Corrosion causes great losses to the economy every year. Microbial-influenced corrosion (MIC) is important for maritime, chemical engineering, and bioprocess engineering industries. Presently no environmentally friendly technology has been available to minimize the economic loss of biocorrosion. Currently available anti-biocorrosion technology depends heavily on chemical methods to regulate biofilm formation which has a negative impact on the environment. Therefore, it is essential to know the fundamentals of the roles of biomolecules within the whole process to develop detailed research capabilities and potential control and management strategies. This book targets the roles of EPS, proteins, lipids, DNA, and different metabolites currently known to be involved in the corrosion processes. The potential roles of EPS, proteins, lipids and enzymes are still poorly understood. There are still collective issues that need to be addressed, including the importance of the microbial role in MIC. More specifically, there exists a need to understand the impact of enzyme activities inside the biofilm matrix on the dynamics of corrosion reactions. Also, there is the involvement of metals or organometallic complexes in electron transfer and from chemically and morphologically diverse metallic surface films to ultimate electron acceptors.
Author(s): Santosh Kumar Karn, Anne Bhambri
Series: Biochemistry Research Trends
Publisher: Nova Science Publishers
Year: 2023
Language: English
Pages: 151
City: New York
Contents
Preface
Acknowledgments
Chapter 1
Corrosion: An Overview
Abstract
Introduction
Microbiologically Influenced Corrosion (MIC)
Mechanisms
Cathodic Depolarization by Hydrogenase
Iron Sulphides (King’s Mechanism)
Volatile Compound of Phosphorous
Anodic Depolarization
Fe-Binding Exopolymers
Sulphide and Hydrogen-Induced Stress Corrosion Cracking (SCC)
Role of Sulphides
Three Stages Mechanism (Romero Mechanism)
Biocatalytic Cathodic Sulphate Reduction (BCSR)
Economical Loss from Corrosion
Chapter 2
The Role of Biofilm in Corrosion
Abstract
Introduction
Biofilm in Industries and Their Significance
Places Where Biofilm Forms
Organisms Involved in Biofilm Formation
Mechanisms of Biofilm Formation
Economical Loss Due to Biofilm Formation
Chapter 3
Microbial Diversity and Their Importance
Abstract
Introduction
Microbial Diversity
Microorganisms Involved in the Corrosion Process
Iron-Oxidizing Bacteria
Iron-Reducing Bacteria
Manganese-Oxidizing Bacteria
Manganese-Reducing Bacteria
Sulphur-Reducing or Oxidizing Bacteria
Chapter 4
Techniques to Determine the Microbial Diversity
Abstract
Introduction
Morphological Characterization of Bacteria
Biochemical Characterization
Fatty Acid Methyl Ester (FAME) Analysis
Molecular Methods to Determine Microbial Diversity
Molecular-Based Approach for Analyzing Microbial Diversity
Amplified Ribosomal Restriction DNA Analysis (ARDRA)
Random Amplification of Polymorphic DNA (RAPD)
REP-PCR (BOX Element)
Ribosomal Intergenic Spacer Analysis (RISA)/ Automated Intergenic Spacer Analysis (ARISA)
Single-Strand Conformation Polymorphisms (SSCP)
PCR-Independent Approaches
DNA-Reassociation Kinetics and DNA: DNA Hybridization
DNA Microarrays
Reverse Sample Genome Probing (RSGP)
Phylogenetic Analysis and 16S rDNA Gene Sequencing
Next‐Generation Sequencing
Using the Flow Cytometry Method to Determine Microbial Diversity
Using the Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI)-Based Method to Determine Microbial Diversity
Chapter 5
Extracellular Polymeric Substances and Corrosion
Abstract
Introduction
EPS: An Overview
Source of EPS
EPS in the Corrosion Process
Techniques to Determine EPS
Chapter 6
Proteins in Corrosion
Abstract
Introduction
Proteins: An Overview
Enzymes: An Overview
Role of Proteins and Enzymes in the Corrosion Process
Techniques to Determine Enzymes in Corrosion
Enzyme-Influenced Oxygen Reduction
Pivotal Role of Hydrogen Peroxide
Enzymes Producing H2O2: Oxidases
Microbial-Induced Deposition of Mn Oxides
Chapter 7
Role of Lipids in Corrosion
Abstract
Introduction
Lipids: An Overview
Role of Lipids in Corrosion Process
Sample Preparation
Separation and Analysis by Chromatography
Lipid Fractions by TLC
Fatty Acid Methyl Esters by GC
Chemical Techniques
Chapter 8
DNA or eDNA (Environmental DNA) in Corrosion Process
Abstract
Introduction
DNA or eDNA
eDNA: A Source For Detection of New Species
eDNA in the Corrosion Process
Chapter 9
Technique to Identify Biomolecules or Biofilm in Corrosion
Abstract
Introduction
Confocal Laser Scanning Microscopy (CLSM)
Determination of Dry Weight and Total Carbohydrate Levels
Analysis of Metal Ion Leaching and Accumulation by Biofilm
Microscopic Analysis of Biofilm
SEM and Energy-Dispersive X-Ray Analysis
Scanning Electron Microscopy (SEM) Analysis
Weight Loss of Carbon Steel (Corrosion Rates)
Identification of Biofilm Bacteria
Chapter 10
Omics Approach in Biocorrosion
Abstract
Omics Approach
Metabolomics: An Approach
Metabolic Fingerprinting
Metabolomics in Biocorrosion
Analysis of Metabolic Foot Printing
References
Index
About the Authors
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