The aim of molecular diagnostics is preferentially to detect a developing disease before any symptoms appear. There has been a significant increase, fueled by technologies from the human genome project, in the availability of nucleic acid sequence information for all living organisms including bacteria and viruses. When combined with a different type of instrumentation applied, the resulting diagnostics is specific and sensitive. Nucleic acid-based medical diagnosis detects specific DNAs or RNAs from the infecting organism or virus and a specific gene or the expression of a gene associated with a disease. Nucleic acid approaches also stimulate a basic science by opening lines of inquiry that will lead to greater understanding of the molecules at the center of life. One can follow Richard Feynman’s famous statement “What I cannot create, I do not understand.”
Author(s): Volker A. Erdmann, Stefan Jurga, Jan Barciszewski (eds.)
Series: RNA Technologies
Edition: 1
Publisher: Springer International Publishing
Year: 2015
Language: English
Pages: 349
Tags: Human Genetics; Nucleic Acid Chemistry; Molecular Medicine; Biotechnology
Front Matter....Pages i-x
Electrochemical Biosensors for miRNA Detection....Pages 1-19
Electrochemical Detection of RNA....Pages 21-45
DNA and PNA Probes for DNA Detection in Electroanalytical Systems....Pages 47-80
DNA for Non-nucleic Acid Sensing....Pages 81-106
Aptamers in Oncotherapy....Pages 107-121
Genotyping of Single Nucleotide Polymorphisms....Pages 123-144
Environmentally Responsive and Bright Fluorescent Probes Possessing Dansyl-Modified Oligonucleotides Under Hybridization of DNA and RNA....Pages 145-159
Fluorescent Nucleic Acid Analogues in Research and Clinical Diagnostics....Pages 161-181
Emergent Properties and Functions of Nanoconfined Nucleic Acid Architectures....Pages 183-204
RNA and DNA Diagnostics on Microspheres: Current and Emerging Methods....Pages 205-224
DNA and RNA Electronic Properties for Molecular Modifications and Environmental State Diagnostics....Pages 225-239
Mechanochemical Sensing....Pages 241-258
Microarrays as Research Tools and Diagnostic Devices....Pages 259-280
Quadruplex Priming Amplification (QPA) for Nucleic Acid Diagnostics....Pages 281-295
Single-Molecule Strategies for DNA and RNA Diagnostics....Pages 297-332
Detection and Assessment of MicroRNA Expression in Human Disease....Pages 333-349
