Nanotools Lc3 PDF
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proteins only LC3 (microtubule-associated protein1 light chain 3) is expressed at sufficient high ... www.nanotools.de Background and Specificity: Related Products Immunoprecipitation: ND Immunoblotting: 0.5 µg/ml for HRPO/ECL detection
polyclonal antibody (Rockland Immunochemicals); anti-LC3 monoclonal antibody (NanoTools Antikorpertechnik); anti-actin and anti-LAMP1 monoclonal antibodies (Sigma); and ... LC3, and ubiquitinated proteins are degraded by autophagy. Both large (more than 1 m in diameter) and small (less than
Nanotools (LC3 and 5F10), ASPP1 (LX54.2), ASPP2 (DX54.10) (5), iASPP (LX49.3), and Novocastra (p53 CM-5). Signals were detected using an ECL detection system (GE Healthcare). Autophagic Activity Assays. Autophagy was induced by several
Signaling)(1:700);LC3(NanoTools)(0.5 g/ml);AKT(Cell Signaling) (1:2000); phospho-AKT (Ser-473) (1:1000); Sirt1 (Millipore) (1:1000). GAPDH (Santa Cruz Biotechnology) ... LC3 adenovirus were analyzed for induction of autophagy in fed and starved conditions.
3.1.15 Mouse Monoclonal Antibody anti-LC3 antibody (NanoTools, 0231-100/LC3-5F10) 3.1.16 Peroxidase-conjugated AfiniPure Donkey Anti-Mouse IgG (Jackson ImmunoResearch Labs, 715-035-151) 3.1.17 ECL Western Blotting Substrate (Pierce, 32106) 3.1.18 ...
19Jia Wang様.mcd Page 4 09/10/30 18:53 v4.21 (M0887,Dako),Beclin-1(Sc-11427,SantaCruz,SantaCruz, CA, USA)27 and LC3 (0231s0104, Nanotools, Teningen,
173 Santa Cruz, CA) (1:500 dilution), mouse anti-LC3 (NanoTools, Teningen, Germany) 174 (1:500 dilution) or rabbit anti-GAPDH (Sigma) (1:10,000 dilution) followed by 175 extensive washing and incubation for 1 h with the appropriate horseradish
Comparative utility of LC3, p62 and TDP-43 immunohistochemistry in differentiation of inclusion body myositis from polymyositis and ... Nanotools; 1:100 dilution following antigen retrieval) and p62/SQSTM1 (guinea pig polyclonal antibody, Progen Biotechnik; 1:100 dilution following anti-
Beclin 1 Is Required for Starvation-Enhanced, but Not Rapamycin-Enhanced, LC3-Associated Phagocytosis of Burkholderia pseudomallei in RAW 264.7 Cells
AnbodiesusedweretheMitoProﬁleTotalOXPHOSkit(Mitosciences/Abcam),LC3(NanoTools)andacn(Sigma).AnalysiswasdonebynormalizingintegratedintensiestoacnlevelsusingtheOdysseysoware.StascalanalysiswasdoneusingGraphPad(Prism). ... LC3(green)andDAPI(blue).(C)Cellswerestainedwiththeautophagy
LC3 (nanoTools) or PM036 (MBL), anti-BECLIN1 (BD Biosciences), anti- ... LC3-II during the biphasic LC3-II increase (Fig. 2A), suggesting that TGF-β–mediated autophagy activation is sustained fora longer pe-riod after the early time-course activation.
The following primary antibodies were used: anti-LC3 (clone 5F10; Nanotools for immunofluorescence assay and MBL International for western blot) and antitubulin (Sigma). Antirabbit or antimouse horseradish peroxidase (HRP) conjugated secondary antibodies (Jackson
and stained with 4, 6-diamidinophenylindole (DAPI) and anti-LC3 antibody from either Nanotools (France) or Abcam. Stained cells were visualized under a confocal microscope attached to an IX70 inverted microscope (Olympus, Melville, NY).
nologies, Danvers, MA), and anti-mouse LC3 (Nanotools). Plates were washed with 1 PBS three times, and secondary antibody was applied, either horseradish peroxidase-conju-gatedhorseanti-rabbitSuperPicture(ZymedLaboratoriesInc.,
The following antibodies were used for immunohistochemistry: LC3 (NanoTools), Dec1 (Giatromanolaki et al. 2003) and Ki67 (Neomarkers). The following antibodies were used for immunofluorescence; ATG12 (Cell Signaling) and HA (clone 12CA5; Cancer Research UK in house).
1/100) from NanoTools; LC3 (polyclonal, 1/100) from MBL; slow type I MyHC (NCL-MHCS, monoclonal, 1/20), all fast type II MyHC (NCL-MHCf, monoclonal, 1/20) from Novocastra; BrdU (monoclonal, 1/200) and MEF2D (monoclonal, 1/2500) antibod-
microtubule-associated protein 1 light chain 3 (LC3 or Atg8) by Atg4 releasing the soluble form LC3-I, which is then conjugated to phosphatidylethanolamine (PE)
antibody for the autophagy marker, LC3 (LC3-5F10; 30 g/mouse; NanoTools, San Diego, CA, USA) was injected into 3 mice in each of the 4 experimental groups to label the autophagic osteocytes. All animals were treated accord-
1:1000), mouse anti-LC3 (Nanotools; 1:100), mouse anti-PML (Santa Cruz ... and LC3-positive cytoplasmic dots inATRAandATO–treated cells compared with untreated cells (Figure 3C-D). We also performed immunoﬂuorescence analysis of untreated and drug-treated cells by
dependent on LC3 abundance, suggesting that TBC1D5 can switch from retromer-decorated endosomal to LC3-lipidated ... (NanoTools), rabbit polyclonal anti-LC3 (MBL) and rabbit polyclonal anti-LC3B (a gift from Z. Elazar), rabbit polyclonal anti-p62 (Enzo Life
(Thr389) antibodies (Cell Signaling Technologies); monoclonal anti-LC3 antibody(nanoTools,thoughAxxora;LLC). siRNApreparationandtransfection.siRNAsequencetargetingeEF-2 kinase mRNA corresponded to the coding region 144 to 164 (5¶-
mouse α-LC3 (1:1000, NanoTools 0260), rabbit α-β-tubulin (1:20000, Santa Cruz, sc-9104), ... LC3-II levels due to the accumulation of undigested autophagosomes. p62 is a protein degraded by au-tophagy and commonly monitored as a marker of
Transient DsRed–LC3 and green fluorescent protein–Endo transfection. PCR-amplified coding sequences of LC3 in green fluorescent protein (GFP) ... (1:1000; TUJ-1; R&D Systems), overnight for LC3 (1:250; Nanotools) and cathepsin D (1:5000; in house) at 4°C. Cells were washed (three
clonal anti–LC3 antibody (1:100; Nanotools Antik€orper-technik GmbH & Co Antik€orpertechnik GmbH & Co; clone 5F10) as primary antibodies. Incubation with Alexa-488–conjugated secondary antibody (Invitrogen) was done in the dark at room temperature for 1 hour.
Adescribedα-LC3 rabbitpolyclonalsera was used for immunoblotting (5). ... 5F10;NanoTools/Axorra)for1hatroomtemperature.Secondary antibody (OmniMap anti-Ms HRP; Ventana) was applied for 12 min, and ChromoMap DAB (Ventana) was used for detection.
anti-LC3, and anti- -tubulin ... (Cambridge, MA), nanoTools (Teningen, Germany), and Sigma-Aldrich, respectively. Anti-WIPI-1 antiserum was described previously (Proikas-Cezanne et al., 2004). Anti-rabbit IgG-Alexa Fluor 546 and TO-PRO-3 was obtained from Invitrogen.
Signaling Technology, Danvers, MA), LC3 (Nanotools, Teningen, Germany), and LRRK2 (C41-2; Michael J. Fox Foundation, New York City, NY). Calcium Imaging ... LC3 suppresses neurite shortening caused by mutant LRRK2.12,19 However, the substrate(s) removed by auto-
Biotechnology); and anti-LC3 (Nanotools, Munich, Germany). Immunohistochemistry Immunohistochemical analyses were performed as described previ-ously.14,18 Primary antibodies were purchased as follows: anti-HA, anti-Lamp1, and anti-cathepsin D (Santa Cruz Biotechnology);
The primary antibodies used for immunocytochemistry included mouse anti-LC3 (1:100, Nanotools, Teningen, Germany), Rabbit anti-RhoB (1:100, Santa Cruz), Rabbit anti 20S core (1:100, Biomol) or p62/SQSTM1 (1:250 Biomol). siRNAs applied
Mouse anti-LC3 antibody (NanoTools, Antikörpertechnik GmbH & Co, Teningen, Germany). 2. pBABE-GFP-LC3 vector (Dr. J. Debnath, Dept. Cell Biol- ... LC3-I is highly susceptible to degradation in the lysis buffer, especially during freeze–thaw cycles.
clonal antibody to LC3 (1:1200; Nanotools). Secondary antibodies were HRP-conjugated goat anti-mouse, goat anti-rabbit IgG (1:10,000; Pro- ... transfectedGFP–LC3,amarkerproteinofautophagosomes,with orwithoutBafA1treatment(Fig.2C),confirmingthatautophagy
Nanotools の抗体と両方 ... TP53INP1, a tumor suppressor, interacts with LC3 and ATG8 family proteins through the LC3interacting region (LIR) and promotes autophagydependent cell death 22421968 2012 Cell Death Differ in press IC Human, Mouse U2OS, HeLa.
77 (Lorrach, Germany); anti-LC3 (clone 5F10) from NanoTools (Teningen, Germany); and 78 anti-β-actin from Sigma-Aldrich. Secondary antibodies HRP donkey anti-rabbit and 79 sheep anti-mouse were purchased from GE Healthcare (Bucks, UK). 80 81 ...
Mouse anti-LC3 antibody (NanoTools, Antikörpertechnik GmbH&Co,Teningen,Germany). 2. pBABE-GFP-LC3vector(Dr.J.Debnath,Dept.CellBiol- ... LC3-I and a simultaneous conversion to LC3-II, suggesting the formation of autophago-somes after PDT. (b) ...
1:1000), mouse anti-LC3 (Nanotools; 1:100), mouse anti-PML (Santa Cruz Biotechnology; 1:200), rabbit anti-PML(Santa Cruz Biotechnology; 1:200), and mouse anti–CD11b ﬂuorescein isothiocyanate (FITC; Abcam; 1:50). Reagents
147 ß-actin was from Sigma (Munich, Germany), mouse-monoclonal anti-LC3 antibody was 148 obtained from nanoTools Antikörpertechnik GmbH & Co., KG (Teningen, Germany) and the 149 mouse monoclonal anti-ACE2 antibody was obtained from Enzo Life Science (Lörrach,
Anti-LC3 (clone 2G6) and anti-p42 ERK (clone 3A7) monoclonal antibodies were obtained from Nanotools and Cell Signaling Technology, respectively. Anti–Bcl-2 and anti-CrmA antibodies were from BD Biosciences, and anti–Bcl-X L was purchased from Santa
Material y métodos - 174 - Belgium). El anticuerpo frente LC3 (clone 5F10) es de Nanotools (Teningen, Germany), el anticuerpo frente al TGN46 humano de Serotec (Oxford, UK) y el anticuerpo ATP6J frente a