A histone H3K36 chromatin switch coordinates DNA double-strand break repair pathway choice

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Figure™1Set2 is required to suppress HR and for resistance to DNA-damaging agents.(a) 10-fold serial dilutions of wild-type, set2Delta, set2-R255G and rad51Delta strains on YE6S, YE6S+5thinspmugthinspsolml bleomycin. Plates were incubated at 32thinspdegC......Page 2
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Figure™2Set2 is required for C-—NHEJ.(a) Serial dilutions of the indicated strains were spotted onto YE6S containing 5thinspmugthinspml-1 bleomycin or no drug. Plates were incubated at 32thinspdegC for 3 days. (b) Percentage survival of strains indicated......Page 4
Figure™3Gcn5 promotes HR and suppresses NHEJ.(a,b) Methylation and acetylation of H3K36 are mutually inhibitory. (a) Western blot analysis of H3K36me3 in wild type (Wt), set2Delta and gcn5Delta nuclear extract. (b) Western blot analysis of H3K36ac in wild......Page 5
Figure™4Histone H3K36 is required for survival following exposure to DSB-inducing agents.(a) Western blot analysis of H3K36me3 in wild-type (Wt) and H3.2K36R H3.1DeltaH3.3Delta (H3K36R) cells. alpha-H3 is shown as a loading control. (b) 10-fold serial dil......Page 6
Figure™5Chromatin is more accessible to MNase following DNA damage in set2Delta cells and less accessible in gcn5Delta cells.(a,b) Mid-log phase cells were incubated in 3thinspmugthinspml-1 bleocin for 1thinsph followed by MNase digestion for 10thinspmin......Page 7
Figure™6H3K36 methylation and acetylation are cell cycle regulated.(a) Flow cytometry analysis of wild-type cells following release from nitrogen starvation. The data shown were performed from at least two biological replicates. (b) Acid-extracted histone......Page 8
Figure™7An H3K36 chromatin switch regulates DSB repair pathway choice in fission yeast.In G1 high levels of Set2-dependent H3K36me (indicated) reduces nucleosome accessibility to repair factors, resulting in reduced resection and increased NHEJ repair of......Page 9
SymingtonL. S.GautierJ.Double-strand break end resection and repair pathway choiceAnnu. Rev. Genet.452472712011LieberM. R.The mechanism of double-strand DNA break repair by the nonhomologous DNA end-joining pathwayAnnu. Rev. Biochem.791812112010HeyerW. D......Page 10
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