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Plasmids and Vectors Instructor Supplement to pGlo Bacterial Transformation A more detailed look at plasmids Cloning into a Plasmid Asilomar Conference Screening bacteria by replica plating Cosmids Other Vectors BACs (Bacterial artificial chromosomes) Large low copy number plasmids (have ori and ...
Date added: October 16, 2011 - Views: 187
Cosmids are extracted from bacteria ... rDNA Objectives Steps in rDNA Processing Genetic Engineering Advances The use of rDNA to Produce Human Insulin Cloning Vectors Plasmids Phage BACs and YACs HIV Gene Delivery System Summary References http://www.rvc.ac.uk/Extranet/DNA_1/DNA_1_intro ...
Date added: September 11, 2011 - Views: 255
cosmids Cosmids are plasmid vectors that contain one or two “cos sites”. (The cos site and the length between 2 cos sites is the only requirement for DNA to be
Date added: October 7, 2011 - Views: 204
Lambda vectors and their replication ... use a vector that can accept large fragments of DNA Vector types: 1. Plasmids- small circular DNA molecules which can replicate their DNA ... Cosmids- are hybrid vectors of l phage and plasmids. They can replicate their DNA in the cell with a ...
Date added: September 30, 2013 - Views: 28
Plasmids (Objectives of this Lecture) Understand the basic structures of plasmids Know the types of plasmids and their forms Undertand the methods used to transform E coli
Date added: May 3, 2013 - Views: 52
Cloning into Plasmids Restriction Fragment Cloning & PCR Cloning by the Topo TA™ Method Cloning Vectors The molecular analysis of DNA has been made possible by the cloning of DNA.
Date added: December 2, 2011 - Views: 89
Cosmids - Plasmids with phage packaging sites p. 2-16 4. YACs - Yeast Artificial Chromosomes p. 2-17 Vector Insert Size Vector Type ... you will be using plasmid vectors. p. 2-1 Plasmids Circular DNA molecules found in bacteria Replicated by the host’s machinery independently of the ...
Date added: November 12, 2011 - Views: 100
3 Basic Principles of Recombinant DNA Technology. ... DNA Cloning. V. Cloning Vectors. A. Bacterial Vectors. 1. Plasmids. 2. Bacteriophage. 3. Cosmids. B. Vectors for Other Organisms. 1. Yeast Artificial Chromosomes (YACs) 2. Bacterial Artificial Chromosomes (BACs) 3. Plant ...
Date added: May 26, 2012 - Views: 134
Methods in Molecular Biology and Genetic Engineering ... Vectors contain selectable markers and replication origins to allow identification and ... is a cloning vector 3.4 Cloning Vectors Can Be Specialized for Different Purposes Cloning vectors may be bacterial plasmids, phages, cosmids, ...
Date added: October 23, 2013 - Views: 5
Isolate Gene Fragmented Total DNA Use mRNA and Make DNA with Reverse Transcriptase Splice into Cloning Vehicle Plasmids ... Single Cleavage Site High copy Numbers Insertion Inactivation Virus Vectors Lambda Phage Vector Stable Packaging Cosmids Other Vectors Expression Vectors ...
Date added: May 25, 2013 - Views: 10
H4 Eukaryotic vectors — Yeast episomal plasmids Vectors for the cloning and expression of genes in Saccharomyces cerevisiae. Based on 2 micron (2m) ... H2 Bacteriophage vectors — Cloning in M13 H2 Bacteriophage vectors — Hybrid plasmid-M13 vectors H3 Cosmids, ...
Date added: February 13, 2012 - Views: 10
Plasmid vectors containing the lambda cos sites are called cosmids, ... Plasmids as Cloning Vectors Plasmids are useful cloning vectors (Figure 10.36) because they are easy to isolate and purify and can multiply to high copy numbers in bacterial cells.
Date added: May 29, 2013 - Views: 23
Plasmids and other types of cloning vectors were used by Human Genome Project researchers to copy genes and other pieces of chromosomes to generate enough ... Cosmids are artificially constructed cloning vectors that carry up to 45 kb of foreign DNA and can be packaged in lambda phage ...
Date added: August 27, 2013 - Views: 7
Lecture # 04 Cloning Vectors Recombinant DNA Technology Common General Cloning Strategy Target DNA from donor organism extracted, cut with compatible restriction endonuclease and ligated into a cloning vector.
Date added: February 18, 2014 - Views: 8
... General PowerPoint Presentation Types of Vectors and DNA delivery systems Types of Vectors Plasmids Plasmid ... Plasmid Placement in Cell PowerPoint Presentation Phage vectors Phage Insertion Cosmids YACs YACs YACs Essential elements for YACs PowerPoint Presentation ...
Date added: May 3, 2013 - Views: 22
Lecture 5 Recombinant DNA Technology Cloning Vectors Gene Libraries Clone Identification and Characterization Reading: Chapter 9 Molecular Biology syllabus web site
Date added: January 27, 2012 - Views: 18
Cosmids combine phage vectors’ ability to be packaged with the DNA propagation and purification properties of plasmids. ... to prevent degradation by cellular DNases.Picture Cosmid features and uses Cosmids are predominantly plasmids with a bacterial oriV, ...
Date added: May 21, 2012 - Views: 44
... F plasmid-based Cosmids: 5 kb plasmids, ... Overview of strategies for cloning genes * Mobilizing DNA: vectors for propagation in E. coli Plasmids Bacteriophage M13 Lambda Specialized cloning vectors expression vectors and tags vectors for large pieces of DNA, ...
Date added: November 15, 2011 - Views: 64
Molecular Cloning: Construction of a recombinant DNA Molecular Cloning: Cloning Vectors Plasmids Molecular Cloning: Cloning Vectors Plasmids Origin of replication, determines the number of copies per cell Marker genes: ampicillin and tetracycline resistance genes Unique restriction enzyme cut ...
Date added: May 6, 2013 - Views: 2
... Restriction enzymes - cleave at specific sites, palindromes, “sticky” or blunt ends after cleavage Ligation and vectors - plasmids, phage, cosmids, BACs, YACs (Table 4.2, p. 37) DNA libraries - genomic, cDNA Nucleic acid probes - cloned DNA or synthetic oligos “Instant” cloning ...
Date added: November 28, 2013 - Views: 3
... the site where DNA replication begins There are 2 major classes of vectors: Plasmids Phages Plasmids As ... -stranded phage DNA is secreted from transformed cells and can be collected from media Phagemids Phagemids are also vectors Like cosmids have aspects of both phages and plasmids ...
Date added: July 14, 2014 - Views: 4
Constructing Genomic and cDNA Libraries A. Definition A cloned set of rDNA ... 15-17kbp fragments of library Cosmids and YACs may also be used as vectors Contains at least one copy of all DNA fragments in the complete ... lambda insertion vectors or plasmids are used for ...
Date added: May 2, 2013 - Views: 21
... to separate the gene from the rest DNA 4.1 Gene Cloning The Role of Restriction Endonucleases Vectors Plasmids as Vectors Phages as Vectors λ ... λ Phage Vectors Cosmids M13 phage vectors ... cloning vectors are inducible, to avoid ...
Date added: January 28, 2012 - Views: 156
Cloning Vectors Enable DNA molecules to be replicated inside host ... Types of cloning vectors Plasmids: Autonomously replicating circular DNA molecules. 2. Bacteriophage: Small viruses that infect ... Examples: Cosmids PACs BACs Plasmid Vectors Origin of Replication b ...
Date added: May 13, 2013 - Views: 20
... the site where DNA replication begins There are 2 major classes of vectors: Plasmids Phages Plasmids As Vectors ... -stranded phage DNA is secreted from transformed cells and can be collected from media Phagemids Phagemids are also vectors Like cosmids have aspects of both phages and ...
Date added: July 10, 2014 - Views: 1
Other vectors include bacteriophage, cosmids, etc. pUC18 Plasmid Origin of replication DNA fragment up to 5 kbp can insert IV. Cloning with a Plasmid Vector Recombinant DNA Transformation Selection for cells carrying recombinant plasmids by plating cells on media with antibiotic.
Date added: January 13, 2013 - Views: 14
Recombinant DNA Technology Sherry ... replaced with 15-17kbp fragments of library Cosmids and YACs may also be used as vectors Contains at least one copy of all DNA fragments in the complete library Screened ... Bacteriophage lambda insertion vectors or plasmids are used for ...
Date added: October 7, 2011 - Views: 246
... genetic element used to propagate and express genes of interest in bacteria Plasmids, phages, cosmids, artificial chromosomes ... color change Plasmids vectors Easy to isolate and purify Can be introduced into bacteria by transformation Often bear antibiotic resistance genes that can ...
Date added: September 7, 2012 - Views: 21
Plasmids – used in cloning small segments of DNA (10-15 kb) Cosmids – plasmids containing DNA sequences (cos) from bacteriophage λ used to clone larger fragments of up to 45 Kb Bacteriophage λ ...
Date added: November 5, 2011 - Views: 77
... Plasmid cloning vector pBR322, Vectors for cloning large piece of DNA Bacteriophage-l and other phage vectors Cosmids phasmids Phagemids BAC vectors PAC vectors YAC vectors Model vectors for eukaryotes ... Phagemid Vectors Contain components from phage chromosomes and plasmids.
Date added: July 1, 2014 - Views: 3
... How to make and screen gene libraries How to make and screen cDNA libraries Several different cloning vectors including plasmids, bacteriophage lambda (l), and cosmids ...
Date added: October 6, 2011 - Views: 24
Types of cloning vectors 1. plasmids (about 20kb) 2. Bacteriophage (bacterial viruses), 30-50kb inserts 3. Cosmids (35-50kb insert) 4. BACs Use fertility F plasmid
Date added: May 2, 2013 - Views: 17
Larger DNA Fragments Can Be Cloned in Cosmids and Other Vectors Both λ phage vectors and the more commonly used E. coli plasmid vectors are useful for cloning DNA fragments up to ... recombinant plasmids containing inserted fragments up to 45 kb long can be efficiently introduced into E. coli ...
Date added: October 7, 2011 - Views: 250
Recombinant DNA Technology ... Plasmids: independently replicating DNA circles (only circles replicate in bacteria). Foreign DNA can be inserted into a plasmid ... Use these phage to infect new E. coli. Cosmids are similar to phage vectors: use lambda, but remove all but the ends (cos ...
Date added: October 7, 2011 - Views: 248
22. Size of the insert DNA is limited in plasmid cloning vectors, and plasmids carrying more than 5–10 kb are often unstable. 23. Larger inserts are needed for genomic analysis. Cosmids can accommodate inserts of 40–45 kb. Cosmids have an origin, selectable marker, and multiple cloning site.
Date added: August 6, 2014 - Views: 1
vectors: Plasmids; Phages . Selectable marker, Restriction sites for cloning. Origin of replication. pBR322. 4-pBR322 Cloning. Clone foreign DNA into . Pst. I site of pBR322. ... Cosmids. Cosmids. designed to clone large DNA fragments. Behave as plasmid and phage.
Date added: August 2, 2013 - Views: 13
... color change Plasmids vectors Easy to isolate and purify Can be introduced into bacteria by transformation Often bear antibiotic ... when foreign DNA is inserted Recombinant phage DNA can be packaged into viral capsids and used to infect a host cell Cosmids - plasmids with lambda ...
Date added: September 15, 2012 - Views: 18
Cosmids combination of lambda phage and plasmid hold inserts 35-45 kb FIGURE 20.6 3 ... Slide 5 Plasmids Cloning Vectors Plasmid Vector Other vectors Shuttle vectors Selection for E coli which contain plasmid Types of Genes No Lactose Present Lactose Present Transcriptional ...
Date added: March 14, 2012 - Views: 62
... Biochemistry Summary Figure after ... sequence Select Restriction Endonucleases Generating a Plasmid map restriction sites sizes when insert included Cloning Vectors 1. Plasmids- 5,000 to 400,000 bp useful for putting 0.01-10 ... Cosmids- artificially generated useful for putting 20-50 ...
Date added: May 2, 2013 - Views: 33
Definition of Recombinant DNA Production of a unique DNA molecule by joining together two or more DNA fragments not normally ... Cosmids , YACs, BACs ... vector Size of library depends on abundance of message Bacteriophage lambda insertion vectors or plasmids are often used for cloning cDNA ...
Date added: October 7, 2011 - Views: 105
Ligase: joins (seals) the ends of DNA Vectors: DNA molecule that can replicate & is used to transport foreign DNA Four types of Vectors: Plasmids; ... & Cosmids (plasmid + l cos site & can be pckged into phage capsid) Transgenic Plant: ...
Date added: August 2, 2013 - Views: 12
Unit I Lecture 4 B. Tech. (Biotechnology) III Year V th Semester EBT-501, Genetic Engineering BACs and PACs are vectors that can carry much larger fragments of DNA than cosmids because they do not have packaging constraints Figure 2.24 Genomes 3 (© Garland Science 2007) The PAC Mammalian ...
Date added: June 18, 2014 - Views: 4
Plasmid Vectors To replicate, a plasmid ... and DNA ligase joins them into one recombinant molecule. The recombinant plasmids are transformed into E ... I hope Cloning Vector Types For different sizes of DNA: plasmids: up to 5 kb phage lambda (λ) vectors (also cosmids): up to 50 kb BAC ...
Date added: May 8, 2012 - Views: 52
... specialized plasmids Vectors containing bacteriophage RNA polymerase promoters: for production of a specific RNA (probe synthesis, ... DNA molecules Cloning techniques Mobilizing DNA: vectors for propagation in E. coli Plasmids Bacteriophage M13 Lambda Cosmids and BACs a generic, ...
Date added: October 3, 2011 - Views: 89
... cannot replicate as phages but they are infectious so they carry their recombinant DNA into bacterial cells Cosmids M13 Phage Vectors Single-stranded DNA Site-directed mutagenesis ... Essential Features of Plasmids Most vectors currently in use carry a replicon from pMB1 or Col E1 ...
Date added: November 21, 2011 - Views: 20
... bacteria as the “factory” reproduce incredibly quickly have natural VECTOR Vectors carry the target gene Cloning Vectors segments of DNA that carry target gene; allow reproduction of gene plasmids phages cosmids part viral, ...
Date added: March 10, 2012 - Views: 20
Why Plasmids are Good Cloning Vectors small size (easy to manipulate and isolate) circular (more stable) replication independent of host cell several copies may be present ... Clone large inserts of DNA: size ~ 45 kb Features: Cosmids are Plasmids with one or two Lambda Cos sites.
Date added: June 1, 2013 - Views: 9