Genomic technologies provide the means of diagnosis and management of many human diseases. Without insights from cytogenetics, correct interpretation of modern high-throughput results is difficult, if not impossible. This book summarizes applications of cytogenetics and molecular cytogenetics for students, clinicians and researchers in genetics, genomics and diagnostics. The book combines the state-of-the-art knowledge and practical expertise from leading researchers and clinicians and provides a comprehensive overview of current medical and research applications of many of these technologies.
KEY FEATURES
• Provides clear summaries of fluorescence in situ hybridization technologies and others
• Comprehensively covers established and emerging methods
• Chapters from an international team of leading researchers
• Useful for students, researchers and clinicians
Author(s): Thomas Liehr
Series: Medical Genomics and Proteomics
Publisher: CRC Press
Year: 2022
Language: English
Pages: 382
City: Boca Raton
Cover
Half Title
Series
Title
Copyright
Dedication
Contents
Series Preface
Preface
Acknowledgements
Editor
List of Contributors
Chapter 1 Cytogenetics and Chromosomics
Chapter 2 Banding Cytogenetics
Chapter 3 Generation of Microdissection-Derived Painting Probes from Single Copy Chromosomes
Chapter 4 FISH—An Overview
Chapter 5 FISH—Microscopy and Evaluation
Chapter 6 FISH—in Routine Diagnostic Settings
Chapter 7 FISH—in Leukemia Diagnostics
Chapter 8 FISH—in Tissues
Chapter 9 FISH—in Human Sperm and Infertility
Chapter 10 FISH—in Spontaneously Aborted Products of Conception
Chapter 11 FISH—Characterization of Chromosomal Alterations, Recombination, and Outcomes after Segregation
Chapter 12 Multicolor-FISH—Methods and Applications
Chapter 13 FISH—Centromere- and Heterochromatin-Specific Multicolor Probe Sets
Chapter 14 FISH—Detection of Individual Radio Sensitivity
Chapter 15 FISH—Detection of CNVs
Chapter 16 FISH—Interphase Applications Including Detection of Chromosome Instability (CIN)
Chapter 17 FISH—Determination of Telomere Length (Q-FISH/CO-FISH)
Chapter 18 FISH—in Three Dimensions—3D-FISH
Chapter 19 FISH—On Fibers
Chapter 20 FISH—and Single-Cell Gel Electrophoresis Assay (Comet Assay)
Chapter 21 Molecular Karyotyping
Chapter 22 FISH—Mitochondrial DNA
Chapter 23 FISH—in Birds
Chapter 24 FISH—in Fish Chromosomes
Chapter 25 FISH—and the Characterization of Synaptonemal Complex
Chapter 26 RNA-FISH—on Lampbrush Chromosomes: Visualization of Individual Transcription Units
Chapter 27 FISH—in Insect Chromosomes
Chapter 28 FISH—in Plant Chromosomes
Chapter 29 FISH—and CRISPR/CAS9
Index
