This book book is an attempt to review the recent progress in both electronics and computational tools developed to analyze the functional operations of large ensembles of neurons and to provide the readers with a sense of the applications made possible by these technological tools. While considerable progress has been made over the last decades in our understanding of electrophysiological processes at the single channel, single
synapse, and single neuron levels, our understanding of electrophysiological
processes at the neuronal network level is still in its infancy.
This book includes:
- Technological overviews of multi-electrode arrays (MEAs) and related electronics and software,
- Applications of MEAs to study ensemble activity of dissociated cell cultures including cardiac myocytes,
- Applications of the technology for drug discovery using acute and cultured slices.
Examples of studies directed at understanding the origins and functions of various rhythmic activities in neuronal ensembles.
Author(s): Jerome Pine (auth.), Makoto Taketani, Michel Baudry (eds.)
Edition: 1
Publisher: Springer US
Year: 2006
Language: English
Pages: 478
Tags: Neurosciences; Biomedical Engineering
Front Matter....Pages 1-1
A History of MEA Development....Pages 3-23
On Micro-Electrode Array Revival: Its Development, Sophistication of Recording, and Stimulation....Pages 24-37
Multi-Electrode Arrays: Enhancing Traditional Methods and Enabling Network Physiology....Pages 38-68
Development of 3-D Multi-Electrode Arrays for Use with Acute Tissue Slices....Pages 69-111
Electrophysiological Monitoring of Hippocampal Slice Cultures Using MEA on Porous Membrane....Pages 112-126
Mapping Spatio-Temporal Electrophysiological Activity in Hippocampal Slices with Conformal Planar Multi-Electrode Arrays....Pages 127-152
Pattern Technologies for Structuring Neuronal Networks on MEAs....Pages 153-189
Front Matter....Pages 191-191
Emerging Histiotypic Properties of Cultured Neuronal Networks....Pages 193-214
Closing the Loop: Stimulation Feedback Systems for Embodied MEA Cultures....Pages 215-242
Emerging Network Activity in Dissociated Cultures of Neocortex: Novel Electrophysiological Protocols and Mathematical Modeling....Pages 243-273
Analysis of Cardiac Myocyte Activity Dynamics with Micro-Electrode Arrays....Pages 274-290
Front Matter....Pages 291-291
A Hippocampal-Based Biosensor for Neurotoxins Detection and Classification Using a Novel Short-Term Plasticity Quantification Method....Pages 293-320
The Retinasensor: An In Vitro Tool to Study Drug Effects on Retinal Signaling....Pages 321-331
Chronic Alcohol Effects on Hippocampal Neuronal Networks....Pages 332-354
Applications of Multi-Electrode Array System in Drug Discovery Using Acute and Cultured Hippocampal Slices....Pages 355-376
Rhythm Generation in Spinal Cultures: Is It the Neuron or the Network?....Pages 377-408
Monitoring the Clock Neuron’s Tick: Circadian Rhythm Analysis Using a Multi-Electrode Array Dish....Pages 409-424
Investigation of Network Phenomena in Hippocampal Slices Using Multi-Electrode Recording Arrays....Pages 425-453
Exploring Fast Hippocampal Network Oscillations: Combining Multi-Electrode Recordings with Optical Imaging and Patch-Clamp Techniques....Pages 454-469
