Bone Marrow Diagnosis, Third Edition, is an essential resource for pathologists and haematologists who need to report bone marrow trephine biopsies.
Practical and highly illustrated this edition has been comprehensively updated whilst remaining succinct and concentrating on the core information necessary to make an accurate diagnosis.
The text provides comparisons of the common methods of sample collection, fixation and staining, and a clear description of how to examine a trephine section. Applying a consistent approach, the chapters cover the range of disorders of bone marrow, discussing the clinical features, histopathology of bone marrow and diagnostic problems of each condition. Each chapter closes with a summary of key points and each diagnostic entity is accompanied by high quality images, over 900 in all, showing typical and more unusual examples of histological features.
This compact text, oriented at diagnosis and comprehensively accompanied by usable illustrations, is an invaluable reference tool for the trainee and practicing histopathologists, pathologists and haematologists.
A practical guide aimed at allowing a busy pathologist to easily find the essential description and illustration of the most common bone marrow diseases seen in trephines
Covers new treatment for chronic myeloid leukaemia, B-cell lymphoma and antibody treatments
High quality colour images accompany each diagnostic entity
Coverage of cytology in sections relating to myeloid dysplasias and acute leukaemias
Addresses lymphoma categorization and individual lymphoma entities
Incorporates new WHO classifications of lymphomas and leukaemias
Author(s): Kevin Gatter, David Brown
Edition: 3
Publisher: Wiley-Blackwell
Year: 2014
Language: English
Pages: 227
Tags: Pathology, Medicine
Bone Marrow Diagnosis: An Illustrated Guide
Cover
Title page
Copyright page
Contents
Preface to the third edition
Preface to the first edition
CHAPTER 1: Introduction
Reasons for performing bone marrow biopsies
How to examine a trephine section
References
CHAPTER 2: The normal bone marrow
Site of haematopoiesis
Components of the normal bone marrow trephine
Bone
Stroma
Haematopoietic tissue
Other cells
References
CHAPTER 3: Infections including human immunodeficiency virus
Bacterial infection
Viral infection
HIV infection
Clinical features
Histopathology of the bone marrow
Reactive features
Secondary infections
Malignancies
Diagnostic problems
Reference
CHAPTER 4: Anaemias and aplasias
Anaemias
Iron deficiency anaemia
Copper deficiency anaemia
Anaemia of chronic disease
Haemolytic anaemia
Megaloblastic anaemia
Congenital dyserythropoietic anaemias
Aplasias
Acquired aplastic anaemias
Congenital aplastic anaemias
References
CHAPTER 5: The myelodysplastic syndromes
Myelodysplasia
Clinical features
Histopathology of the bone marrow
Cellularity
Abnormalities of marrow architecture
Dysmegakaryopoiesis
Dyserythropoiesis
Dysgranulopoiesis
Abnormally located immature precursors
Histological correlation with the WHO and FAB classifications
Chronic myelomonocytic leukaemia
Clinical features
Diagnosis
Histopathology of the bone marrow
Diagnostic problems
Differential diagnosis
Secondary MDS
Overlap between myelodysplasia and myeloproliferation
References
CHAPTER 6: Myeloproliferative neoplasms
Classification of MPN
Chronic myeloid leukaemia
Clinical features
Histopathology of the bone marrow
Diagnostic problems
Polycythaemia vera
Clinical features
Histopathology of the bone marrow
Diagnostic problems
Essential thrombocythaemia
Clinical features
Histopathology of the bone marrow
Diagnostic problems
Primary myelofibrosis
Clinical features
Histopathology of the bone marrow
Acute myelofibrosis
Diagnostic difficulties
Mast cell disease
Histopathology of the bone marrow
Differential diagnosis
Transient myeloproliferative disorder
Myeloproliferative neoplasms with eosinophilia and rearrangements of PDGFRA, PDGFRB or FGFR1
References
CHAPTER 7: Acute leukaemia
Classification
Acute myeloid leukaemia
Classification
Histopathology and immunophenotyping
Diagnostic problems
Acute lymphoblastic leukaemia
Transplantation and post-transplantation lymphoproliferative disorders
References
CHAPTER 8: Lymphomas: an overview
What does the clinician require from the pathologist?
B cell neoplasms in the WHO classification
Precursor B-cell neoplasm
Mature B-cell neoplasms
T cell and natural killer cell neoplasms in the WHO classification
Hodgkin lymphoma
References
CHAPTER 9: Precursor B and T lymphoblastic leukaemia (acute lymphoblastic leukaemia) and lymphoblastic lymphoma
Histopathology of the bone marrow
Cytogenetics of B lymphoblastic leukaemia/lymphoma
Cytogenetics of T lymphoblastic leukaemia/lymphoma
Diagnostic problems
Sparse infiltrate
Distinction from chronic lymphocytic leukaemia
Distinction from AML
Detection of early disease and relapse
References
CHAPTER 10: Mature B cell neoplasms
Chronic lymphocytic leukaemia/small lymphocytic lymphoma incorporating B cell and T cell prolymphocytic leukaemia
Clinical features
Histopathology of the bone marrow
Genetics
Diagnostic problems
References
Lymphoplasmacytic lymphoma including Waldenström’s macroglobulinaemia
Histopathology of the bone marrow
References
Mantle cell lymphoma
Histopathology of the bone marrow
Cytogenetics
Diagnostic problems
References
Follicular lymphoma
Histopathology of the bone marrow
Diagnostic problems
Cytogenetics and molecular biological features
The effect of rituximab treatment
References
Marginal zone B-cell lymphoma (including MALT type)
Clinical features
Histopathology of the bone marrow
Diagnostic problems
References
Hairy cell leukaemia
Clinical features
Histopathology of the bone marrow
Patterns of involvement
Diagnostic problems
Hairy cell leukaemia variant
References
Multiple myeloma
Clinical features
Histopathology of the bone marrow
Immunophenotype
Cytogenetics
Less common appearances
Other features occasionally seen in association with myeloma
Diagnostic problems
References
Diffuse large B cell lymphoma
Histopathology of the bone marrow
Cytogenetics
Diagnostic problems
References
Burkitt lymphoma
Histopathology of the bone marrow
Cytogenetics
Diagnostic problems
Necrotic tumours
CHAPTER 11: Mature T and NK cell neoplasms
T-cell large granular lymphocytic leukaemia
Histopathology of the bone marrow
Immunophenotype
Aggressive NK cell leukaemia
Clinical features
Histology
Immunophenotype
Cytogenetics
Prognosis and treatment
References
Cutaneous T cell lymphoma
General features
Histopathology of the bone marrow
Diagnostic problems
Reference
Peripheral T cell lymphomas, not otherwise specified
Histopathology of the bone marrow
Diagnostic problems
Hepatosplenic T cell lymphoma
Histopathology of the bone marrow
Diagnostic problems
Angio-immunoblastic T cell lymphoma
Histopathology of the bone marrow
Diagnostic problems
Adult T cell lymphoma/leukaemia
Histopathology of the bone marrow
Diagnostic problems
Anaplastic large cell lymphoma
ALCL ALK-positive
Histopathology of the bone marrow
Immunophenotype
Diagnostic problems
Cytogenetics
ALCL ALK-negative
References
CHAPTER 12: Hodgkin lymphoma
Clinical features
Histopathology of the bone marrow
Diagnostic problems
CHAPTER 13: Metastatic disease
Clinical features
Histopathology of the bone marrow
Carcinoma
Paediatric tumours
Diagnostic problems
Other fibrosing conditions
Reference
CHAPTER 14: Bone, stroma and miscellaneous changes
Bone
Osteoporosis
Hyperparathyroidism
Renal osteodystrophy
Paget’s disease
Osteopetrosis
Stroma
Fibrosis
Lymphoid nodules
Granulomas
Serous atrophy
Vasculitis
Foamy macrophages
Miscellaneous changes
Amyloidosis
Immune thrombocytopenic purpura
Thrombotic thrombocytopenic purpura
Erythrophagocytosis
Haemosiderin
Necrosis
The effects of drugs and chemicals
References
CHAPTER 15: Technical considerations
Preparation of bone marrow biopsies
Fixation and decalcification
Aspirates
Trephines
Processing and embedding
Staining of bone marrow biopsies
Giemsa stain
Immunohistochemistry
The value of immunostaining methods
Antibodies for diagnostic use and the CD system
index
Index
