Since the first international meeting on Vitamin B6 involvement in catalysis took place in 1962, there have been periodic meetings every three or four years. In 1990, scientists studying another cofactor, PQQ, which had already attracted the scientific community's interest for its possible involvement in amino acid decarboxylation and reactions involving amino groups, joined forces with those investigating pyridoxal phosphate-dependent enzymes. Since then, the international PQQ/quinoproteins meetings have been held jointly. In the years following the original meeting 37 years ago in Rome, Italy, the scientific gatherings have taken place in Moscow, Russia (1966); Nagoya, Japan (1967); Leningrad (St. Petersburg), Russia (1974); Toronto, Canada (1979); Athens, Greece (1983); Turku, Finland (1987); Osaka, Japan (1990); and Capri, Italy (1996). For the first time in the history of these symposia, the international meeting was held in the United States, from October 31 through November 5, 1999, in Santa Fe, New Mexico. The scientific program focus shifted significantly beyond the original emphasis on catalysis to aspects such as cellular and genetic regulation of events involving proteins that require pyridoxal phosphate or quinoproteins. The growing awareness of the involvement of these proteins in biotechnology processes and fundamental physiological events, as well as their implication in diseases, was also represented, with emphasis on the molecular basis of these events. The meeting was symposium S278, sponsored by the International Union of Biochemistry and Molecular Biology (IUBMB).
Author(s): M. E. Winkler (auth.), Prof. Dr. Ana Iriarte, Prof. Dr. Marino Martinez-Carrion, Prof. Dr. Herbert M. Kagan (eds.)
Edition: 1
Publisher: Birkhäuser Basel
Year: 2000
Language: English
Pages: 377
Tags: Biochemistry, general; Biomedicine general; Molecular Medicine; Internal Medicine; Cell Biology
Front Matter....Pages I-XV
Front Matter....Pages 1-1
Genetic and Genomic Approaches for Delineating the Pathway of Pyridoxal 5’-Phosphate Coenzyme Biosynthesis in Escherichia coli ....Pages 3-10
Enzymes Catalysing Formation of Pyridoxal Phosphate From Vitamin B 6 ....Pages 11-15
A Divergence in the Biosynthetic Pathway and a New Role for Vitamin B6....Pages 17-22
Molecular Cloning and Catalytic Properties of Human Brain Pyridoxal Kinase....Pages 23-26
Front Matter....Pages 27-27
Regulation of the aspartate and alanine aminotransferases in humans and rodents....Pages 29-34
Mimosine’s Mechanism is Pyridoxal-Phosphate Independent....Pages 35-40
Environmental Stimuli and Regulatory Factors Affecting the Expression of the Glutamic Acid Decarboxylase System in Escherichia coli ....Pages 41-46
Front Matter....Pages 47-47
The membrane glucose dehydrogenase of Escherichia coli ....Pages 49-54
Structural properties of homodimeric quinoprotein ethanol deyhdrogenase from....Pages 55-60
Physiological Importance of Pyrroloquinoline Quinone....Pages 61-66
Mechanism of Topa Quinone Biogenesis in Copper Amine Oxidase Studied by Site-Directed Mutagenesis and X-Ray Crystallography....Pages 67-70
Lysyl Oxidase Activates the Transcription Activity of Collagen III Promoter: Possible Involvement of Ku Antigen....Pages 71-76
TGF-ß1 Regulation of Gingival Lysyl Oxidase and Connective Tissue Growth Factor....Pages 77-82
Pyridoxine, dopa decarboxylase, and tetrahydroisoquinoline derivatives in Parkinson’s disease....Pages 83-88
Front Matter....Pages 89-89
Lysyl oxidase: a family of multifunctional proteins....Pages 91-96
Structural Aspects of Lysyl Oxidase....Pages 97-100
Chemotaxis of Vascular Smooth Muscle Cells by Lysyl Oxidase....Pages 101-107
Front Matter....Pages 109-109
Biological Implications of the Different Hsp70 Binding Properties of Mitochondrial and Cytosolic Aspartate Aminotransferase.....Pages 111-116
Molecular Evolution of Alanine:Glyoxylate Aminotransferase Intracellular Targeting....Pages 117-122
Common Structural Elements in the Architecture of the Cofactor-Binding Domains in Unrelated Families of Pyridoxal Phosphate-Dependent Enzymes....Pages 123-128
Front Matter....Pages 109-109
Mitochondrial Localization of Eukaryotic NifS-Like Proteins....Pages 129-133
Mechanistic Studies Of 8-Amino-7-Oxononanoate Synthase.....Pages 135-142
Front Matter....Pages 143-143
Structure and Function of Tryptophan Synthase....Pages 145-150
Salt Bridging and Movalent Cation Binding Regulate Catalysis and Channeling in Tryptophan Synthase....Pages 151-156
Equilibrium Isotope Effects: Evidence for Low-Barrier H-Bonding in Tryptophanase....Pages 157-160
3-Dimensional Structures of Rabbit Cytosolic and E. Coli Serine Hydroxymethyltransferase....Pages 161-166
Role of Y65 and E57 in Escherichia coli Serine Hydroxymethyltransferase....Pages 167-170
Reaction and structure of 1-aminocyclopropane-1-carboxylate deaminase....Pages 171-174
Front Matter....Pages 175-175
Refined Reaction Mechanism of Aspartate Aminotransferase....Pages 177-182
1 H and 15 N NMR Spectroscopy of Aspartate Aminotransferase and Related Schiff Bases and Tautomerism in Enzyme Active Sites....Pages 183-188
Structure, Induced Fit and Substrate Recognition of E. coli Branched-Chain Amino Acid Aminotransferase....Pages 189-194
Front Matter....Pages 195-195
Tryptophan Tryptophylquinone Enzymes: Structure and Function....Pages 197-202
Continuous-Flow Column Electrolytic Spectroelectrochemical Method for Determination of Protein Redox Potentials — Application to Quinoproteins....Pages 203-208
Enantioselectivity of PQQ-containing Alcohol Dehydrogenases: Kinetic, Thermodynamic, and Molecular Modeling....Pages 209-212
Structural Studies of a soluble monomeric Quinohemoprotein Alcohol Dehydrogenase from Pseudomonas putida HK5....Pages 213-218
Electron Transport Systems for Quinohemoprotein Type II Alcohol Dehydrogenase of Pseudomonas Putida HK5....Pages 219-224
Front Matter....Pages 225-225
Activation and transformation of cells induce translocation of ornithine decarboxylase (ODC) to the surface membrane....Pages 227-232
A partially folded conformation is not the only requirement for import of mitochondrial aspartate aminotransferase....Pages 233-238
Engineering of B 6 enzymes....Pages 239-244
Association of Newly Synthesized Mitochondrial Aspartate Aminotransferase with Cytosolic Factors....Pages 245-249
Front Matter....Pages 225-225
Expression and Purification of Serine Palmitoyltransferase....Pages 251-254
Front Matter....Pages 255-255
5-Aminolevulinate Synthase: Pre-Steady State Reaction and Functional Role of Specific Active Site Residues....Pages 257-263
Function of Pyridoxal-5’-Phosphate in Bacterial Phosphorylases....Pages 265-270
Inhibition and Structural Changes of O -Acetylserine Sulfhydrylase-A from Salmonella typhimurium Upon Binding Sulfate and Chloride Anions....Pages 271-276
Structure-Function Relationships of Porcine Pyridoxal Kinase....Pages 277-280
The Contribution of a Conformationally-Mobile, Active-Site Loop to the Reaction Catalysed by Glutamate Semialdehyde Aminomutase....Pages 281-284
The Reactions of Glutamate 1-Semialdehyde Aminomutase with (R) and (S) Enantiomers of a Novel, Mechanism-Based Inhibitor, 2, 3-Diaminopropyl Sulfate....Pages 285-288
Structure and Function of E. Coli Pyridoxine Phosphate Oxidase....Pages 289-292
Front Matter....Pages 293-293
Role of Branched Chain Aminotransferase Isoenzymes in the Central Nervous System....Pages 295-300
Modulation of Gene Expression by Vitamin B 6 ....Pages 301-306
Pyridoxal 5-Phosphate and Calcium Channels....Pages 307-314
GABA-aminotransferase, a target for antiepileptic drug therapy....Pages 315-318
Front Matter....Pages 319-319
Mouse Ornithine Decarboxylase: Structural Comparisons to Other PLP-Dependent Enzymes....Pages 321-326
Structural and mechanistic studies of Trypanosoma brucei ornithine decarboxylase....Pages 327-332
Mechanistic Analysis of Dialkylglycine Decarboxylase....Pages 333-338
Studies on an Active Site residue, E177, That Affects Binding of the Coenzyme in D-Amino Acid Transaminase, and Mechanistic Studies on a Suicide Substrate....Pages 339-346
Front Matter....Pages 347-347
Functional properties of immobilized pyridoxal 5’-phosphate-dependent enzymes probed by absorption microspectrophotometry....Pages 349-354
Characterization of Recombinant Porcine Pyridoxal Kinase using Surface Plasmon Resonance Biosensor Technique....Pages 355-358
Three-Dimensional Model Of The ATP-Binding Domain Of Pyridoxal Kinase....Pages 359-362
Structural Fluctuations of Pyridoxal Kinase: Effect of Viscogen Cosolvents....Pages 363-368
Front Matter....Pages 347-347
The Aspartate Aminotransferase Folding Intermediates Recognized by GroEL are Partially Folded Monomers that Bind Pyridoxal Phosphate....Pages 369-373
Back Matter....Pages 375-377
