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Directed enzyme evolution, screening and selection method

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California Institute of Technology, Pasadena. Text presents experimental protocols for the generation of molecular diversity. Includes reproducible methods for random mutagenesis of genes, for homologous and nonhomologous recombination, and for constructing in vivo libraries in bacteria. For researchers.

Author(s): Frances H. Arnold, George Georgiou
Series: Methods in Molecular Biology Vol 230
Edition: 1
Publisher: Humana Press
Year: 2003

Language: English
Pages: 203