This book discusses the aspects of protein and peptide-based microarrays for multiplex detection, based on Rabiee's Theory. It provides a thorough introduction to the field, before examining protein and peptide-based microarrays in greater detail, along with their related problems and solutions. Also covered are the materials and fabrication methods involved with protein microarrays. It offers an outlook on the future of the field within its conclusion and provides an understanding of cutting-edge aspects and horizons of this topic and is an excellent guide for scientists, students and doctors.
Key Features
- Comprehensive coverage of protein and peptide-based microarrays for multiplex detection
- Cutting-edge guide to the field
- An excellent resource for scientists, students and doctors
Author(s): Navid Rabiee, Michael R. Hamblin
Publisher: IOP Publishing
Year: 2020
Language: English
Pages: 69
City: Bristol
PRELIMS.pdf
Preface
Acknowledgements
Editor biographies
Navid Rabiee
Michael R Hamblin
List of contributors
Outline placeholder
Sakineh Hajebi
Sepideh Ahmadi
Mohammad Rabiee
Mojtaba Bagherzadeh
Saeid Maghsoudi
CH001.pdf
Chapter 1 Introduction to protein microarrays
1.1 Background, history and development
1.2 Protein structure
1.3 Protein microarrays
References
CH002.pdf
Chapter 2 Protein and peptide-based microarrays
2.1 Peptide-based microarrays
2.1.1 Origin and development of peptide arrays
2.1.2 Applications of peptide-based microarrays
2.1.3 Kinase detection
2.1.4 Recognition of proteases and other hydrolytic enzymes
2.1.5 Other applications of peptide arrays
2.2 Protein-based microarrays
2.2.1 Potential need for protein microarrays
2.2.2 Protein therapeutics
2.2.3 Clinical diagnostics
2.2.4 National security and biological threats
2.2.5 Recent applications of protein microarrays
2.3 Problems and challenges
2.4 Sample preparation and handling (probe and target)
2.4.1 Microarray platforms
2.4.2 Detection technologies
2.4.3 Data analysis
2.5 Potential solutions: enabling technologies and advancements
2.5.1 Protein microarray surface chemistry and coupling schemes
2.5.2 Background and recent state of biomolecule libraries
2.5.3 Microarrays based on glass substrates
2.5.4 Surface modification
2.5.5 Current state of glass-based protein microarrays
2.5.6 Microarrays based on gold substrates
2.5.7 Surface modifications
2.5.8 Current state of gold-based protein microarrays
2.5.9 Microarrays on polymer substrates
2.5.10 Surface modifications
2.5.11 Current state of polymer-based microarrays
2.6 Specific formats: microfluidic devices and integrated semiconductor chips
2.7 Site-specific immobilization of antibodies
References
CH003.pdf
Chapter 3 Protein microarrays: materials and fabrication methods
3.1 Materials
3.1.1 Microarrays on polymer substrates
3.1.2 Optical resins
3.1.3 Silicon
3.1.4 Microarrays based on glass substrates
3.1.5 Microarrays based on gold substrates
3.2 Methods
3.2.1 Design
3.2.2 Chemical immobilization techniques for proteins
3.2.3 Covalent chemical coupling
3.2.4 Photochemical cross-linking
3.3 Preparation of protein arrays
3.3.1 Light-directed parallel chemical synthesis
3.3.2 SPOT™ synthesis
3.3.3 Spotting microarray approach
References
CH004.pdf
Chapter 4 Protein microarrays: conclusions and future perspectives
References
