Content: A survey of metabolic transformations of [delta]¹-tetrahydrocannabinol / Sumner Burstein --
GLC and HPLC analyses of cannabinoids in biological fluids and applications / E.R. Garrett, A.J. Gouyette, and C.A. Hunt --
Detection and quantitation of tetrahydrocannabinol in physiological fluids / Monroe E. Wall, Dolores R. Brine, Joan T. Bursey, and David Rosenthal --
Quantitation of [delta]⁹-tetrahydrocannabinol and 11-nor-[delta]⁹-tetrahydrocannabinol-9-carboxylic acid in body fluids by GC/CI-MS / R.L. Foltz, P.A. Clarke, B.J. Hidy, D.C.K. Lin, A.P. Graffeo, and B.A. Petersen --
Improvement of the mass fragmentographic technique for quantification of tetrahydrocannabinol in human blood plasma / Agneta Ohlsson, Stig Agurell, Jan-Erik Lindgren, and Kurt Leander --
A general approach to the analysis of cannabinoids from physiological sources / J. Rosenfeld --
Quantitation of [delta]⁹-tetrahydrocannabinol and its metabolites in human urine by probability based matching GC/MS / Donald E. Green, Fu-Chuan Chao, Kay O. Loeffler, and Saul L. Kanter --
HPLC analysis of [delta]⁹-tetrahydrocannabinol and metabolites in biological fluids / Seth R. Abbott, John R. Berg, Kay O. Loeffler, Saul Kanter, Leo E. Hollister, Joan Hawkins Abrams, Hugh L. Baras, and Reese T. Jones --
Radioimmunoassay of cannabinoid compounds / C.E. Cook --
Antisera raised against tetrahydrocannabinol in the radioimmunoassay of cannabinoids / J.D. Teale, Jacqueline M. Clough, L.J. King, V. Marks, P.L. Williams, and A.C. Moffat --
HPLC analyses of [delta]⁹-tetrahydrocannabinol and 11-nor-[delta]⁹-tetrahydrocannabinol-9-carboxylic acid in human plasma / Jimmie L. Valentine, Owen H.M. Gan, H.C. Nio, and Everett D. Thompson --
Detection and quantitation of [delta]⁹-tetrahydrocannabinol in plasma by dansylation and double labeling / J.M. Scherrmann, R. Bourdon, H. Hoellinger, Nguyen-Hoang Nam, and E. Fournier.
Author(s): Joe A. Vinson (Eds.)
Series: ACS Symposium Series 98
Publisher: American Chemical Society
Year: 1979
Language: English
Pages: 238
City: Washington
Title Page
......Page 1
Half Title Page......Page 3
Copyright......Page 4
ACS Symposium Series......Page 5
FOREWORD......Page 6
PdftkEmptyString......Page 0
PREFACE......Page 7
1 A Survey of Metabolic Transformations of Δ1-Tetrahydrocannabinol......Page 8
REFERENCES......Page 18
2 GLC and HPLC Analyses of Cannabinoids in Biological Fluids and Applications......Page 20
Spectrophotometric Determination of the pK'a(7)......Page 22
Glassbinding......Page 24
Purification of Cannabinoids by High Pressure Liquid Chromatography (HPLC) (15,16)......Page 26
Effect of HPLC Separation on GLC Analysis of Δ9-Tetrahydrocannabinol in Plasma (15)......Page 30
Stability of Tetrahydrocannabinol in Aqueous Solutions (16,18)......Page 33
Equivalency of Radiochemical Analyses of 14C-Δ9-Tetrahydrocannabinol and GLC Electron Capture Detection of Derivatized Material after Normal Phase HPLC in Dog Plasma (14)......Page 38
Pharmacokinetic Studies in Dogs......Page 40
CONCLUSIONS......Page 41
REFERENCES......Page 43
3 Detection and Quantitation of Tetrahydrocannabinolin Physiological Fluids......Page 45
Internal Standard......Page 47
Analysis of Δ9-THC, Cannabinol, and 11-OH-Δ9-THC......Page 48
RESULTS FOR Δ9-THC, CANNABINOL, AND 11-OH-Δ9-THC......Page 50
Extraction and Purification......Page 53
GLOMS Analysis......Page 56
RESULTS FOR 11-NOR-Δ9-THC-9-CARBOXYLIC ACID......Page 57
Internal Standards......Page 59
Metabolic and Pharmacokinetic Data......Page 60
Comparison of GLC-MS With Other Procedures......Page 61
REFERENCES......Page 62
CURRENT METHODOLOGY FOR QUANTITATION OF Δ9-THC......Page 64
High Fressure Liquid Chromatography Clean-up.......Page 70
Separation by Glass Capillary Gas Chromatography......Page 72
Quantitation Using CI Mass Spectrometry and Selected Ion Monitoring.......Page 73
REFERENCES......Page 76
METHODS......Page 77
ANALYSIS OF THC IN BLOOD PLASMA......Page 78
MASS FRAGMENTOGRAPHY......Page 79
RESULTS AND DISCUSSION......Page 80
REFERENCES......Page 82
6 A General Approach to the Analysis of Cannabinoids from Physiological Sources......Page 84
REFERENCES......Page 94
7 Quantitation of Δ9-Tetrahydrocannabinol and Its Metabolites in Human Urine by Probability Based Matching GC/MS......Page 95
Instrumentation......Page 104
Procedure......Page 105
RESULTS AND DISCUSSION......Page 109
CONCLUSIONS......Page 113
REFERENCES......Page 114
8 HPLC Analysis of Δ9-Tetrahydrocannabinol and Metabolites in Biological Fluids......Page 116
HPLC......Page 118
Results......Page 119
1. Hexane Extract, Subject 1......Page 120
2. Hexane Extract, Subject 2......Page 123
3. EII Extract, Subject 1......Page 126
E-I Ether Extract, Subject 1......Page 127
B. Discussion......Page 129
Results......Page 130
Discussion......Page 134
REFERENCES......Page 136
9 Radioimmunoassay of Cannabinoid Compounds......Page 138
IMMUNOGEN SYNTHESIS - GENERAL PRINCIPLES......Page 140
IMMUNOGEN SYNTHESIS -Δ8-and Δ9-THC......Page 141
ANTISERUM CHARACTERIZATION......Page 144
Δ9-THC ANTISERUM......Page 147
IODINATED RADIOLIGAND......Page 149
REFERENCES......Page 154
10 Antisera Raised Against Tetrahydrocannabinol in the Radioimmunoassay of Cannabinoids......Page 156
Antibody Production......Page 157
Applications of RIA......Page 163
High Pressure Liquid Chromatography (HPLC) and RIA......Page 168
REFERENCES......Page 173
11 HPLC Analyses of Δ9-Tetrahydrocannabinol and 11-Nor-Δ9-tetrahydrocannabinol-9-carboxylic Acid in Human Plasma......Page 175
NORMAL PHASE HPLC......Page 177
REVERSE PHASE HPLC......Page 193
SIMULTANEOUS ASSAY OF I AND VI......Page 202
CONCLUSIONS......Page 203
REFERENCES......Page 204
12 Detection and Quantitation of Δ9-Tetrahydrocannabinol in Plasma by Dansylation and Double Labeling......Page 206
PRINCIPLES OF THE METHOD......Page 207
B. Esterification, purification and counting......Page 208
1. Temperature and reaction time.......Page 209
3. 14C DANS-C1 excess (Fig. 4)......Page 211
C. Hydrolysis and Extraction......Page 214
A. Sensitivity......Page 215
B. Specificity......Page 216
CONCLUSIONS......Page 220
REFERENCES......Page 221
Β......Page 223
C......Page 224
D......Page 226
G......Page 227
H......Page 228
L......Page 229
M......Page 230
Ρ......Page 231
S......Page 233
Τ......Page 234
U......Page 237
W......Page 238
