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Basic DNA N RNA Protocols

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Series: Methods in Molecular Biology
Publisher: Humana press

Language: English
Pages: 493

1 - The Simultaneous Isolationof RNA and DNAfrom Tissues and Cultured Cells......Page 1
2 - Restriction Endonuclease Digestionof DNA......Page 9
3 - Agarose Gel Electrophoresis......Page 15
4 - Capillary Blotting of Agarose Gels......Page 20
5 - Random Primed 32P-Labeling of DNA......Page 25
6 - Hybridization and CompetitionHybridization of Southern Blots......Page 29
7 - Utilization of DNA Probeswith Digoxigenin-Modified Nucleotidesin Southern Hybridizations......Page 39
8 - The Preparation of Fluorescein-LabeledNucleic Acid Probes and Their DetectionUsing Alkaline Phosphatase-CatalyzedDioxetane Chernilurninescence......Page 51
9 - Monitoring Incorporation of Fluoresceininto Nucleic Acid ProbesUsing a Rapid Labeling Assay......Page 63
10 - The Preparation of HorseradishPeroxidase-Labeled Nucleic Acid Probesand Their Detection Using EnhancedChemiluminescence......Page 65