Elsevier, 2009. - 245 p.
Methods in Cell Biology, Volume 92.
Along with its companion volume on axonemal dynein-mediated motility, this book provides researchers with a comprehensive and up-to-date source of methods for the analysis cilia and flagella, focusing primarily on approaches that have been devised or significantly extended since the last volume of Methods in Cell Biology on this topic (volume 47, 1995). Edited by Stephen M. King and Gregory J. Pazour, the newest installment of this highly acclaimed serial will serve as an essential addition to the study of cilia and flagella.Contents
Contributors
Preface
Bioinformatic Approaches to Dynein Heavy Chain Classification
Identification and Characterization of Dynein Genes in Tetrahymena
Purification of Axonemal Dyneins and Dynein-Associated Components from Chlamydomonas
Purification of Dyneins from Sperm Flagella
Protein Engineering Approaches to Study the Dynein Mechanism using a Dictyostelium Expression System
Biophysical Measurements on Axonemal Dyneins
Protein Electroporation into Chlamydomonas for Mutant Rescue
Analysis of the Role of Nucleotides in Axonemal Dynein Function
The Regulation of Dynein-Driven Microtubule Sliding in Chlamydomonas Flagella by Axonemal Kinases and Phosphatases Candice
Analysis of Redox-Sensitive Dynein Components
Calcium Regulation of Ciliary Motility: Analysis of Axonemal Calcium-Binding Proteins
Isolation and Analysis of Radial Spoke Proteins
Analysis of the Central Pair Microtubule Complex in Chlamydomonas reinhardtii References
Index
Volume in Series
