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Author(s): John W. Crabb (Eds.)
Series: Techniques in Protein Chemistry 6
Publisher: AP
Year: 1995

Language: English
Pages: 1-585

Content:
Foreword
Page xv
Joseph J. Villafranca

Preface
Page xvii
John W. Crabb

Acknowledgments
Page xix

The use of a volatile N-terminal degradation reagent for rapid, high-sensitivity sequence analysis of peptides by generation of sequence ladders Original Research Article
Pages 3-11
M. Bartlet-Jones, W.A. Jeffery, H.F. Hansen, D.J.C. Pappin

Investigation of polyethylene membranes as potential sample support for linking SDS-PAGE with MALDI-TOF MS for the mass measurement of proteins Original Research Article
Pages 13-19
James A. Blackledge, Anthony J. Alexander

Comparison of ESI-MS, LSIMS and MALDI-TOF-MS for the primary structure analysis of a monoclonal antibody Original Research Article
Pages 21-30
Leticia Cano, Kristine M. Swiderek, John E. Shively

MS based scanning methodologies applied to Conus venom Original Research Article
Pages 31-38
A. Grey Craig, Wolfgang H. Fischer, Jean E. Rivier, J. Michael McIntosh, William R. Gray

Direct coupling of an automated 2-dimensional microcolumn affinity chromatography-capillary HPLC system with mass spectrometry for biomolecule analysis Original Research Article
Pages 39-46
D.B. Kassel, T.G. Consler, M. Shalaby, P. Sekhri, N. Gordon, T. Nadler

Edman degradation and MALDI sequencing enables N- and C-terminal sequence analysis of peptides Original Research Article
Pages 47-54
Roland Kellner, Gert Talbo, Tony Houthaeve, Matthias Mann

Identification of the amino terminal peptide of N-terminally blocked proteins by differential deutero-acetylation using LC/MS techniques Original Research Article
Pages 55-62
Craig D. Thulin, Kenneth A. Walsh

HPAEC-PAD analysis of monoclonal antibody glycosylation Original Research Article
Pages 65-73
Jeffrey Rohrer, Jim Thayer, Nebojsa Avdalovic, Michael Weitzhandler

Carbohydrate structure characterization of two soluble forms of a ligand for the ECK receptor tyrosine kinase Original Research Article
Pages 75-82
Christi L. Clogston, Patricia L. Derby, Robert Toso, James D. Skrine, Ming Zhang, Vann Parker, G. Michael Fox, Timothy D. Bartley, Hsieng S. Lu

Characterisation of individual N-and O-linked glycosylation sites using Edman degradation Original Research Article
Pages 83-90
A.A. Gooley, N.H. Packer, A. Pisano, J.W. Redmond, K.L. Williams, A. Jones, M. Loughnan, P.F. Alewood

The unexpected presence of hydroxylysine in non-collagenous proteins Original Research Article
Pages 91-98
Michael S. Molony, Shiaw-Lin Wu, Lene K. Keyt, Reed J. Harris

Isolation of Escherichia coli synthesized recombinant proteins that contain ε-N-acetyllysine Original Research Article
Pages 99-106
Bernard N. Violand, Michael R. Schlittler, Cory Q. Lawson, James F. Kane, Ned R. Siegel, Christine E. Smith, Kevin L. Duffin

LC-MS methods for selective detection of posttranslational modifications in proteins: Glycosylation, phosphorylation, sulfation, and acylation Original Research Article
Pages 107-116
Mark F. Bean, Roland S. Annan, Mark E. Hemling, Mary Mentzer, Michael J. Huddleston, Steven A. Carr

Identification of phosphorylation sites by Edman degradation Original Research Article
Pages 117-123
John D. Shannon, Jay W. Fox

Determination of the disulfide bonds of human macrophage chemoattractant protein-1 using a gas phase sequencer Original Research Article
Pages 125-132
Ramnath Seetharam, Jeanne I. Corman, Shubhada M. Kamerkar

Enzymatic digestion of PVDF-bound proteins in the presence of glucopyranoside detergents: Applicability to mass spectrometry Original Research Article
Pages 135-142
Joseph Fernandez, Farzin Gharahdaghi, Sheenah M. Mische

In gel digestion of SDS PAGE-Separated proteins: Observations from internal sequencing of 25 proteins Original Research Article
Pages 143-152
Kenneth R. Williams, Kathryn L. Stone

Peptide mapping at the 1 μg level: In-gel vs. PVDF digestion techniques Original Research Article
Pages 153-160
Lee Anne Merewether, Christi L. Clogston, Scott D. Patterson, Hsieng S. Lu

Enzymatic digestion of proteins in zinc chloride and ponceau s stained gels Original Research Article
Pages 161-167
Sharleen Zhou, Arie Admon

Direct collection onto Zitex and PVDF for Edman sequencing: Elimination of polybrene Original Research Article
Pages 169-176
William A. Burkhart, Mary B. Moyer, Wanda M. Bodnar, Anita M. Everson, Violeta G. Valladares, Jerome M. Bailey

Minimizing N-to-O shift in Edman sequencing Original Research Article
Pages 177-184
William H. Vensel, George E. Tarr

The hydrolysis process and the quality of amino acid analysis: ABRF-94AAA collaborative trial Original Research Article
Pages 185-192
K. Ümit Yüksel, Thomas T. Andersen, Izydor Apostol, Jay W. Fox, Raymond J. Paxton, Daniel J. Strydom

A new reagent for cleaving at cystine residues Original Research Article
Pages 193-199
C. Mitchell, L. Hinman, L. Miller, P.C. Andrews

Protein sequence analysis using microbore PTH separations Original Research Article
Pages 201-208
Michael F. Rohde, Christi Clogston, Lee Anne Merewether, Patricia Derby, Kerry D. Nugent

Assignment of cysteine and tryptophan residues during protein sequencing: Results of ABRF-94SEQ Original Research Article
Pages 209-217
Jay Gambee, Philip C. Andrews, Karen DeJongh, Greg Grant, Barbara Merrill, Sheenah Mische, John Rush

Automated C-terminal protein sequence analysis using the hewlett-packard G1009A C-terminal protein sequencing system Original Research Article
Pages 219-227
Chad G. Miller, David H. Hawke, Jacqueline Tso, Sherrell Early

Applications using an alkylation method for Carboxy-terminal protein sequencing Original Research Article
Pages 229-237
MeriLisa Bozzini, Jindong Zhao, Pau-Miau Yuan, Doreen Ciolek, Yu-Ching Pan, John Horton, Daniel R. Marshak, Victoria L. Boyd

C-terminal sequence analysis of polypeptides containing C-terminal proline Original Research Article
Pages 239-247
Jerome M. Bailey, Oanh Tu, Gilbert Issai, John E. Shively

High sensitivity detection of tryptic digests using derivatization and fluorescence detection Original Research Article
Pages 251-258
Steven A. Cohen, Igor Mechnikov, Patricia Young

Reagents for rapid reduction of disulfide bonds in proteins Original Research Article
Pages 259-266
Rajeeva Singh, George M. Whitesides

Strategies for the removal of ionic and non-ionic detergents from protein and peptide mixtures for on- and off-line liquid chromatography mass spectrometry (LCMS) Original Research Article
Pages 267-275
Kristine M. Swiderek, Michael L. Klein, Stanley A. Hefta, John E. Shively

Online preparation of complex biological samples prior To analysis by HPLC, LC/MS and/or protein sequencing Original Research Article
Pages 277-284
Ken Stoney, Kerry Nugent

Methods for the purification and characterization of calcium-binding proteins from retina Original Research Article
Pages 285-292
Arthur S. Polans, Krzysztof Palczewski, Wojciech A. Gorczyca, John W. Crabb

Evidence for the presence of α-bungarotoxin in venom-derived κ-bungarotoxin Original Research Article
Pages 293-299
James J. Fiordalisi, Gregory A. Grant

Progress in the development of solvent and chromatography systems appropriate for bitopic membrane proteins Original Research Article
Pages 301-310
Song-Jae Kil, Lisa M. Oleksa, Geoffrey C. Landis, Charles R. Sanders II

Rapid separation of proteins and peptides using conventional silica-based supports: Identification of 2-D gel proteins following in-gel proteolysis Original Research Article
Pages 311-319
Robert L. Moritz, James Eddes, Hong Ji, Gavin E. Reid, Richard J. Simpson

Studying α-helix and β-sheet formation in small proteins Original Research Article
Pages 323-332
Catherine K. Smith, Mary Munson, Lynne Regan

Circular permutation of RNase T1 through PCR based site-directed mutagenesis Original Research Article
Pages 333-340
Jane M. Kuo, Leisha S. Mullins, James B. Garrett, Frank M. Raushel

E. coli-expressed human neurotrophin-3 characterization of a C-terminal extended product Original Research Article
Pages 341-348
John O. Hui, Shi-Yuan Meng, Vishwanatham Katta, Larry Tsai, Michael F. Rohde, Mitsuru Haniu

Spectral enhancement of recombinant proteins with tryptophan analogs: The soluble domain of human tissue factor Original Research Article
Pages 349-356
C.A. Hasselbacher, R. Rusinova, E. Rusinova, J.B. Alexander Ross

Utilization of partial reactions, side reactions, and chemical rescue to analyze site-directed mutants of ribulose 1,5-bisphosphate (RuBP) carboxylase/oxygenase (rubisco) Original Research Article
Pages 357-364
Mark R. Harpel, Engin H. Serpersu, Fred C. Hartman

Probing the roles of conserved histidine residues in β-galactosidase (E. coli) using site directed mutagenesis and transition state analog inhibition Original Research Article
Pages 365-371
Nathan J. Roth, Katherine Y.N. Wong, Reuben E. Huber

Rapid in vitro assembly of class I major histocompatibility complex Original Research Article
Pages 375-383
Nicholas J. Papadopoulos, James C. Sacchettini, Stanley G. Nathenson, Ruth Hogue Angeletti

Peptide models of bZIP proteins: Quantitative analysis of DNA affinity and specificity Original Research Article
Pages 385-391
Steven J. Metallo, Alanna Schepartz

Applying affinity coelectrophoresis to the study of non-specific, DNA binding peptides Original Research Article
Pages 393-400
Michael L. Nedved, Gregory R. Moe

Investigating calmodulin-target sequence interactions using mutant proteins and synthetic target peptides Original Research Article
Pages 401-408
Wendy A. Findlay, Stephen R. Martin, Peter M. Bayley

Interactions of bacterial cell-surface proteins with antibodies: a ersatile set of protein-protein interactions Original Research Article
Pages 409-416
Gordon C.K. Roberts, Lu-Yun Lian, Igor L. Barsukov, Jeremy P. Derrick, Koichi Kato, Yoji Arata

Studies of cytokine-cytokine receptor interactions: Influence of ligand dimerization Original Research Article
Pages 417-425
Larry D. Ward, Geoffrey J. Howlett, Robert L. Moritz, Annet Hammacher, Kiyoshi Yasukawa, Richard J. Simpson

New high sensitivity sedimentation methods: Application to the analysis of the assembly of bacteriophage P22 Original Research Article
Pages 427-432
Walter F. Stafford III, Sen Liu, Peter E. Prevelige Jr.

Cyanogen as a conformational probe Original Research Article
Pages 435-442
Richard A. Day, Amy Hignite, Warren E. Gooden

Evaluation of interactions between residues in α-helices by exhaustive conformational search Original Research Article
Pages 443-450
Trevor P. Creamer, Rajgopal Srinivasan, George D. Rose

Design, synthesis and characterization of a water-soluble β-sheet peptide Original Research Article
Pages 451-457
David S. Wishart, Les H. Kondejewski, Paul D. Semchuk, Cyril M. Kay, Robert S. Hodges, Brian D. Sykes

Automated analysis of protein folding Original Research Article
Pages 459-466
Richard A. Smith, Jack Henkin, Thomas F. Holzman

Hsp70-protein complexes: Their characterization by size-exclusion HPLC Original Research Article
Pages 467-474
Daniel R. Palleros, Li Shi, Katherine Reid, Anthony Fink

Methods for collecting and analyzing attenuated total reflectance FTIR spectra of proteins in solution Original Research Article
Pages 475-484
Keith A. Oberg, Anthony L. Fink

19F NMR studies of fluorinated sugars binding to the glucose and galactose receptor Original Research Article
Pages 487-494
Linda A. Luck

Heteronuclear gradient-enhanced NMR for the study of 20–30kDa proteins: Application to human carbonic anhydrase II Original Research Article
Pages 495-502
Ronald A. Venters, Leonard D. Spicer

Toward the solution structure of large (>30 kDa) proteins and macromolecular complexes Original Research Article
Pages 503-510
Cheryl H. Arrowsmith, Weontae Lee, Matthew Revington, Toshio Yamazaki, Lewis E. Kay

Solution structures of horse ferro- and ferricytochrome c using 2D and 3D 1H NMR and restrained simulated annealing Original Research Article
Pages 511-519
Phoebe X. Qi, Ernesto J. Fuentes, Robert A. Beckman, Deena L. Di Stefano, A. Joshua Wand

NMR relaxation methods to study ligand-receptor interactions Original Research Article
Pages 521-528
David W. Hoyt, Jian-Jun Wang, Brian D. Sykes

Application of 2-chlorotrityl resin: Simultaneous synthesis of peptides which differ in the C-termini Original Research Article
Pages 531-538
Anita L. Hong, Tin T. Le, Trung Phan

Correlation of cleavage techniques with side-reactions following solid-phase peptide synthesis Original Research Article
Pages 539-546
Gregg B. Fields, Ruth H. Angeletti, Lynda F. Bonewald, William T. Moore, Alan J. Smith, John T. Stults, Lynn C. Williams

Protein synthesis on a solid support using fragment condensation Original Research Article
Pages 547-554
Siegfried Brandtner, Christian Griesinger

Characterization of a side reaction using stepwise detection in peptide synthesis with Fmoc chemistry Original Research Article
Pages 555-562
Yan Yang, William V. Sweeney, Susanna Thörnqvist, Klaus Schneider, Brian T. Chait, James P. Tam

High sensitivity peptide sequence analysis using in Situ proteolysis on high retention PVDF membranes and a biphasic reaction column sequencer Original Research Article
Pages 565-574
Sandra Best, David F. Reim, Jacek Mozdzanowski, David W. Speicher

Index
Pages 575-585