In the face of rapid developments in automated techniques, this book presents an excellent guide to present trends. It provides information on: principles and terminology of enzyme automation; automatic methods illustrated by the Technicon system; semi-automatic methods; interrupted-flow and discrete-sampling systems; single-enzyme analysis; multiple-enzyme analysis (M.E.A.); enzyme characterization; calculation of enzyme activities from instrument readings; generalized systems for enzyme automation; Appendices I. Published automated enzyme assays, II. Terminology used in enzyme automation, III. Apparatus used in enzyme automation, IV. Computer program for generalized enzyme automated system.
Author(s): D.B. Roodyn (Eds.)
Series: Laboratory Techniques in Biochemistry and Molecular Biology 2
Publisher: Elsevier Science
Year: 1970
Language: English
Pages: ii-iii, 2-221
Content:
Editorial page
Page ii
Edited by
Page iii
Copyright page
Page 2
Preface
Pages 7-8
D.B. Roodyn
Introduction
Pages 9-12
Chapter 1 Principles and terminology of enzyme automation
Pages 13-25
Chapter 2 Semi-automatic methods
Pages 26-34
Chapter 3 Automatic methods illustrated by the Technicon system
Pages 35-58
Chapter 4 Interrupted-flow and discrete-sampling systems
Pages 59-67
Chapter 5 Single-enzyme analysis
Pages 68-86
Chapter 6 Multiple-enzyme analysis (M.E.A.)
Pages 87-101
Chapter 7 Enzyme characterization
Pages 102-113
Chapter 8 Calculation of enzyme activities from instrument readings
Pages 114-139
Chapter 9 Generalized system for enzyme automation
Pages 140-164
Conclusions and future prospects
Pages 165-167
Appendix I Published automated enzyme assays
Pages 168-172
Appendix II Terminology used in enzyme automation
Pages 173-175
Appendix III Apparatus used in enzyme automation
Pages 176-192
Appendix IV Computer program for generalized enzyme automation system
Pages 193-207
References
Pages 208-217
Subject Index
Pages 218-221
