This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a “fingerprint” for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.
Author(s): Peter Kapusta, Michael Wahl, Rainer Erdmann (eds.)
Series: Springer Series on Fluorescence 15
Edition: 1
Publisher: Springer International Publishing
Year: 2015
Language: English
Pages: 370
Tags: Spectroscopy/Spectrometry; Laser Technology, Photonics; Single Molecule Studies, Molecular Motors
Front Matter....Pages i-xii
Modern TCSPC Electronics: Principles and Acquisition Modes....Pages 1-21
Single-Photon Counting Detectors for the Visible Range Between 300 and 1,000 nm....Pages 23-42
Single-Photon Detectors for Infrared Wavelengths in the Range 1–1.7 μm....Pages 43-69
Modern Pulsed Diode Laser Sources for Time-Correlated Photon Counting....Pages 71-87
Advanced FCS: An Introduction to Fluorescence Lifetime Correlation Spectroscopy and Dual-Focus FCS....Pages 89-109
Lifetime-Weighted FCS and 2D FLCS: Advanced Application of Time-Tagged TCSPC....Pages 111-128
MFD-PIE and PIE-FI: Ways to Extract More Information with TCSPC....Pages 129-157
Photon Antibunching in Single Molecule Fluorescence Spectroscopy....Pages 159-190
FLIM Strategies for Intracellular Sensing....Pages 191-223
Multiple-Pulse Pumping with Time-Gated Detection for Enhanced Fluorescence Imaging in Cells and Tissue....Pages 225-239
Pattern-Based Linear Unmixing for Efficient and Reliable Analysis of Multicomponent TCSPC Data....Pages 241-263
Metal-Induced Energy Transfer....Pages 265-281
The Importance of Photon Arrival Times in STED Microscopy....Pages 283-301
Single-Color Centers in Diamond as Single-Photon Sources and Quantum Sensors....Pages 303-318
Photon Counting and Timing in Quantum Optics Experiments....Pages 319-341
Photon Counting in Diffuse Optical Imaging....Pages 343-365
Back Matter....Pages 367-370
