A collection of state-of-the-art molecular methods for studying antifungal resistance, for discovering and evaluating both new and existing antifungal drugs, and for understanding the host response and immunotherapy of such agents. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Antifungal Agents: Methods and Protocols offers clinician-scientists, microbiologists and molecular biologists the productive tools they need today to understand and successfully develop new therapeutic agents for yeast, mold, and fungal infections.
Author(s): Erika J. Ernst and P. David Rogers
Edition: 1
Publisher: Humana Press
Year: 2005
Language: English
Pages: 220
Cover......Page 1
Contents......Page 8
I INTRODUCTION......Page 12
1 Susceptibility Testing Methods of Antifungal Agents......Page 14
II ANTIFUNGAL RESISTANCE......Page 24
2 Deoxyribonucleic Acid Fingerprinting Methodsfor Candida Species......Page 26
3 CARE-2 Fingerprinting of Candida albicans Isolates......Page 38
4 Targeted Gene Deletion in Candida albicans Wild-TypeStrains by MPAR Flipping......Page 46
5 Application of Deoxyribonucleic Acid MicroarrayAnalysis to the Study of Azole Antifungal Resistancein Candida albicans......Page 56
6 Application of Proteomic Analysis to the Study of AzoleAntifungal Resistance in Candida albicans......Page 68
7 Techniques for Antifungal Susceptibility Testingof Candida albicans Biofilms......Page 82
III ANTIFUNGAL DEVELOPMENT AND EVALUATION......Page 92
8 Natural Products and Antifungal Drug Discovery......Page 94
9 Use of an Animal Model of Disseminated Candidiasisin the Evaluation of Antifungal Therapy......Page 122
10 Murine Model of Invasive Aspergillosis......Page 140
11 Antifungal Combinations......Page 154
12 Postantifungal Effect Methods......Page 164
IV HOST RESPONSES AND IMMUNOTHERAPY......Page 170
13 Evaluation of Immunotherapy in Invasive Candidiasis......Page 172
14 Interaction of Histoplasma capsulatum With HumanMacrophages, Dendritic Cells, and Neutrophils......Page 192
15 Immunology of Infection Caused by Cryptococcusneoformans......Page 204
Index......Page 210
