This comprehensive overview of biomedical applications of vibrational spectroscopy focuses on methodologies that are most relevant to biodiagnostics. After a few introductory chapters that summarize the current status of the field, the reference covers current spectroscopic applications; new spectroscopic directions; and study design and the analysis of vibrational spectral fingerprints from complex biological and clinical samples . With chapters contributed by leading international experts, Biomedical Vibrational Spectroscopy is a core resource.
Author(s): Peter Lasch PhD, Janina Kneipp PhD
Edition: 1
Publisher: Wiley-Interscience
Year: 2008
Language: English
Pages: 403
City: Hoboken, N.J
BIOMEDICAL VIBRATIONAL SPECTROSCOPY......Page 3
CONTENTS......Page 7
Preface......Page 13
Contributors......Page 15
1 VIBRATIONAL SPECTROSCOPY IN MICROBIOLOGY AND MEDICAL DIAGNOSTICS......Page 19
1.1 Vibrational Spectra in Biomedicine Provide Fingerprint-like Signatures of Biological Structures......Page 20
1.2 Different Technical Options to Obtain the Spectral Information......Page 21
1.3 The Need for and Benefit from Data Evaluation......Page 22
1.4 Perspectives of Biomedical Vibrational Spectroscopy......Page 23
2.1 Introduction......Page 27
2.2 Measurement Techniques for Clinical Chemistry......Page 29
2.3 Measurement Techniques for Pathology......Page 37
2.4 Measurement Techniques for In Vivo Spectroscopy......Page 44
Acknowledgments......Page 49
References......Page 50
3.1 Introduction......Page 57
3.2 Specimen Sources, Experimental Schemes, and Optical Substrates......Page 59
3.3 Applications......Page 60
3.4 Instrumental Means of Biomedical IMS......Page 77
Acknowledgments......Page 89
References......Page 90
4.1 Introduction......Page 97
4.2 Vibrational Spectroscopy of Biofluids......Page 98
4.3 Quantification (Regression) and Diagnostic (Classification) Approaches......Page 99
4.4 Quantitative Biofluid Analysis......Page 100
4.5 Diagnostic Biofluid Tests......Page 106
4.6 Veterinary Applications......Page 110
4.7 Microfluidics and IR Spectroscopy of Biofluids......Page 113
4.8 Concluding Remarks......Page 117
References......Page 118
5.1 Introduction......Page 123
5.2 Background Fluorescence......Page 124
5.3 The Putative Drawback of a Low Signal-to-Noise-Ratio......Page 127
5.4 Spectroscopy of Blood and Its Derivates......Page 129
5.5 In Vitro Raman Spectroscopy of Serum for Laboratory Diagnostics: A Case Study......Page 130
5.6 Raman Spectroscopy of Body Fluids In Vivo......Page 133
5.7 Raman Spectroscopy of Other Body Fluids......Page 135
Acknowledgments......Page 136
References......Page 137
6.1 Introduction......Page 139
6.2 Infrared Histopathology: IR Microspectroscopic Mapping of Tissues......Page 140
6.3 Vibrational Cytology: IR and Raman Spectroscopy of Eukaryotic Cells......Page 151
6.4 Concluding Remarks......Page 165
References......Page 166
7.1 Hemoproteins......Page 171
7.2 Raman Microspectroscopy......Page 172
7.3 Outline of This Chapter......Page 173
7.4 Instrumentation and Spectral Data Analysis......Page 174
7.5 Resonance Raman Microspectroscopy on Neutrophilic Granulocytes......Page 177
7.6 Resonance Raman Microscopy on Neutrophilic Granulocytes......Page 183
7.7 Photobleaching and Light-Induced Cell Damage in Resonance Raman Microspectroscopy......Page 186
References......Page 190
8.1 Introduction......Page 199
8.2 Electronic Structure of Heme Moieties......Page 200
8.3 Resonance Raman Spectroscopy......Page 202
8.4 Resonance Raman Spectroscopy of Hemes in Cells and the Solid State......Page 205
8.5 Resonance Raman of Heme Derivatives Using Near-Infrared Excitation in the Solid State......Page 208
8.6 Application to Malaria Research......Page 215
References......Page 221
9.1 Introduction......Page 227
9.2 Theoretical Considerations......Page 228
9.3 CARS Microscopy......Page 230
9.4 Suppression of the Nonresonant Background......Page 231
9.5 Applications to Biology......Page 235
9.6 Outlook......Page 236
References......Page 237
10.1 Background......Page 239
10.2 Experimental Setup......Page 243
10.3 Results and Discussion......Page 246
Acknowledgments......Page 254
References......Page 255
11.1 Motivation: SERS and Cell Studies......Page 261
11.2 Probing Intrinsic Cellular Chemistry......Page 263
11.3 SERS-Based Optical Labels for Live Cell Studies......Page 271
11.4 Conclusions and Outlook......Page 274
References......Page 275
12.1 Introduction......Page 281
12.2 Optical Coherence Tomography......Page 284
12.3 Raman Spectroscopy of Mineralized Tissues......Page 291
12.4 Towards Clinical Dental Relevance......Page 299
Acknowledgments......Page 303
References......Page 304
13.1 Introduction......Page 309
13.2 AFMIR: Photothermal-Induced Resonance Experiment......Page 310
13.3 Experimental Illustration of the Photothermal Technique......Page 316
13.4 Applications: Biological Studies......Page 321
Acknowledgments......Page 329
References......Page 330
14 FROM STUDY DESIGN TO DATA ANALYSIS......Page 333
14.1 Aspects in the Design of Clinically Relevant Studies in Biomedical Vibrational Spectroscopy......Page 334
14.2 The Role of Noise and Reproducibility in the Raw Spectra......Page 339
14.3 Safeguarding the Analysis of Data and Its Interpretation......Page 341
14.4 Conclusion......Page 348
References......Page 349
15.1 Introduction to the Analysis of Several Data Sets......Page 351
15.2 Principal Component Analysis of One Data Table......Page 355
15.3 Simultaneous Analysis of Two Data Blocks by Partial Least-Squares Regression (PLSR)......Page 360
15.4 Simultaneous Analysis of Several Data Blocks by Multiblock PCA......Page 365
15.5 Alternative Multiblock Methods......Page 370
References......Page 372
16.1 Introduction......Page 375
16.2 IR Microscopic Characterization of an Unusual Form of Osteoporosis......Page 377
16.3 Applications to the Epidermis......Page 381
References......Page 394
INDEX......Page 397
