Steady-State Catalytic Current
3.3 Random Orientation Model of Enzymes
3.4 Data Analysis of the Steady-State DET-Type Bioelectrocatalytic Waves
References
4 Characteristic Properties of Redox Enzymes as Electrocatalysts
4.1 Introduction
4.2 Surface-Area Effect and Curvature Effects (or Cage Effect) on Mesoporous Electrodes
4.3 Interfacial Electrode Kinetics on Microporous Electrodes
4.4 The Control of the Orientation of Adsorbed Enzymes by Surface Modification of Electrodes
4.5 DET-Type Bi-Way Bioelectrocatalysis
References
5 Protein-Engineering Approach for Improvement of DET-Type Bioelectrocatalytic Performance
5.1 Motivation of Protein-Engineering
5.2 Enzyme Trimming
5.3 Site-Directed Mutation
5.4 Protein Surface Modification
References
6 Applications to Biosensors
6.1 Introduction
6.2 Selectivity of Electrochemical Biosensors
6.3 Electrodes for Amperometric Biosensing
6.4 Mass-Transfer-Controlled Amperometric Biosensing
6.5 Potentiometric Coulometry
6.5.1 Bienzyme Biosensing
References
7 Applications to Biofuel Cells and Bioreactors
7.1 From Bioelectrocatalysis to Biofuel Cells and Bioreactors
7.2 Biofuel Cell
7.3 Photobioelectrochemical Water Splitting
7.4 Bioelectrochemical/Biochemical Hydrogen/C1 Economy
References
Conclusions and Outlooks
