A widespread appreciation for the role that calcium plays in cell physiology and patho physiology has now been achieved due to the pioneering studies of many of the scientists who attended the VIIth International Spring Symposium on Health Sciences at George Washington University in Washington, D. C. The participants in this unique meeting rep resented diverse fields of basic and clinical research, such as molecular physiology, oncology, molecular genetics, cardiology, bioenergetics, pathology, and endocrinology. The content of the proceedings of the symposium represents work in these and other areas of biomedical research. Organization of the book is aimed at striking a balance between the biochemistry 2 and physiology of normal cell Ca + metabolism and the pathological consequences of al 2 terations in cell Ca + homeostasis. The first section of the book is devoted to the transport mechanisms responsible for 2 2 regulating intracellular Ca + and the pharmacological modalities for controlling cell Ca + . Particular attention is given to the molecular basis for plasma membrane transport activities, 2 2 including the ATP-driven Ca + pump, the Na + -Ca + exchange system, and voltage sensitive 2 Ca + channels. The second section covers the exciting relationships between phosphoinositide metab 2 olism, signal transduction, and cell Ca + metabolism. This section begins with an eloquent overview by Professor Michael Berridge, who was the keynote speaker at the symposium and the recipient of the scientific merit award.
Author(s): Sophie Lotersztajn, Catherine Pavoine, Ariane Mallat, Dominique Stengel, Paul Insel (auth.), Gary Fiskum (eds.)
Series: GWUMC Department of Biochemistry Annual Spring Symposia
Edition: 1
Publisher: Springer US
Year: 1989
Language: English
Pages: 638
Tags: Pharmacology/Toxicology; Biochemistry, general
Front Matter....Pages i-xiii
Front Matter....Pages 1-1
Hormonal Inhibition of the Liver Plasma Membrane (Ca 2+ —Mg 2+ ) ATPase is Mediated by a G s -like Protein....Pages 3-11
The High-Affinity (Ca 2+ —Mg 2+ )ATPase of Rat Liver Plasma Membrane Hydrolyzes Extracellular ATP....Pages 13-19
The Plasma Membrane Calcium Pump....Pages 21-26
The Cardiac Sodium—Calcium Exchange System....Pages 27-31
Molecular Properties of Dihydropyridine Sensitive Calcium Channels....Pages 33-45
Monoclonal Antibodies That Coimmunoprecipitate the 1,4-Dihydropyridine and Phenylalkylamine Receptors and Reveal the Ca 2+ Channel Structure....Pages 47-56
Criteria for Identification of α 1 and α 2 Subunits in a Purified Skeletal Muscle Calcium Channel Preparation....Pages 57-64
Phosphorylation of Dihydropyridine-Sensitive Calcium Channels from Cardiac and Skeletal Muscle....Pages 65-73
Phorbol Esters Stimulate Calcium Influx via Voltage-Dependent Channels in A 7 r 5 Vascular Smooth-Muscle Cells....Pages 75-82
Effects of cAMP-Dependent, Calmodulin-Dependent, and C-Type Protein Kinases on Platelet Calcium Transport....Pages 83-91
Molecular Structure of Canine Cardiac Phospholamban, the Regulatory Protein of Ca Pump ATPase of Sarcoplasmic Reticulum....Pages 93-101
Ca 2+ Transport by Liver and Plant Mitochondria....Pages 103-111
Front Matter....Pages 113-113
Inositol Lipids and Intracellular Communication....Pages 115-124
Receptor-Mediated Ca 2+ Signaling....Pages 125-141
Metabolism of Ins(1,3,4)P 3 by Rat Liver Homogenates....Pages 143-148
Evidence for the Existence of the Inositol Tris/Tetrakisphosphate Pathway in Rat Heart....Pages 149-156
Mechanisms of Intracellular Calcium Movement Activated by Guanine Nucleotides and Inositol-1,4,5-Trisphosphate....Pages 157-167
Activation of the Inositol-1,4,5-Trisphosphate Signaling System by Acute Ethanol Treatment of Rat Hepatocytes....Pages 169-177
Regulation of Hepatic Glycogenolysis by Calcium-Mobilizing Hormones....Pages 179-185
Receptor Desensitization Is a Major Regulator of Hormone-Induced Ca 2+ Mobilization in the Human Platelet....Pages 187-192
Front Matter....Pages 113-113
Specific Receptors for Inositol 1,4,5-Trisphosphate in Endocrine Target Tissues....Pages 193-203
Intracellular Calcium Transients Associated with Endothelium-Derived Relaxing Factor Release May Be Mediated by Inositol-1,4,5-Trisphosphate....Pages 205-210
Protein Kinase C in Rat Skeletal Muscle....Pages 211-219
Activation of Phosphoinositide-Specific Phospholipase C by Ligands in the Presence of Guanine Nucleotides....Pages 221-230
Guanine Nucleotide-Dependent Release of Arachidonic Acid in Permeabilized Inflammatory Cells....Pages 231-236
Front Matter....Pages 237-237
Measurement of Free Intracellular Calcium (Ca i ) in Fibroblasts....Pages 239-248
Growth Factors, Oncogenes, and Protein Kinase C....Pages 249-254
Insulin-like Growth Factor II Stimulates Calcium Influx in Competent Balb/c 3T3 Cells Primed with Epidermal Growth Factor....Pages 255-264
Small Cell Lung Cancer Bombesin Receptors Utilize Calcium as a Second Messenger....Pages 265-272
Extracellular Ca 2+ and Cell Cycle Transitions....Pages 273-282
Role and Properties of Ligand-Induced Calcium Fluxes in Lymphocytes....Pages 283-291
Modulation of c- fos and c- myc Expression by Effectors of Ion Movements....Pages 293-303
Regulation of c- fos Expression by Voltage-Dependent Calcium Channels....Pages 305-312
Calcium Pulses, Waves, and Gradients in Early Development....Pages 313-321
Front Matter....Pages 323-323
Calcium Ions, Hormones, and Mammalian Oxidative Metabolism....Pages 325-330
Regulation of Pyruvate Dehydrogenase in Isolated Cardiac Myocytes and Hepatocytes by Cytosolic Calcium....Pages 331-341
Calcium as a Hormonal Messenger for Control of Mitochondrial Functions....Pages 343-349
Effect of Mitochondrial Ca 2+ on Hepatic Aspartate Formation and Gluconeogenic Flux....Pages 351-360
Regulatory Effects of a Thromboxane A 2 Analogue on Hepatic Glycogenolysis and Vasoconstriction....Pages 361-368
Metabolic Regulation of Ca 2+ Handling in Permeabilized Insulinoma Cells....Pages 369-377
Front Matter....Pages 323-323
Calcium, Calmodulin, and Insulin Action in the Adipocyte....Pages 379-385
Role of Calcium in the Regulation of Mammalian Lipoxygenases....Pages 387-396
Phosphorylation and Modulation of Enzymic Activity of 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase by Multiple Kinases....Pages 397-409
Origin and Role of Calcium in Platelet Activation-Contraction-Secretion Coupling....Pages 411-425
Calmodulin-like Ca 2+ -Binding Proteins of Smooth Muscle....Pages 427-437
Front Matter....Pages 439-439
Role of Ion Regulation in Cell Injury, Cell Death, and Carcinogenesis....Pages 441-449
Role of Calcium in Oxidative Cell Injury....Pages 451-461
Cytosolic Free Calcium and Cell Injury in Hepatocytes....Pages 463-470
Mechanisms of Anoxic Injury to Transport and Metabolism of Proximal Renal Tubules....Pages 471-477
X-Ray Microprobe Analysis of Subcellular Elemental Distribution in Normal and Injured Peripheral Nerve Axons....Pages 479-489
Prevention of Ischemic Brain Mitochondrial Injury by Lidoflazine....Pages 491-498
Role of Phosphoinositides in the Response of Mammalian Cells to Heat Shock....Pages 499-505
Cell Calcium in Malignant Hyperthermia....Pages 507-511
Pathogenetic Roles of Intracellular Calcium and Magnesium in Membrane-Mediated Progressive Muscle Degeneration in Duchenne Muscular Dystrophy....Pages 513-525
Front Matter....Pages 527-527
Characteristics and Functional Implications of Spontaneous Sarcoplasmic Reticulum-Generated Cytosolic Calcium Oscillations in Myocardial Tissue....Pages 529-543
Phospholipid-Calcium Relations at the Sarcolemma of the Cardiac Cell....Pages 545-550
Role of Membrane Dysfunction and Altered Calcium Homeostasis in the Pathogenesis of Irreversible Myocardial Injury....Pages 551-559
Ischemic Alterations in Mitochondrial Calcium Transport Kinetics....Pages 561-570
Structural, Biochemical, and Elemental Correlates of Injury in Cultured Cardiac Cells....Pages 571-580
Interaction of Volatile Anesthetics with Calcium-Sensitive Sites in the Myocardium....Pages 581-591
Front Matter....Pages 527-527
Cardiac Protection by Halothane Following Ischemia and Calcium Paradox....Pages 593-601
Defective Ca 2+ Functions in Protein (47-kDa) Phosphorylation and in the Coupling to Physiological Responses in Platelets from Stroke-Prone Spontaneously Hypertensive Rats....Pages 603-615
Effects of Elastin Peptides on Ion Fluxes....Pages 617-622
Back Matter....Pages 623-642
