The Intemational Meeting on Vitamin B6 and Carbonyl Catalysis took place on Capri, Italy from 22nd to 27th May 1994 and was organized in conjunction with the 3rd Symposium on PQQ and Quinoproteins. It was an extraordinary occasion for scientists from all over the world to meet and discuss new developments in these overlapping fields. Several sessions were dedicated to the molecular aspects of Vitamin B6 and Quinone dependent enzymes, as well as to the cellular, biomedical and nutritional aspects. The congress was inaugurated by Paolo Fasella in his capacity as General Director of Science, Research and Development of the Commission of the European Communities, with an overview on Intemational Scientific Collaboration. The scientific sessions started with a talk on the History of Vitamin B6 given by David Metzler who at the very last minute presented Esmond Snell's paper adding some personal remarks. Unfortunately, both Esmond Snell and Alton Meister had to unexpectedly cancel the trip to Capri. These proceedings contain the papers presented as oral contributions and a few selected poster presentations. The limited number of pages meant we could not publish many interesting poster presentations, including those selected for the three lively and exciting evening poster discussion sessions called by the organizers "Vino, taralli and ... discussion".
Author(s): Esmond E. Snell (auth.), G. Marino, G. Sannia, F. Bossa (eds.)
Series: Advances in Life Sciences
Edition: 1
Publisher: Birkhäuser Basel
Year: 1994
Language: English
Pages: 366
Tags: Biochemistry, general; Pathology; Animal Physiology
Front Matter....Pages I-XVII
History of Vitamin B6....Pages 1-5
Front Matter....Pages 7-7
Molecular evolution of pyridoxal-5′-phosphate-dependent enzymes....Pages 9-13
Stereospecificity of aminotransferases for C-4′ hydrogen transfer and enzyme evolution....Pages 15-19
Detection of weak structural similarities among PLP dependent enzymes....Pages 21-25
Front Matter....Pages 27-27
Crystallographic studies on the vitamin B 6 -assisted enzymic transamination reaction....Pages 29-35
Redesign of aspartate aminotransferase specificity to that of tyrosine aminotransferase....Pages 37-41
Enzyme-substrate interactions modulating protonation and tautomerization states of the aldimines of pyridoxal enzymes....Pages 43-47
Functional role of the mobile stretch Gly36-Val37-Gly38 of porcine cytosolic aspartate aminotransferase....Pages 49-53
Using 1 H-and 15 N-NMR spectroscopy to observe active sites of porcine cytosolic and Escherichia coli aspartate aminotransferases....Pages 55-59
Aspartate aminotransferases like it hot....Pages 61-65
Studies on the mechanism of action of D-amino acid transaminase....Pages 67-73
Cytosolic factors and the twisting path from birth to berth in aspartate aminotransferases....Pages 75-80
Mitochondrial protein import: role of certain domains of mitochondrial aspartate aminotransferase....Pages 81-85
Cytosolic aspartate aminotransferase : tissue-specific hormonal regulation of a housekeeping gene promoter....Pages 87-92
Regulation by glucagon of serine:pyruvate/alanine:glyoxylate aminotransferase gene expression in cultured hepatocytes....Pages 93-97
Glutamate 1-semialdehyde aminotransferase, a unique enzyme in chlorophyll biosynthesis....Pages 99-103
Mechanism of glutamate semialdehyde aminotransferase probed with substrate analogues....Pages 105-109
Front Matter....Pages 111-111
Crystallographic and kinetic studies of the tryptophan synthase α 2 β 2 complex with a mutation in β subunit lysine-87 that binds pyridoxal phosphate....Pages 113-117
The Roles of Chemical Transformation, Loop Closure, Tunnel Function and Metal Ion Activation in the Tryptophan Synthase Mechanism....Pages 119-124
Effects of Monovalent Cations on Functional Properties of the Tryptophan Synthase α 2 β 2 Complex in Solution and in the Crystal....Pages 125-129
Front Matter....Pages 111-111
The long-range effect on the mutual activation of the α and β subunits in the α 2 β 2 complex of tryptophan synthase....Pages 131-133
X-ray Structure, Sequence and Solution Properties of Ornithine Decarboxylase from Lactobacillus 30a ....Pages 135-139
Dialkylglycine decarboxylase structure: alkali metal binding sites and bifunctional active site....Pages 141-145
The Location and Role of Active-Site Bases in PLP-Dependent Decarboxylase Enzymes as Deduced from Stereochemical and Kinetic Studies....Pages 147-151
GuCI-induced unfolding of pig kidney dopa decarboxylase: Evidence for a multi-state process....Pages 153-157
Front Matter....Pages 159-159
The transition state complex in the phosphorylase catalyzed reaction....Pages 161-166
Binding mode of glucans to α-glucan phosphorylase isozymes and their chimeric enzymes....Pages 167-171
Slow conformational transitions of muscle glycogen phosphorylase b ....Pages 173-180
Front Matter....Pages 181-181
X-ray study of tryptophanase at 2.1 Å resolution....Pages 183-185
Crystallographic studies of tyrosine phenol-lyase....Pages 187-191
Studies of the Mechanism of Tyrosine Phenol-lyase: Kinetics and Site-Directed Mutagenesis....Pages 193-197
The role of pyridoxal phosphate in the refolding of serine hydroxymethyltransferase....Pages 199-203
The Mechanism of O-Acetylserine Sulfhydrylase from Salmonella typhimurium ....Pages 205-209
Involvement of PLP in biological formation iron-sulfur clusters....Pages 211-215
Iron-sulfur clusters as alternatives to pyridoxal-5′-phosphate in bacterial L-serine dehydratases: Cloning of the gene encoding the β-subunit of the enzyme from Peptostreptococcus asaccharolyticus ....Pages 217-221
Phosphorescence of Pyridoxal Kinase at Room Temperature: The Effects of Ligands Bound to the Nucleotide Site....Pages 223-228
The Schiff base of pyridoxal-5′-phospate with ethylenediamine and ethylamine: Species in solution....Pages 229-231
Front Matter....Pages 233-233
Biological role and reactivity of TPQ containing amine oxidases....Pages 235-240
Two distinct quinoprotein amine oxidases are functioning in Aspergillus niger ....Pages 241-245
The catalytic competence of cobalt-amine oxidase....Pages 247-251
Front Matter....Pages 233-233
Mechanistic Studies of Copper/Topa Amine Oxidases....Pages 253-257
Design, synthesis and evaluation of mechanism-based inhibitors of copper amine oxidases....Pages 259-261
Copper ion-dependent biogenesis of topa quinone cofactor covalently bound to bacterial monoamine oxidase....Pages 263-267
Modulation of lysyl oxidase activity toward peptidyl lysine by vicinal dicarboxylic amino acid residues....Pages 269-273
Front Matter....Pages 275-275
The structure and function of quinoprotein dehydrogenases....Pages 277-281
Quinoprotein oxidoreductases for the oxidation of alcohols, sugars and amines....Pages 283-289
The ternary complex between methylamine dehydrogenase, amicyanin and cytochrome c 551i ....Pages 291-295
Model studies directed toward the action of quinoprotein methanol dehydrogenase....Pages 297-301
Front Matter....Pages 303-303
Nutritional aspects of vitamin B 6 : requirements, recommendations and intake....Pages 305-309
Vitamin B 6 transport and metabolism: Clues for delivery of bioactive compounds....Pages 311-317
Vitamin B 6 Modulation of Steroid Receptor-mediated Gene Expression....Pages 319-327
Front Matter....Pages 329-329
Pyridoxine (vitamin B 6 ), neurotransmitters and hypertension....Pages 331-335
A liver enzyme, serine:pyruvate/alanine:glyoxylate aminotransferase and its mutant in a primary hyperoxaluria type 1 case....Pages 337-341
Pyridoxal Phosphate, GABA and Seizure Susceptibility....Pages 343-347
Monoclonal antibodies to bovine brain GABA transaminase....Pages 349-351
Effect of pyrroloquinoline quinone (PQQ) on the SLE-like (Systemic Lupus Erythematosus) disease in MRL-Ipr/Ipr mice....Pages 353-357
Conclusions....Pages 359-360
Back Matter....Pages 361-366
