This book focus on COVID-19 topics, with emphasis on metabolomics and diagnosis. The chapters cover the chemical science for prevention and understanding outbreaks of infectious diseases. This book compiles the most widespread methodologies of application of quality statistical tools added to the evaluation of diagnostic tests for detection of SARS-CoV-2, metabolic behavior of COVID infection severity, and trends in rapid test for COVID-19.
Author(s): Frank N. Crespilho
Publisher: Springer
Year: 2023
Language: English
Pages: 193
City: Cham
Contents
Trends in Electroanalytical Assays for COVID-19 Diagnosis
1 Introduction
1.1 Electrochemical Biosensor Operation
1.2 Structure of SARS-CoV-2
1.3 RT-qPCR Versus Biosensors
2 S Protein-Based Electrochemical Biosensors
3 N Protein-Based Electrochemical Biosensors
4 RNA-Based Electrochemical Biosensors
5 Challenges and Perspectives
References
Microfluidic Devices with Electrochemical Detection Towards Covid-19 Detection
1 A Brief Historic Overview: Types of Substrates, Fabrication Methods and Systems of Detection
2 In a Microfluidic World, Why Does Size Matter?
3 Microfluidic Devices with Electrochemical Detection
4 Electrochemical Biosensing of COVID-19 Using Microfluidics
4.1 COVID-19 Diagnostic Strategies in Bioelectrochemical Microfluidics
4.2 Materials and Immobilization Approaches
5 Detailed Approaches for Electrochemical Detection of COVID-19 Using Microfluidic Devices
References
Carbon-Based Materials for Electrochemical Sensing of SARS-CoV-2
1 Introduction
2 Carbon-Based Materials Applied for SARS-CoV-2 Biosensing
2.1 Macro-Materials: Graphite and Diamond
2.2 Nanostructured Materials
3 Tendencies and Perspectives
References
Electrochemical Immunosensor for Diagnosis of COVID-19
1 Introduction
2 Biomarkers
3 Immunosensors
3.1 Antibodies
3.2 Immobilization Methods
3.3 Immunusensors Format
4 Techniques in Electrochemical Immunosensors
4.1 Voltammetry
4.2 Amperometry
4.3 Electrochemical Impedance Spectroscopy
5 Electrochemical Immunosensors for the Detection of COVID-19
5.1 Electrochemical Detection of SARS-CoV-2 Proteins
5.2 Electrochemical Detection of SARS-CoV-2 RNA
5.3 Electrochemical Detection of SARS-CoV-2 Antibodies
6 Conclusion
References
Optical Fibers Sensors for Detection of SARS-CoV-2 Infection
1 Fundamentals
2 Optical Techniques for Covid-19 Detection
2.1 Surface-Enhanced Raman Spectroscopy (SERS)
2.2 Localized-Surface Plasmon Resonance (LSPR)
2.3 Luminescence
3 Optical Fiber for Biosensing
3.1 Propagation of Light
3.2 Chemically Modified Optical Fiber for Sensing
3.3 Fiber Bragg Grating
3.4 Highlights and Final Comments
4 Perspectives
References
Lateral Flow Assays for COVID-19
1 Introduction
2 LFA Construction
3 LFA for COVID-19
3.1 Nanomaterials for Bioconjugation
3.2 Human Sampling
3.3 Antibody Test
3.4 Antigen Test
3.5 Viral Nucleic Acid Detection
4 General Improvements Using LFA
5 Future Perspectives
References
The Use of NMR Based Metabolomics to Discriminate Patients with Viral Diseases
1 Introduction
1.1 Metabolomics
1.2 NMR as a Tool in Metabolomics
2 Advances in NMR Based Metabolomics to Apply to Diagnostic Diseases
2.1 qNMR Strategies to Overcome the Metabolomics Issues
3 Viral Diseases Discriminated by NMR Based Metabolomics
3.1 Metabolomics Analysis in Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection
3.2 Metabolomics Analysis in Hepatitis C Viral Infection
4 Conclusions and Future Perspectives
References
Application of Quality Statistical Tools for the Evaluation of Diagnostic Tests for SARS-CoV-2 Detection
1 Introduction
2 SARS-CoV-2
2.1 Diagnostic Tests for SARS-CoV-2 Detection
3 Quality Assurance
3.1 Qualitative and Quantitative Methods
3.2 Method Validation
3.3 Uncertainty Estimation
3.4 Proficiency Testing
4 Application of Quality Statistical Tools in Tests for the Detection of SARS-CoV-2
5 Conclusions and Future Perspectives
References
