Lipids

Content:
Methods in Cell Biology
Pages i-iii

Copyright
Page iv

Contributors
Pages xi-xiv

Preface
Page xv
Markus R. Wenk, Gilbert Di Paolo

Chapter 1 - Supported Native Plasma Membranes as Platforms for the Reconstitution and Visualization of Endocytic Membrane Budding
Pages 1-18
Min Wu, Pietro De Camilli

Chapter 2 - Studying Lipids Involved in the Endosomal Pathway
Pages 19-46
Christin Bissig, Shem Johnson, Jean Gruenberg

Chapter 3 - Studying In Vitro Membrane Curvature Recognition by Proteins and its Role in Vesicular Trafficking
Pages 47-71
Jean-Baptiste Manneville, Cécile Leduc, Benoit Sorre, Guillaume Drin

Chapter 4 - Reconstituting Multivesicular Body Biogenesis with Purified Components
Pages 73-92
Thomas Wollert

Chapter 5 - Approaches to the Study of Atg8-Mediated Membrane Dynamics In Vitro
Pages 93-116
Anjali Jotwani, Diana N. Richerson, Isabelle Motta, Omar Julca-Zevallos, Thomas J. Melia

Chapter 6 - Reconstitution Assay System for Ceramide Transport With Semi-Intact Cells
Pages 117-129
Keigo Kumagai, Masahiro Nishijima, Kentaro Hanada

Chapter 7 - Visualizing Mitochondrial Lipids and Fusion Events in Mammalian Cells
Pages 131-145
Huiyan Huang, Michael A. Frohman

Chapter 8 - Targeted and Non-Targeted Analysis of Membrane Lipids Using Mass Spectrometry
Pages 147-172
Xue Li Guan, Markus R. Wenk

Chapter 9 - Modulation of Host Phosphoinositide Metabolism During Salmonella Invasion by the Type III Secreted Effector SopB
Pages 173-186
Bernhard Roppenser, Sergio Grinstein, John H. Brumell

Chapter 10 - Acute Manipulation of Phosphoinositide Levels in Cells
Pages 187-207
Belle Chang-Ileto, Samuel G. Frere, Gilbert Di Paolo

Chapter 11 - Regulation of Phosphoinositide-Metabolizing Enzymes by Clathrin Coat Proteins
Pages 209-225
Marnix Wieffer, Volker Haucke, Michael Krauß

Chapter 12 - Phosphoinositides at the Neuromuscular Junction of Drosophila melanogaster: A Genetic Approach
Pages 227-247
Jan R. Slabbaert, Thang Manh Khuong, Patrik Verstreken

Chapter 13 - Devising Powerful Genetics, Biochemical and Structural Tools in the Functional Analysis of Phosphatidylinositol Transfer Proteins (PITPs) Across Diverse Species
Pages 249-302
James M. Davison, Vytas A. Bankaitis, Ratna Ghosh

Chapter 14 - Genome-Wide Screens for Gene Products Regulating Lipid Droplet Dynamics
Pages 303-316
Weihua Fei, Hongyuan Yang

Chapter 15 - The Three Dimensionality of Cell Membranes: Lamellar to Cubic Membrane Transition as Investigated by Electron Microscopy
Pages 317-343
Ketpin Chong, Yuru Deng

Chapter 16 - Quantitative Imaging of Lipid Metabolism in Yeast: From 4D Analysis to High Content Screens of Mutant Libraries
Pages 345-365
Heimo Wolinski, Kristian Bredies, Sepp D. Kohlwein

Chapter 17 - Analysis of Cholesterol Trafficking with Fluorescent Probes
Pages 367-393
Frederick R. Maxfield, Daniel Wüstner

Chapter 18 - Fluorescence Correlation Methods for Imaging Cellular Behavior of Sphingolipid-Interacting Probes
Pages 395-427
Rachel Kraut, Nirmalya Bag, Thorsten Wohland

Chapter 19 - Monitoring Phospholipid Dynamics during Phagocytosis: Application of Genetically-Encoded Fluorescent Probes
Pages 429-444
Helen Sarantis, Sergio Grinstein

Chapter 20 - Genetically Encoded Probes for Phosphatidic Acid
Pages 445-459
Nawal Kassas, Petra Tryoen-Tóth, Matthias Corrotte, Tamou Thahouly, Marie-France Bader, Nancy J. Grant, Nicolas Vitale

Index
Pages 461-476

VOLUMES IN SERIES
Pages 477-486