2-nd ed. - Humana Press, Totowa NJ.- 2007.- 608 p.
This second edition of Electron Microscopy: Methods and Protocols is written for established researchers as well as new students in the field of
molecular biology. It is not only for biomedical but also for general biological science research and its application. The combination of microscopical and
chemical analyses has provided the basis for our current understanding of cell biology. The high-resolution electron microscope with its associated equipment can serve as a powerful tool for analyzing molecular structure, interactions, and processes.
The second edition now consists of two main areas: the first relates to TEM, and the second covers scanning electron microscopy (SEM) and mass
spectrometry (MS). The TEM area comprises several sections:Conventional and microwave-assisted specimen preparation methods for cultured cells and biomedical and plant tissues, for the benefit of those who only require routine TEM images through ultramicrotomy sectioning and then positive staining.
Cryo-specimen preparation by high-pressure freezing and cryoultramicrotomy.
Negative staining and immunogold labeling techniques for samples prepared through conventional, cryosectioning, or high-pressure freezing methods. Some of these chapters are presented in correlative approaches using TEM with fluorescent or confocal microscopy. Quantitative aspects of immunogold labeling in resin embedded samples are also included.
TEM crystallography and cryo-TEM tomography for the study of membrane proteins, macromolecules, organelles, and cells. One chapter on the application of EELS to biomedical tissue is included.
