With over 820 million people facing hunger in today’s world, the need of the hour is the design of plant varieties with high yield, improved traits, and resistance properties in order to mitigate the detrimental impacts of biotic and abiotic stress conditions on food crops. This volume highlights the use of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated nuclease proteins as a solution for sustainable agriculture. CRISPR/Cas-mediated genome editing (GE) provides a robust, simple, precise, efficient, economical, and revolutionary toolbox that leads to improved plant traits with enhanced yield, quality, and resistance against various stresses, including climate-related stress, insect pests and diseases, pollution-related stresses, etc.
Key features of the book
Explains the differences between conventional breeding, genetically modified crops, and genome editing approaches using CRISPR/Cas
Introduces the tools of genome editing, such as ZFNS (zinc finger nucleases), mega nucleases, and TLENS (transcription activator-like effector nucleases)
Covers the history, origin, discovery, structure, and classification of CRISPR/Cas
Examines the databases and computational approaches of CRISPR/Cas
Highlights genome editing of important crop plants with CRISPR/Cas systems, including wheat, maize. tomato, Brassica crops, rice, fruits
Explores potential applications of CRISPR/Cas systems for climate smart crops
Author(s): Deepu Pandita, Anu Pandita
Publisher: CRC Press/Apple Academic Press
Year: 2023
Language: English
Pages: 477
City: Palm Bay
Cover
Half Title
Title Page
Copyright Page
About the Editors
Table of Contents
Contributors
Abbreviations
Preface
Part I: Introduction to Genome Editing Tools
1. Conventional Breeding, Genetically Modified Crops, and Genome Editing
2. An Overview of Tools for Genome Editing: ZFNs, Mega Nucleases, and TALENs
3. Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated (CRISPR/Cas) Systems: Discovery, Structure, Classification, and General Mechanism
4. Computational Tools and Approaches for CRISPR/Cas Technology
Part II: Genome Editing of Crop Plants with CRISPR/Cas Systems: Past, Present, and Future
5. Genome Editing Using CRISPR/Cas9 System for Crop Improvement, Nutrient Enrichment, and Abiotic Stress Tolerance in Rice
6. Genome Editing of Wheat with CRISPR/Cas Systems: Past, Present, and Future
7. Genome Editing of Maize with CRISPR/Cas Systems: Past, Present, and Future
8. Genome Editing of Tomato Crop with CRISPR/Cas Systems: Past, Present, and Future
9. Genome Editing Techniques and Their Applications in Brassica Crops Improvement
Part III: Potential Applications of CRISPR/Cas Systems for Climate Smart Crops
10. CRISPR/Cas-Mediated Genome Editing for Abiotic Stress Tolerance
11. CRISPR/Cas-Mediated Genome Editing for Biotic Stress Tolerance
12. CRISPR/Cas-Mediated Genome Editing for Improvement of Plant Traits
13. Investigation of Plant-Microbe Interactions by CRISPR/Cas for Sustainable Agriculture
14. Unraveling the Application of CRISPR/Cas Systems in Natural Product Research
15. Unraveling the Promise of CRISPR/Cas Systems in Modulating Nutrients and Antinutrients in Fruits
16. CRISPR/Cas-Based Strategies for Resistance Against Plant Viruses
17. Regulatory Status of CRISPR/Cas Genome-Edited Crops
Index
