Microarrays play an increasingly significant role in drug discovery. Written by a leader in the field, Applying Genomic and Proteomic Microarray Technology in Drug Discovery highlights, describes, and evaluates current scientific research using microarray technology in genomic and proteomic applications. The author addresses the drawbacks, helping you avoid unnecessary pitfalls, and provides practical tips on how to employ the technology in drug discovery and development. The book details the commercial landscape, covering the many issues surrounding the future adoption of gene expression and protein microarrays for pharmacogenomic and pharmacoproteomic applications. The author critically assesses those studies that have helped define applications in genomics and proteomics, explains gene expression microarray applications, and examines the utility of the protein microarray. He covers alternative substrates and the preparation of various surface chemistries together with their suitability for immobilization of nucleic acids and proteins. He delineates the mechanics of microarraying including environmental conditions, printer and pin performance, as well as discussion regarding setting up the print run. The book supplies protocols for printing nucleic acids and proteins and an in-depth discussion of other important parameters such as print buffers (inks) and factors influencing print quality. An understanding of the making of a microarray is fundamentally important to those interested in producing ''spotted'' arrays and their proper use. As this technology expands in popularity and usefulness, industry experts must grasp the fundamental principles behind it, its strengths, and its limitations. A basic reference for users of microarray technology in drug discovery, this book offers a detailed perspective and insight into the present and future uses of this technology.
Author(s): Robert S. Matson
Edition: 1
Publisher: CRC Press
Year: 2004
Language: English
Pages: 247
Applying Genomic And Proteomic Microarray Technology In Drug Discovery......Page 1
Preface......Page 4
Author......Page 6
Contents......Page 8
Introduction......Page 12
Content......Page 0
Terms and definitions......Page 13
Biomedical testing......Page 16
Biotechnology sector trends......Page 17
The Omics Era......Page 18
Role of gene expression microarrays in drug discovery......Page 22
Proteomics today: The great challenge......Page 25
Potential role for protein microarrays in drug discovery......Page 26
Stability and performance......Page 28
Detection......Page 30
Micro-ELISA formats......Page 31
Protein profiling formats......Page 32
Autoimmune diseases and allergies......Page 33
Future medicine: Pharmacoproteomics or pharmacogenomics?......Page 34
References......Page 37
In situ arrays......Page 41
Ex situ or spotted arrays......Page 48
3D and 4D chips......Page 54
Flow-through biochips......Page 55
Electronic biochips......Page 56
DNA microarrays......Page 60
Protein microarrays......Page 61
Tissue and cell microarrays......Page 62
References......Page 63
Substrates......Page 66
Use with nucleic acids......Page 68
Use with proteins......Page 69
Use with nucleic acids......Page 70
Use with proteins......Page 75
Use with nucleic acids......Page 77
Use with proteins......Page 79
Hydrogels......Page 80
Linkers......Page 82
Reactive groups......Page 92
Zammatteo et al. (2000): Preparation of carboxylic acid and aldehyde slides......Page 95
Benters et al. (2002): Preparation of carboxyl- and NHS-activated surfaces......Page 96
Summary......Page 97
References......Page 98
Introduction......Page 102
Substrates......Page 103
Probe composition (print buffer)......Page 104
Printing environment......Page 105
Printing mechanics......Page 106
Microarray pins......Page 113
Other approaches......Page 115
Printer performance......Page 121
Pin performance......Page 122
Microarray design......Page 124
Setting up a print run......Page 127
Printing parameters......Page 129
Preparing probe ink......Page 131
cDNA microarray......Page 133
Oligonucleotides......Page 134
Betaine......Page 136
Evaporation......Page 138
Print quality assessment......Page 141
Backgrounds......Page 145
Protocols for printing proteins......Page 147
Protocol of Haab et al. (2001)......Page 148
Protocol of MacBeath and Schreiber (2000)......Page 149
References......Page 153
Gene expression......Page 156
Drug discovery......Page 168
Drug toxicity......Page 170
Cancer......Page 173
Infectious disease......Page 185
Other disease states......Page 189
Bone pathology......Page 190
Multiple sclerosis......Page 191
References......Page 193
Spot theory......Page 196
Microtiter-based antibody arrays......Page 203
Membranes......Page 205
Glass slides......Page 208
Sensitivity and dynamic range......Page 216
mRNA–protein fusions......Page 224
Protein in situ array (PISA)......Page 226
Aptamers......Page 227
Universal protein array......Page 230
Phage display antibody selection......Page 236
Protein kinase microarray......Page 237
Second generation proteomics......Page 238
References......Page 241
Color Inserts......Page 246
