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Plasmids and Vectors Instructor Supplement to pGlo Bacterial Transformation A more detailed look at plasmids Cloning into a Plasmid Asilomar Conference Screening bacteria by replica plating Cosmids Other Vectors BACs (Bacterial artificial chromosomes) Large low copy number plasmids (have ori and ...
Date added: October 16, 2011 - Views: 186
Cosmids combine essential ... rDNA Objectives Steps in rDNA Processing Genetic Engineering Advances The use of rDNA to Produce Human Insulin Cloning Vectors Plasmids Phage BACs and YACs HIV Gene Delivery System Summary References http://www.rvc.ac.uk/Extranet/DNA_1/DNA_1_intro.htm ...
Date added: July 18, 2013 - Views: 31
cosmids Cosmids are plasmid vectors that contain one or two “cos sites”. (The cos site and the length between 2 cos sites is the only requirement for DNA to be
Date added: October 7, 2011 - Views: 201
Plasmids (Objectives of this Lecture) Understand the basic structures of plasmids Know the types of plasmids and their forms Undertand the methods used to transform E coli
Date added: May 3, 2013 - Views: 44
Solution :use a vector that can accept large fragments of DNA Vector types: 1. Plasmids- small circular DNA molecules which can replicate their DNA independently ... Cosmids- are hybrid vectors of l phage and plasmids. They can replicate their DNA in the cell with a plasmid and be packaged like ...
Date added: September 30, 2013 - Views: 27
Cosmids - Plasmids with phage packaging sites p. 2-16 4. YACs - Yeast Artificial Chromosomes p. 2-17 Vector Insert Size Vector Type ... you will be using plasmid vectors. p. 2-1 Plasmids Circular DNA molecules found in bacteria Replicated by the host’s machinery independently of the ...
Date added: November 12, 2011 - Views: 95
Cloning into Plasmids Restriction Fragment Cloning & PCR Cloning by the Topo TA™ Method Cloning Vectors The molecular analysis of DNA has been made possible by the cloning of DNA.
Date added: December 2, 2011 - Views: 83
Cosmids. B. Vectors for Other Organisms. 1. Yeast Artificial Chromosomes (YACs) 2. Bacterial Artificial Chromosomes (BACs) 3. Plant Cloning Vectors. 4. Mammalian ... Engineered Ti plasmids lack the tumor-causing genes, ...
Date added: May 26, 2012 - Views: 130
PLASMIDS PLASMID VECTORS Plasmid vectors are ≈1.2–3kb and contain: replication origin (ORI) sequence a gene that permits selection, ... than transformation for entry of chimeric DNA into E. coli host Maintain Cosmids as phage particles in solution But Cosmids are Plasmids: ...
Date added: January 23, 2013 - Views: 29
H4 Eukaryotic vectors — Yeast episomal plasmids Vectors for the cloning and expression of genes in Saccharomyces cerevisiae. Based on 2 micron (2m) ... H2 Bacteriophage vectors — Cloning in M13 H2 Bacteriophage vectors — Hybrid plasmid-M13 vectors H3 Cosmids, ...
Date added: February 13, 2012 - Views: 10
Cosmids combine phage vectors’ ability to be packaged with the DNA propagation and purification properties of plasmids. ... to prevent degradation by cellular DNases.Picture Cosmid features and uses Cosmids are predominantly plasmids with a bacterial oriV, ...
Date added: May 21, 2012 - Views: 42
The cosmid vector can carry up to 45 kb whereas plasmid and l phage vectors are limited to 25 kb. Cosmids are extracted from bacteria and mixed with restriction ... and it is forced to manufacture viral products, instead. COSMID VECTOR Cosmids are Plasmids with one or two Lambda Cos ...
Date added: March 19, 2014 - Views: 2
Commonly employed cloning vectors include plasmids and bacteriophages. ... Plasmid vectors containing the lambda cos sites are called cosmids, and they can carry a large fragment of foreign DNA.
Date added: May 29, 2013 - Views: 21
Lecture # 04 Cloning Vectors Recombinant DNA Technology Common General Cloning Strategy Target DNA from donor organism extracted, cut with compatible restriction endonuclease and ligated into a cloning vector.
Date added: February 18, 2014 - Views: 6
Plasmids and other types of cloning vectors were used by Human Genome Project researchers to copy genes and other pieces of chromosomes to generate enough identical ... Cosmids are artificially constructed cloning vectors that carry up to 45 kb of foreign DNA and can be packaged in lambda phage ...
Date added: August 27, 2013 - Views: 7
... General PowerPoint Presentation Types of Vectors and DNA delivery systems Types of Vectors Plasmids Plasmid ... Plasmid Placement in Cell PowerPoint Presentation Phage vectors Phage Insertion Cosmids YACs YACs YACs Essential elements for YACs PowerPoint Presentation ...
Date added: May 3, 2013 - Views: 20
... F plasmid-based Cosmids: 5 kb plasmids, ... Overview of strategies for cloning genes * Mobilizing DNA: vectors for propagation in E. coli Plasmids Bacteriophage M13 Lambda Specialized cloning vectors expression vectors and tags vectors for large pieces of DNA, ...
Date added: November 15, 2011 - Views: 60
Lecture 5 Recombinant DNA Technology Cloning Vectors Gene Libraries Clone Identification and Characterization Reading: Chapter 9 Molecular Biology syllabus web site
Date added: January 27, 2012 - Views: 14
Molecular Cloning: Construction of a recombinant DNA Molecular Cloning: Cloning Vectors Plasmids Molecular Cloning: Cloning Vectors Plasmids Origin of replication, determines the number of copies per cell Marker genes: ampicillin and tetracycline resistance genes Unique restriction enzyme cut ...
Date added: May 6, 2013 - Views: 2
Summary Restriction sites and enzymes Cloning vectors (plasmids) Inserting foreign genes in plasmids Hosts cells for replicating ... Types of vectors Bacterial plasmid bacteriophage cosmids bacterial artificial chromosome yeast artificial chromosome 6-12 25 35 300 200-1000 Maximum insert ...
Date added: October 10, 2012 - Views: 42
... the site where DNA replication begins There are 2 major classes of vectors: Plasmids Phages Plasmids As ... -stranded phage DNA is secreted from transformed cells and can be collected from media Phagemids Phagemids are also vectors Like cosmids have aspects of both phages and plasmids ...
Date added: July 14, 2014 - Views: 1
... bacteriophage lambda used as the vector “Stuffer fragment” removed and replaced with 15-17kbp fragments of library Cosmids and YACs may also be used as vectors Contains at least one ... A plasmid vector for cloning Plasmid vectors Examples pBR322 One of the original plasmids ...
Date added: May 2, 2013 - Views: 20
Plasmid vectors engineered from bacterial plasmids for use in cloning. Feeatures (e.g., E. coli plasmid vectors ... Fosmids offer higher stability than comparable high copy number cosmids. Contain other features similar to plasmids/cosmids such as origin sequence and polylinker ...
Date added: August 31, 2013 - Views: 3
... vector Size of library depends on abundance of message Bacteriophage lambda insertion vectors or plasmids ... bacteriophage lambda used as the vector “Stuffer fragment” removed and replaced with 15-17kbp fragments of library Cosmids and YACs may also be used as vectors Contains ...
Date added: October 7, 2011 - Views: 238
... the site where DNA replication begins There are 2 major classes of vectors: Plasmids Phages Plasmids As Vectors ... -stranded phage DNA is secreted from transformed cells and can be collected from media Phagemids Phagemids are also vectors Like cosmids have aspects of both phages and ...
Date added: July 10, 2014 - Views: 1
Summary Restriction sites and enzymes Cloning vectors (plasmids) Inserting foreign genes in plasmids Hosts ... Plating cells on agar surface to promote colony formation Cloning Types of vectors Bacterial plasmid bacteriophage cosmids bacterial artificial chromosome yeast artificial ...
Date added: September 27, 2011 - Views: 83
... the gene from the rest DNA 4.1 Gene Cloning The Role of Restriction Endonucleases Vectors Plasmids as Vectors Phages as Vectors λ Phage Vectors Cosmids M13 phage vectors Phagemids Eukaryotic Vectors Identifying a Specific Clone with a Specific Probe Polynucleotide ...
Date added: January 28, 2012 - Views: 150
Cloning Vectors Enable DNA molecules to be replicated inside host (e.g., bacteria) cells. ... Examples: Cosmids PACs BACs Plasmid Vectors Origin of Replication b-lactamase: breaks down ... Allows you to select bacteria that have plasmids with an insert.
Date added: May 13, 2013 - Views: 18
... but it can also be 125I Animations for two related uses of expression vectors Expression cloning of receptor ... How to make and screen gene libraries How to make and screen cDNA libraries Several different cloning vectors including plasmids, bacteriophage lambda (l), and cosmids ...
Date added: January 27, 2013 - Views: 12
... Plasmid cloning vector pBR322, Vectors for cloning large piece of DNA Bacteriophage-l and other phage vectors Cosmids phasmids Phagemids BAC vectors PAC vectors YAC vectors Model vectors for eukaryotes ... Phagemid Vectors Contain components from phage chromosomes and plasmids.
Date added: July 18, 2014 - Views: 1
Other vectors include bacteriophage, cosmids, etc. pUC18 Plasmid Origin of replication DNA fragment up to 5 kbp can insert IV. Cloning with a Plasmid Vector Recombinant DNA Transformation Selection for cells carrying recombinant plasmids by plating cells on media with antibiotic.
Date added: January 13, 2013 - Views: 12
... genetic element used to propagate and express genes of interest in bacteria Plasmids, phages, cosmids, artificial chromosomes ... color change Plasmids vectors Easy to isolate and purify Can be introduced into bacteria by transformation Often bear antibiotic resistance genes that can ...
Date added: September 7, 2012 - Views: 19
... How to make and screen gene libraries How to make and screen cDNA libraries Several different cloning vectors including plasmids, bacteriophage lambda (l), and cosmids ...
Date added: October 6, 2011 - Views: 20
Plasmids – used in cloning small segments of DNA (10-15 kb) Cosmids – plasmids containing DNA sequences (cos) from bacteriophage λ used to clone larger fragments of up to 45 Kb Bacteriophage λ ...
Date added: November 5, 2011 - Views: 75
... (methylated) at the corresponding sequences by a methylase. 2. Plasmids: independently replicating ... Plasmid Vectors To replicate, a plasmid ... I hope Cloning Vector Types For different sizes of DNA: plasmids: up to 5 kb phage lambda (λ) vectors (also cosmids): up to 50 kb BAC ...
Date added: May 8, 2012 - Views: 49
Types of cloning vectors 1. plasmids (about 20kb) 2. Bacteriophage (bacterial viruses), 30-50kb inserts 3. Cosmids (35-50kb insert) 4. BACs Use fertility F plasmid
Date added: May 2, 2013 - Views: 15
Plasmids for cloning carry drug resistance genes that are used for selection. Spread antibiotic resistance genes between bacterial species 3 ... Use these phage to infect new E. coli. Cosmids are similar to phage vectors: use lambda, but remove all but the ends (cos sites), ori, and selectable ...
Date added: October 7, 2011 - Views: 244
... color change Plasmids vectors Easy to isolate and purify Can be introduced into bacteria by transformation Often bear antibiotic ... when foreign DNA is inserted Recombinant phage DNA can be packaged into viral capsids and used to infect a host cell Cosmids - plasmids with lambda ...
Date added: September 15, 2012 - Views: 18
vectors: Plasmids; Phages . Selectable marker, Restriction sites for cloning. Origin of replication. pBR322. 4-pBR322 Cloning. Clone foreign DNA into . Pst. I site of pBR322. ... Cosmids. Cosmids. designed to clone large DNA fragments. Behave as plasmid and phage.
Date added: August 2, 2013 - Views: 9
Prokaryotes don’t glycosylate proteins Plasmids Plasmid- small, circular ... Cosmids- artificially ... Restriction Endonucleases Select Restriction Endonucleases Generating a Plasmid map Cloning Vectors Other Important Vectors Transformation of Bacteria CaCl2 Transformation ...
Date added: May 2, 2013 - Views: 29
Cosmids combination of lambda phage and plasmid hold inserts 35-45 kb FIGURE 20.6 3 ... Slide 5 Plasmids Cloning Vectors Plasmid Vector Other vectors Shuttle vectors Selection for E coli which contain plasmid Types of Genes No Lactose Present Lactose Present Transcriptional ...
Date added: March 14, 2012 - Views: 60
Larger DNA Fragments Can Be Cloned in Cosmids and Other Vectors Both λ phage vectors and the more commonly used E. coli plasmid vectors are useful for cloning DNA fragments up to ... recombinant plasmids containing inserted fragments up to 45 kb long can be efficiently introduced into E. coli ...
Date added: October 7, 2011 - Views: 245
... Cosmids, YACs, BACs ... vector Size of library depends on abundance of message Bacteriophage lambda insertion vectors or plasmids are often used for cloning cDNA libraries The choice depends upon: ...
Date added: October 7, 2011 - Views: 102
... Vectors and Creating Recombinant DNA Recombinant DNA Cloning Vectors Cloning Vectors and Creating Recombinant DNA Plasmids as Cloning Vectors ... Construction of a Recombinant Plasmid Phage Vectors Cosmids Artificial Chromosomes Artificial Chromosomes as Cloning Vectors ...
Date added: June 24, 2013 - Views: 3
Why Plasmids are Good Cloning Vectors small size (easy to manipulate and isolate) circular (more stable) replication independent of host cell several copies may be present ... Clone large inserts of DNA: size ~ 45 kb Features: Cosmids are Plasmids with one or two Lambda Cos sites.
Date added: June 1, 2013 - Views: 9
Ligase: joins (seals) the ends of DNA Vectors: DNA molecule that can replicate & is used to transport foreign DNA Four types of Vectors: Plasmids; ... & Cosmids (plasmid + l cos site & can be pckged into phage capsid) Transgenic Plant: ...
Date added: August 2, 2013 - Views: 12
... cannot replicate as phages but they are infectious so they carry their recombinant DNA into bacterial cells Cosmids M13 Phage Vectors Single-stranded DNA Site-directed mutagenesis ... Essential Features of Plasmids Most vectors currently in use carry a replicon from pMB1 or Col E1 ...
Date added: November 21, 2011 - Views: 16
Unit I Lecture 4 B. Tech. (Biotechnology) III Year V th Semester EBT-501, Genetic Engineering BACs and PACs are vectors that can carry much larger fragments of DNA than cosmids because they do not have packaging constraints Figure 2.24 Genomes 3 (© Garland Science 2007) The PAC Mammalian ...
Date added: June 18, 2014 - Views: 2
Cloning Vectors Target Gene ... encoded by the Protein X gene Define the Protein X gene structure Construct expression plasmids for functional studies of Protein X in cells Mutagenize the ... Bacterial host. Insert size range < 15kb. Bacteriophage-based Cosmids: Linear DNA molecules ...
Date added: October 7, 2011 - Views: 103
... specialized plasmids Vectors containing bacteriophage RNA polymerase promoters: for production of a specific RNA (probe synthesis, ... DNA molecules Cloning techniques Mobilizing DNA: vectors for propagation in E. coli Plasmids Bacteriophage M13 Lambda Cosmids and BACs a generic, ...
Date added: October 3, 2011 - Views: 86