The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerlyawaited, frequently consulted, and praised by researchers and reviewers alike. More than 250 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. Key Features* Includes descriptions of functional, structural, kinetic, and genetic methods for analyzing major enzymes of DNA replication* Describes strategies for studying interactions of these proteins during replication* Provides comprehensive descriptions of uses of prokaryotic and eukaryotic crude in vitro replication systems and reconstitution of such systems from purified proteins* Includes methods for analyzing DNA replication in vivo
Author(s): John N. Abelson, Melvin I. Simon, Judith L. Campbell
Series: Methods in Enzymology 262
Edition: 1st
Publisher: Academic Press
Year: 1995
Language: English
Pages: 730
Table of Contents......Page 4
Contributors to Volume 262......Page 8
Preface......Page 12
Section I Purification and Characterization of DNA Polymerases......Page 13
Section II Structural Analysis of DNA Polymerases......Page 157
Section III Mechanisms of DNA Polymerases......Page 200
Section IV Structure-Function Studies of DNA Polymerases......Page 295
Section V Polymerase Accessory Functions. Replication Proteins, Multlenzyme Replication Complexes......Page 401
Sectton VI In Vitro Replication Systems: Crude and Reconstituted......Page 509
Section VII DNA Synthesis in Vivo......Page 599
Author Index......Page 685
Subject Index......Page 713
