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Basic Lab Procedures in Clinical Bacteriology

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This manual is a practical guide, for use by laboratory workers in health centres and district hospitals, to the procedures to be followed in obtaining specimens, isolating and identifying bacteria, and assessing their resistance to antibiotics. It covers bacteriological investigation of blood, cerebrospinal fluid, urine, stool, sputum, pharyngeal and genital specimens, and purulent exudates. Particular attention is given to the need for quality control of all laboratory procedures. A list of media and reagents needed for the isolation and identification of the most common bacterial pathogens is included, together with an indication of their relative importance for the intermediary laboratory. This list is intended for adaptation to local circumstances.

This second edition has been updated in many areas, including a greatly enhanced section on stool specimens and a new section on serological tests.

Author(s): K. Engbaek, C.C. Heuck, P. Piot, P. Rohner, J. Vandepitte
Edition: Second Edition
Publisher: World Health Organization
Year: 2003

Language: English
Pages: 175

Cover......Page 1
Contents......Page 6
Introduction 1......Page 9
Quality assurance in bacteriology 2......Page 10
PART I Bacteriological investigations 19......Page 27
Blood 20......Page 28
Cerebrospinal fluid 25......Page 33
Urine 30......Page 38
Stool 37......Page 45
Upper respiratory tract infections 60......Page 68
Lower respiratory tract infections 66......Page 74
Sexually transmitted diseases 76......Page 84
Purulent exudates, wounds and abscesses 86 ......Page 95
Anaerobic bacteriology 98......Page 106
Antimicrobial susceptibility testing 103......Page 111
Serological tests 122......Page 130
PART II Essential media and reagents 141......Page 149
Introduction 142......Page 150
Pathogens, media and diagnostic reagents 143......Page 151
Selected further reading 154......Page 162
Index......Page 163