The subject of optogenetics is comprehensively covered in this book, including physical, chemical, and biological topics of light-sensing proteins and their application in biological systems, particularly in neuroscience and medicine and the related opto-electronics. Optogenetics is a new technology that combines genetics and optics. It enables one to manipulate or measure the function of identified cells or neurons in a tissue by light with an accuracy in the range of milliseconds, even in a freely moving animal. Optogenetics has already become a powerful tool for revealing the neural mechanisms underlying behavior and analyzing various physiological phenomena. It is also expected to become useful for treating neural dysfunctions such as Parkinson disease and for the development of a brain–machine interface. This book should be read by any scientist or student performing research in any way related to optogenetics. As a milestone publication on optogenetics, this book will serve as a compass for any researcher, from beginners to experts, to explore this uncharted world.
Author(s): Hiromu Yawo, Hideki Kandori, Amane Koizumi (eds.)
Edition: 1
Publisher: Springer Japan
Year: 2015
Language: English
Pages: 409
Tags: Neurosciences; Cell Biology; Protein Science
Front Matter....Pages i-ix
Front Matter....Pages 1-1
History and Perspectives of Light-Sensing Proteins....Pages 3-16
Biology of Light-Sensing Proteins in Plants and Microorganisms....Pages 17-29
Structure-Functional Analysis of Channelrhodopsins....Pages 31-45
Photochemistry of Halorhodopsin....Pages 47-62
Molecular Mechanisms for Ion Transportation of Microbial Rhodopsins Studied by Light-Induced Difference FTIR Spectroscopy....Pages 63-76
Optogenetic Potentials of Diverse Animal Opsins....Pages 77-88
Color Tuning in Retinylidene Proteins....Pages 89-107
Front Matter....Pages 109-109
General Description: Future Prospects of Optogenetics....Pages 111-131
Optogenetic Manipulation and Probing....Pages 133-147
Probing Neuronal Activity Using Genetically Encoded Red Fluorescent Calcium Indicators....Pages 149-158
Optogenetic Sensors for Monitoring Intracellular Chloride....Pages 159-183
Optogenetic Imaging of Protein Activity in the Synapse Using 2-Photon Fluorescence Lifetime Imaging Microscopy....Pages 185-197
Optogenetics Research Using the Mouse as a Model System....Pages 199-212
Front Matter....Pages 213-226
Neuroscientific Frontline of Optogenetics....Pages 227-237
Elucidation of Neuronal Circuitry Involved in the Regulation of Sleep/Wakefulness Using Optogenetics....Pages 239-239
Optogenetic Analysis of Striatal Connections to Determine Functional Connectomes....Pages 241-248
Potential of Optogenetics for the Behavior Manipulation of Non-human Primates....Pages 249-263
Activity Regulation in the Study of Neural Plasticity....Pages 265-277
Front Matter....Pages 279-290
Strategies to Probe Mechanoreception: From Mechanical to Optogenetic Approaches....Pages 291-304
Casting Light on the Role of Glial Cells in Brain Function....Pages 239-239
Front Matter....Pages 305-314
Towards Understanding the Neural Mechanism of Behavioral Phenotypes Seen in Psychiatric Disorders....Pages 315-327
Establishment of Gene Therapy Using Channelrhodopsin-2 to Treat Blindness....Pages 329-329
Optogenetic Approaches to Restoring Intrinsic Visual Processing Features in Retinal Ganglion Cells....Pages 331-339
On Optogenetic Tissue Engineering on Visual Cells: A Review on Its Development, Practices and Application....Pages 341-352
Front Matter....Pages 353-365
CMOS-Based Neural Interface Device for Optogenetics....Pages 367-372
Use of Channelrhodopsin in the Development of Neural-Network High-Throughput Screening Devices....Pages 373-373
Back Matter....Pages 375-389
....Pages 391-403
