Biomimetic Protein Based Elastomers: Emerging Materials for the Future

Cover
Dedication
Preface
Contents
Chapter 1 Biomimetic Protein-based Elastomers: Emerging Materials for the Future
1.1 Introduction
Chapter 2 Native Resilin: Properties, Occurrence and Biological Functions of a Remarkable Bio- elastomer
2.1 Native Resilin – The Pliant Protein
2.2 Occurrence and Functions of Resilin in Different Arthropod Exoskeleton Systems
2.2.1 Arthrodial Membranes
2.2.2 Legged Locomotion
2.2.3 Flight Systems: Folds, Tendons and Microjoints
2.2.4 Attachment Systems
2.2.5 Mouthparts
2.2.6 Reproductive Organs, Mechanoreceptors and Compound Eyes
2.3 Conclusion
Acknowledgements
References
Chapter 3 Design and Production of Chimeric Resilin-like Polypeptides (RLPs)
3.1 Introduction
3.2 Why Chimeric RLPs?
3.3 Different Design Schemes of Chimeric RLPs
3.3.1 Chimeric RLPs Containing Bioactive Peptide Sequences
3.3.2 Chimeric RLPs Comprising Peptide Sequences for Tuning Mechanical Properties
3.3.3 Chimeric RLPs Comprising Structural Protein Sequences
3.4 Production of Chimeric RLPs
3.5 Mechanical Properties of Chimeric RLPs
3.6 Potential Applications of Chimeric RLPs
3.7 Future Perspectives
References
Chapter 4 Intrinsically Disordered and Resilin-based Protein Polymers for High-performance Biomaterials Applications
4.1 Intrinsically Disordered Proteins (IDPs)
4.2 Structural Proteins: Naturally Occurring IDPs
4.3 Recombinantly Derived Intrinsically Disordered Protein Polymers (IDPPs)
4.3.1 Mechanical Properties and Advantages
4.3.2 Thermally Responsive Properties and Advantages
4.4 IDPPs in High- performance Materials
4.5 IDPPs in Structured Biomaterials
4.5.1 IDPPs as Building Blocks for Self-assembled Structures
4.5.2 IDPPs in Microstructured Hydrogels
4.6 IDPPs for Tissue Engineering Applications
4.6.1 Vocal Fold Tissue Engineering
4.6.2 Cardiovascular Tissue Engineering
4.6.3 Cartilage Tissue Engineering
4.7 Conclusions and Perspectives
References
Chapter 5 Resilin-mimetic Polypeptides and Elastomeric ModularProtein Polymers: Amino Acid Sequence, Conformational Ensemble, and Stimuli Responsiveness
5.1 Introduction
5.2 Synthesis and Purification
5.3 Amino Acid Sequence, Composition, and Molecular Structure Prediction
5.4 Structural Investigation by Experimental Methods
5.4.1 Circular Dichroism (CD) Spectroscopy
5.4.2 Fourier-transform Infrared Spectroscopy
5.4.3 NMR Spectroscopy
5.4.4 Small-angle Scattering
5.5 Multistimuli Responsiveness
5.5.1 Temperature and Ion Responsiveness
5.5.2 pH and Light Responsiveness
5.5.3 Responsiveness to Different Substrate Surfaces
5.5.4 Mechanical Responsiveness
5.6 Modular Self-assembly
5.7 Conclusions
5.8 Outlook
Abbreviations
Acknowledgements
References
Chapter 6 Advanced Biomedical Applications of Self-assembled Elastin-like Recombinamer Structures
6.1 Introduction
6.2 Elastin-like Recombinamers (ELRs): Definition, Description, and Characteristics
6.3 Self-assembly in ELRs
6.3.1 Concept of Self-assembly
6.3.2 Morphologies of Self-assembled ELR Structures
6.4 ELRs as Intrinsically Disordered Proteins andTheir Impact on Self-assembled Structures: Concept and Characteristics
6.4.1 Concept of Intrinsic Protein Disorder
6.4.2 ELRs as Intrinsically Disordered Proteins
6.4.3 Example
6.5 Biomedical Applications of Self-assembled ELR Structures
6.5.1 Drug Delivery
6.5.2 Tissue Engineering
6.5.3 Biomedical Devices
6.6 Conclusions
References
Chapter 7 Smart Nanofibrous Materials for Tissue Engineering
7.1 Introduction
7.2 Self-assembling Peptides and Protein Nanofibers
7.2.1 a-Helical Coiled- coil Nanofibers
7.2.2 Elastin and Elastin-like Polypeptide Nanofibers
7.2.3 Silk and Silk- like Protein Nanofibers
7.2.4 Collagen and Collagen-mimetic Peptides
7.3 Polymer- based Smart Nanofibers
7.3.1 pH-responsive Nanofibers
7.3.2 Thermoresponsive Nanofibers
7.3.3 Electroresponsive Nanofibers
7.3.4 Piezoelectric Nanofibers
7.3.5 Magnetoresponsive Nanofibers
7.4 Conclusion
Acknowledgements
References
Chapter 8 Biomimetic Adhesives for Clinical Applications
8.1 Clinical Applications of Surgical Adhesives
8.1.1 Clinical Need for Surgical Adhesives
8.1.2 Key Design Requirements – Performance and Safety
8.1.3 FDA Regulation of Surgical Adhesives
8.1.4 FDA Guidance for Testing of Surgical Adhesives
8.2 Natural Biomimetic Adhesives
8.2.1 Biomimetic Adhesion
8.2.2 Pressure-sensitive Adhesion
8.2.3 Mechanical Adhesion
8.2.4 Dry Adhesion
8.2.5 Intermolecular Interactions
8.3 Mussel-inspired Adhesion
8.3.1 Mussel Adhesive Chemistry
8.3.2 Mussel Foot Proteins (mfps)
8.3.3 Commercial Production of mfps
8.4 Nature-inspired Surgical Adhesives for Soft Tissues
8.4.1 Materials for Surgical Adhesives
8.4.2 Synthetic Polymer- based Adhesive Biomaterials
8.4.3 Protein-based Adhesive Biomaterials
8.4.4 Conclusions and Future Directions
Acknowledgements
References
Chapter 9 Silk-elastin-like Protein-based Polymers for Controlled Delivery Applications
9.1 Introduction
9.1.1 Structure–Function Relationships
9.1.2 Physical Characterization ofHydrogel-forming Silk-elastin-like Protein Polymers (SELPs)
9.1.3 Recombinant Synthesis, Expression, and Purification
9.2 Controlled Release From (SELPs)
9.2.1 Adenoviral Delivery
9.2.2 Controlled Release of Synthetic Glycosaminoglycans
9.3 SELPs as Embolics, Chemoembolics, and Embolization Visualization Systems
9.3.1 Chemoembolic SELPs
9.3.2 SELP Embolics for Fluorescence Image-guided Surgery
9.4 Ophthalmic Application
9.5 Micellar-like SELP Particles
9.5.1 Mucoadhesive SELP Nanoparticles
9.6 Three-dimensional Nanoparticle Networks
9.7 Cellular Delivery
9.8 Summary and Future Directions
Abbreviations
Acknowledgements
References
Chapter 10 Processing and Properties of Regenerated Bombyx mori Silk and Recombinant Spider Silk Fibers
10.1 Introduction
10.2 Production/Preparation and Preconditioning of Silk Materials for Technical Processing
10.2.1 Regeneration and Preconditioning of B. mori Fibroin
10.2.2 Recombinant Production of Spider Silk Proteins
10.2.3 Preparation and Conditioning of Silk Solutions for Technical Processing
10.3 Artificial Silk Fibers and Nonwovens
10.3.1 Wet Spinning
10.3.2 Dry Spinning
10.3.3 Microfluidic Spinning
10.3.4 Electrospinning
10.4 Conclusion and Future Prospects
References
Chapter 11 Protein, Biomimetic Protein, and DesignerPeptide-directed Synthesis of Metal Nanoparticles,Metal Nanoclusters and Nanobioconjugates, and Their Potential Applications
11.1 Introduction
11.2 Nanoparticles (NPs) and Nanoclusters (NCs): Effect of the Size and the Shape
11.3 Statistics and Network Visualization
11.4 Nanoparticles and Nanoclusters— A Historical Perspective and the Fundamental Principles
11.4.1 Metal Nanoparticles (m-NPs) and Localized Surface Plasmon Resonance
11.4.2 Metal Nanoclusters (m-NCs) and Photoluminescence
11.5 Synthesis of m-NPs and m-NCs
11.5.1 Biomineralization and Biomimetic Synthesis of NPs and NCs
11.5.2 Artificial Synthesis of m-NPs and m-NCs
11.6 Protein, Recombinant Protein, andPolypeptide-directed Synthesis of m-NPs and m-NCs
11.6.1 Biogenic Synthesis
11.6.2 Role of Amino Acids in the Process of the Formation and Structuring of Nanocrystals
11.6.3 Native Protein-directed Synthesis of m-NPs
11.6.4 Recombinant Protein and Polypeptide-directed Synthesis of m-NPs
11.6.5 Peptoid-directed Synthesis on m-NPs
11.6.6 Molecular Simulation and Structural Insights at Atomic-scale Resolution
11.7 Engineering Ultrasmall m-NCs
11.8 Applications of m-NPs and m-NCs
11.8.1 Antibacterial Activity against Multidrug-resistant Bacteria
11.8.2 Application of NPs and NCs as Sensors
11.8.3 Application of NPs and NCs in Catalysis
11.9 Conclusions and Perspectives
Dedication
Acknowledgements
References
Subject Index