Atomic Force Microscopy for Nanoscale Biophysics: From Single Molecules to Living Cells summarizes the applications of atomic force microscopy for the investigation of biomolecules and cells. The book discusses the methodology of AFM-based biomedical detection, diverse biological systems, and the combination of AFM with other complementary techniques. These state-of-the-art chapters empower researchers to address biological issues through the application of atomic force microscopy. Atomic force microscopy (AFM) is a unique, multifunctional tool for investigating the structures and properties of living biological systems under aqueous conditions with unprecedented spatiotemporal resolution.
Author(s): Mi Li
Publisher: Academic Press
Year: 2023
Language: English
Pages: 337
City: London
Front Cover
Atomic Force Microscopy for Nanoscale Biophysics
Copyright Page
Contents
About the author
Preface
1 Fundamentals and methods of atomic force microscopy for biophysics
1.1 Background of atomic force microscopy for biophysics
1.2 Atomic force microscopy topographical imaging modes
1.2.1 Basic principles
1.2.2 Contact mode
1.2.3 Noncontact mode
1.2.4 Tapping mode
1.2.5 Peak force tapping mode
1.3 Atomic force microscopy force spectroscopy techniques
1.3.1 Single-cell mechanical measurement
1.3.2 Single-cell force spectroscopy
1.3.3 Single-molecule force spectroscopy
1.4 High-speed atomic force microscopy
1.5 Topography and recognition imaging mode atomic force microcopy
References
2 Imaging and force detection of single deoxyribonucleic acid molecules by atomic force microscopy
2.1 Background
2.2 Sample preparation methods
2.3 Topographical imaging of single DNA molecules and events
2.4 Time-lapse imaging of individual DNA molecular dynamics
2.5 Extracting the persistence length of DNA molecules from atomic force microscopy images
2.6 Mechanically unzipping single DNA molecules by atomic force microscopy force spectroscopy
2.7 Probing individual DNA behaviors on DNA origami nanostructures
2.8 Summary
References
3 High-resolution imaging and force spectroscopy of single membrane proteins by atomic force microscopy
3.1 Background
3.2 Topographical imaging of single native membrane proteins
3.3 Unfolding mechanics of individual native membrane proteins
3.4 Observing the dynamics of single membrane proteins by high-speed atomic force microscopy
3.5 Multiparametric atomic force microscopy imaging of single membrane proteins
3.6 Topography and recognition imaging of single membrane proteins
3.7 Summary
References
4 Characterizing the nanostructures and mechanical properties of hydrogels by atomic force microscopy
4.1 Background
4.2 Nanostructures and nanomechanics of natural plant hydrogels
4.3 Characterizations of biopolymeric hydrogels inspired by carnivorous plant mucilage
4.4 Imaging and mechanical analysis of peptide-assembled nanofibrillar hydrogel
4.5 Probing the mechanical cues in cell–hydrogel interactions
4.6 Summary
References
5 Detecting the behaviors of single viruses by atomic force microscopy
5.1 Background
5.2 Imaging the fine structures of single viruses
5.3 Nanoindentation for mechanical measurements and manipulations of single viruses
5.4 Single-virus force spectroscopy for probing viral binding affinity
5.5 Multiparametric atomic force microscopy imaging of virus–cell interactions
5.6 Visualizing individual viral dynamics by high-speed atomic force microscopy
5.7 Summary
References
6 Imaging and mechanical analysis of single native exosomes by atomic force microscopy
6.1 Background
6.2 Exosome isolation and immobilization
6.3 Imaging single native exosomes in liquid
6.4 Measuring the mechanics of single native exosomes
6.5 Multiparametric imaging of single native exosomes
6.6 Single-molecule force spectroscopy on single exosomes
6.7 Summary
References
7 Nanoscale imaging and force probing of single microbial cells by atomic force microscopy
7.1 Background
7.2 Immobilization methods of living microbial cells for atomic force microscopy imaging
7.3 Visualizing the nanostructures and their dynamics of living microbial cells by atomic force microscopy
7.4 Measuring the mechanical properties of single living microbial cells by atomic force microscopy
7.5 Single-molecule force spectroscopy and single-cell force spectroscopy of microbial adhesion
7.6 Multiparametric atomic force microscopy imaging of single living microbial cells
7.7 Atomic force microscopy cantilever as a nanomechanical sensor for monitoring microbial activities
7.8 Summary
References
8 Investigating the structures and mechanics of single animal cells by atomic force microscopy
8.1 Background
8.2 Imaging the surface structures and their dynamics of single living adherent animal cells
8.3 Measuring the mechanical properties of single living adherent animal cells
8.4 Probing the molecular activities on the surface of single adherent cells
8.5 Visualizing the surface structures and their dynamics of single living suspended animal cells
8.6 Detecting the mechanical cues involved in the activities of lymphoma cells
8.7 Probing the molecular activities on the surface of primary lymphoma cells
8.8 Summary
References
9 Characterizing the extracellular matrix for regulating cell behaviors by atomic force microscopy
9.1 Background
9.2 Detecting the mechanical properties of decellularized extracellular matrix
9.3 Investigating the structures and mechanics of basement membranes
9.4 In situ imaging of cell culture medium-forming nanogranular surface for cell growth
9.5 Hierarchical micro-/nanotopography of extracellular matrix for tuning cellular structures and mechanics
9.6 Summary
References
10 Combining atomic force microscopy with complementary techniques for biophysics
10.1 Background
10.2 Scanning near-field ultrasound holography
10.3 Fluidic force microscopy
10.4 Combining atomic force microscopy with micropipette
10.5 Combining atomic force microscopy with fluidic environment
10.6 Summary
References
11 Future perspectives of atomic force microscopy for biophysics
References
Index
Back Cover
