This volume is a comprehensive collection of methods for the isolation, characterization, analysis and estimation of soluble lipoproteins. It surveys each aspect of lipoprotein technology in a critical manner which will enable the investigator to select the methods most relevant to his requirements. Having made his choice, he will then find a detailed description of how to perform the technique.
Author(s): R.H. BURDON and P.H. van KNIPPENBERG (Eds.)
Series: Laboratory Techniques in Biochemistry and Molecular Biology 14
Publisher: Elsevier Science Ltd
Year: 1984
Language: English
Pages: ii-viii, 1-512
Content:
Edited by
Pages ii-iii
Copyright page
Page iv
Preface
Pages v-vi
Glossary of lipoprotein nomenclature as used in this manual
Page vii
Glossary of apo-lipoprotein nomenclature as used in this manual
Page viii
Chapter 1 Brief introduction to the plasma lipoproteins
Pages 1-17
Chapter 2 The isolation and purification of plasma lipoproteins
Pages 18-116
Chapter 3 The characterisation of plasma lipoproteins: introduction to principles and methods
Pages 117-138
Chapter 4 Physical characterisation of lipoproteins
Pages 139-170
Chapter 5 Electrophoretic analysis of intact lipoproteins
Pages 171-220
Chapter 6 The chemical characterisation of lipoproteins
Pages 221-307
Chapter 7 The estimation of lipoproteins
Pages 308-383
Chapter 8 Isolation and assay of apo-lipoproteins
Pages 384-448
Appendix 1 The collection and preservation of blood plasma
Pages 449-459
Appendix 2 Methods of concentrating lipoprotein solutions
Pages 460-462
Appendix 3 The extraction of lipid from lipoproteins
Pages 463-471
Appendix 4 The preparation of stains
Pages 472-478
Appendix 5 The photography of electrophoresis and immuno-diffusion gels
Pages 479-481
Appendix 6 Radio-labelling
Pages 482-487
List of suppliers mentioned in the text
Pages 488-490
References
Pages 491-507
Subject index
Pages 508-512
