Charter 10 of book: InTech, 2011. — 428 p. — ISBN 978-953-307-730-7.In recent years it has become clear that breast cancer is not a single disease but rather that the term encompasses a number of molecularly distinct tumors arising from the epithelial cells of the breast. There is an urgent need to better understand these distinct subtypes and develop tailored diagnostic approaches and treatments appropriate to each. This book considers breast cancer from many novel and exciting perspectives. New insights into the basic biology of breast cancer are discussed together with high throughput approaches to molecular profiling. Innovative strategies for diagnosis and imaging are presented as well as emerging perspectives on breast cancer treatment. Each of the topics in this volume is addressed by respected experts in their fields and it is hoped that readers will be stimulated and challenged by the contents.Contents of charter:
1. Introduction.
1.1 Cell signalling and tumorigenesis.
1.2 Correlation between tumor secreted proteins and cancer.
1.3 Detection of breast cancer markers with high-throughput technologies.
1.4 Utilization of 2D-DIGE / MALDI-TOF MS-based strategies in the global analysis of breast cancer markers.
1.5 Application of luminal epithelial cell models with various invasive stages in the discovery of breast cancer markers.
2. 2D-DIGE and MALDI-TOF MS analysis of secretomes across MCF-10A, MCF-7 and MDA-MB-231 cells.
3. 2D-DIGE and MALDI-TOF MS analysis of total intracellular proteomes across MCF-10A, MCF-7 and MDA-MB-231 cells.
4. Validation of characterized breast cancer markers through immunoblotting and immunofluorescence.
5. Validation of unreported identified putative tumorigenic markers through immunoblotting and immunofluorescence.
6. Functional classifications of the identified breast cancer markers.
7. Different proteomic approaches in the study of breast cancer markers.
8. Conclusion.
9. Acknowledgments.
10. References.
