This volume covers the preparation and analysis of model systems for biological electron microscopy.This will be the first compendium covering the various aspects of sample preparation of very diverse biological systems. Covers the preparation and analysis of model systems for biological electron microscopy. Includes the most popular systems but also organisms that are less frequently used in cell biology. This issue presents the currently most important methods for the preparation of biological specimens. This will be the first compendium covering the various aspects of sample preparation of very diverse biological systems.
Author(s): Thomas Müller-Reichert (Eds.)
Series: Methods in Cell Biology 96
Edition: 1
Publisher: Academic Press
Year: 2010
Language: English
Pages: 1-724
Tags: Биологические дисциплины;Цитология;
Content:
Series Editors
Page ii
Volume Editors
Page iii
Copyright
Page iv
Contributors
Pages xii-xvii
Preface
Pages xviii-xix
Acknowledgments
Page xx
Chapter 1 - Electron Microscopy of Viruses
Pages 1-20
Michael Laue
Chapter 2 - Bacterial TEM: New Insights from Cryo-Microscopy
Pages 21-45
Martin Pilhofer, Mark S. Ladinsky, Alasdair W. McDowall, Grant J. Jensen
Chapter 3 - Analysis of the Ultrastructure of Archaea by Electron Microscopy
Pages 47-69
Reinhard Rachel, Carolin Meyer, Andreas Klingl, Sonja Gürster, Thomas Heimerl, Nadine Wasserburger, Tillmann Burghardt, Ulf Küper, Annett Bellack, Simone Schopf, Reinhard Wirth, Harald Huber, Gerhard Wanner
Chapter 4 - Chlamydomonas: Cryopreparation Methods for the 3-D Analysis of Cellular Organelles
Pages 71-91
Eileen T. O’Toole
Chapter 5 - Ultrastructure of the Asexual Blood Stages of Plasmodium falciparum
Pages 93-116
Eric Hanssen, Kenneth N. Goldie, Leann Tilley
Chapter 6 - Electron Tomography and Immuno-labeling of Tetrahymena thermophila Basal Bodies
Pages 117-141
Thomas H. Giddings Jr., Janet B. Meehl, Chad G. Pearson, Mark Winey
Chapter 7 - Electron Microscopy of Paramecium (Ciliata)
Pages 143-173
Klaus Hausmann, Richard D. Allen
Chapter 8 - Ultrastructural Investigation Methods for Trypanosoma brucei
Pages 175-196
Johanna L. Höög, Eva Gluenz, Sue Vaughan, Keith Gull
Chapter 9 - Dictyostelium discoideum: A Model System for Ultrastructural Analyses of Cell Motility and Development
Pages 197-216
Michael P. Koonce, Ralph Gräf
Chapter 10 - Toward Sub-second Correlative Light and Electron Microscopy of Saccharomyces cerevisiae
Pages 217-234
Christopher Buser
Chapter 11 - Fission Yeast: A Cellular Model Well Suited for Electron Microscopy Investigations
Pages 235-258
Hélio Roque, Claude Antony
Chapter 12 - Electron Microscopy and High-Pressure Freezing of Arabidopsis
Pages 259-283
Byung-Ho Kang
Chapter 13 - Preparation Techniques for Transmission Electron Microscopy of Hydra
Pages 285-306
Thomas W. Holstein, Michael W. Hess, Willi Salvenmoser
Chapter 14 - Electron Microscopy of Flatworms: Standard and Cryo-Preparation Methods
Pages 307-330
Willi Salvenmoser, Bernhard Egger, Johannes G. Achatz, Peter Ladurner, Michael W. Hess
Chapter 15 - Three-Dimensional Reconstruction Methods for Caenorhabditis elegans Ultrastructure
Pages 331-361
Thomas Müller-Reichert, Joel Mancuso, Ben Lich, Kent McDonald
Chapter 16 - Insects as Model Systems in Cell Biology
Pages 363-394
Thomas A. Keil, R. Alexander Steinbrecht
Chapter 17 - Electron Microscopy of the Amphibian Model Systems Xenopus laevis and Ambystoma mexicanum
Pages 395-423
Thomas Kurth, Jürgen Berger, Michaela Wilsch-Bräuninger, Susanne Kretschmar, Robert Cerny, Heinz Schwarz, Jan Löfberg, Thomas Piendl, Hans H. Epperlein
Chapter 18 - Modern Approaches for Ultrastructural Analysis of the Zebrafish Embryo
Pages 425-442
Nicole L. Schieber, Susan J. Nixon, Richard I. Webb, Viola M.J. Oorschot, Robert G. Parton
Chapter 19 - Transmission Electron Microscopy of Cartilage and Bone
Pages 443-473
Douglas R. Keene, Sara F. Tufa
Chapter 20 - Electron Microscopy of the Mouse Central Nervous System
Pages 475-512
Wiebke Möbius, Benjamin Cooper, Walter A. Kaufmann, Cordelia Imig, Torben Ruhwedel, Nicolas Snaidero, Aiman S. Saab, Frédérique Varoqueaux
Chapter 21 - Rapidly Excised and Cryofixed Rat Tissue
Pages 513-527
Dimitri Vanhecke, Werner Graber, Daniel Studer
Chapter 22 - The Actin Cytoskeleton in Whole Mount Preparations and Sections
Pages 529-564
Guenter P. Resch, Edit Urban, Sonja Jacob
Chapter 23 - Three-Dimensional Cryo-Electron Microscopy on Intermediate Filaments
Pages 565-589
Robert Kirmse, Cédric Bouchet-Marquis, Cynthia Page, Andreas Hoenger
Chapter 24 - Correlative Time-Lapse Imaging and Electron Microscopy to Study Abscission in HeLa Cells
Pages 591-601
Julien Guizetti, Jana Mäntler, Thomas Müller-Reichert, Daniel W. Gerlich
Chapter 25 - Viral Infection of Cells in Culture: Approaches for Electron Microscopy
Pages 603-618
Paul Walther, Li Wang, Sandra Ließem, Giada Frascaroli
Chapter 26 - Intracellular Membrane Traffic at High Resolution
Pages 619-648
Jan R.T. van Weering, Edward Brown, Thomas H. Sharp, Judith Mantell, Peter J. Cullen, Paul Verkade
Chapter 27 - 3D Versus 2D Cell Culture: Implications for Electron Microscopy
Pages 649-670
Michael W. Hess, Kristian Pfaller, Hannes L. Ebner, Beate Beer, Daniel Hekl, Thomas Seppi
Chapter 28 - “Tips and Tricks” for High-Pressure Freezing of Model Systems
Pages 671-693
Kent McDonald, Heinz Schwarz, Thomas Müller-Reichert, Rick Webb, Christopher Buser, Mary Morphew
Subject Index
Pages 695-715
Volumes in Series
Pages 717-724
