Current Topics in Membranes, Volume 63

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This volume of Current Topics in Membranes focuses on Membrane Protein Crystallization, beginning with a review of past successes and general trends, then further discussing challenges of mebranes protein crystallization, cell free production of membrane proteins and novel lipids for membrane protein crystallization. This publication also includes tools to enchance membrane protein crystallization, technique advancements, and crystallization strategies used for photosystem I and its complexes, establishing Membrane Protein Crystallization as a needed, practical reference for researchers.

Author(s): Larry DeLucas (Eds.)
Series: Current Topics in Membranes 63
Edition: 1
Publisher: Academic Press
Year: 2009

Language: English
Pages: 1-305

Content:
Membrane Protein Crystallization
Page ii

Copyright Page
Page iv

Dedication
Page v

Contributors to Volume 63
Pages xi-xiii

Preface
Pages xv-xvi
Larry DeLucas

Contents of Previous Volumes
Pages xvii-xx

Introduction Review Article
Pages 1-3
Larry DeLucas

Chapter 1 Introduction to the Crystallization of Biological Macromolecules Review Article
Pages 5-23
Alex McPherson

Chapter 2 In Vitro Synthesis of Posttranslationally Modified Membrane Proteins Review Article
Pages 25-49
Stefan Kubick, Michael Gerrits, Helmut Merk, Wolfgang Stiege, Volker A. Erdmann

Chapter 3 Harnessing Photosynthetic Bacteria for Membrane Protein Production Review Article
Pages 51-82
Deborah K. Hanson, Donna L. Mielke, Philip D. Laible

Chapter 4 Monoacylglycerols: The Workhorse Lipids for Crystallizing Membrane Proteins in Mesophases Review Article
Pages 83-108
M. Caffrey, J. Lyons, T. Smyth, D.J. Hart

Chapter 5 Practical Aspects of Membrane Proteins Crystallization in Bicelles Review Article
Pages 109-125
Salem Faham, Rachna Ujwal, Jeff Abramson, James U. Bowie

Chapter 6 Membrane Protein Crystallization: Approaching the Problem and Understanding the Solutions Review Article
Pages 127-149
Mads Gabrielsen, Alastair T. Gardiner, Petra Fromme, Richard J. Cogdell

Chapter 7 Tools to Enhance Membrane Protein Crystallization Review Article
Pages 151-178
W. William Wilson, Gregg Whited, Robert W. Payne, Charles Henry, David H. Johnson, Larry DeLucas

Chapter 8 Advances in Microfluidic Membrane Protein Crystallization Techniques Review Article
Pages 179-189
Cory Gerdts, Peter Nollert

Chapter 9 Crystallization of Photosynthetic Membrane Proteins Review Article
Pages 191-227
Petra Fromme, Ingo Grotjohann

Chapter 10 A Practical Guide to X‐Ray Crystallography of β‐barrel Membrane Proteins: Expression, Purification, Detergent Selection, and Crystallization Review Article
Pages 229-267
Mikio Tanabe, Tina M. Iverson

Chapter 11 Bacterial Membrane Proteins: The New Soluble Proteins? Review Article
Pages 269-297
Hubing Lou, Konstantinos Beis, James H. Naismith

Index
Pages 299-305