Microbial Biotechnology

Intro
Contents
Preface
List of Contributors
Part I Principles of Microbial Biotechnology
Chapter 1 Microbial Screening Allan Lim and Tan Hai-Meng
1.1. Introduction
1.2. Screening Process
1.3. Screening Methods
1.4. Screening Microorganisms for Probiotic Functions
1.5. Screening Microorganisms for Enzymes
1.5.1. Phytase
1.5.2. Cellulase
1.6. Screening Microorganisms for Metabolites
1.6.1. Primary metabolite: Citric acid
1.6.2. Secondary metabolite: Antibiotics
1.7. Screening Microorganisms for Flavor
References
Further Reading
Websites Chapter 2 Bioprocess Technology Lee Yuan Kun2.1. Microbial Growth
2.1.1. Growth kinetics
2.1.2. Variations in growth kinetics
2.1.3. Effect of growth-limiting substrate concentration
2.1.4. Growth yield
2.2. Product Formation in Microbial Culture
2.3. Culture Medium for Fermentation
2.4. Microbial Cultivation Methods
2.4.1. Closed culture system
2.4.2. Open culture system
Further Reading
Websites
Chapter 3 Application of -Omics Technologies in Microbial Fermentation Victor Wong Vai-Tak, Faraaz Noor Khan Yusufi and Lee Dong-Yup
3.1. Introduction
3.2. Genomics and Transcriptomics 3.2.1. DNA microarray construction3.2.2. Hybridization and scanning
3.2.3. Analysis
3.3. Proteomics
3.3.1. Two-dimensional gel electrophoresis
3.3.2. Isobaric tag for relative and absolute quantitation
3.4. Metabolomics
3.4.1. Types of metabolome analysis
3.4.2. Sample preparation
3.4.3. Detection methods
3.5. Bioinformatics
3.5.1. Data preprocessing and normalization
3.5.2. Data analysis
Further Reading
Chapter 4 Enzymology Theresa Tan May Chin and Deng Lih Wen
4.1. Enzymes as Catalysts
4.2. Nomenclature
4.3. Cofactors
4.4. Regulation of Enzyme Activity 4.5. The Michaelis-Menten Equation4.6. Inhibition of Enzyme Activity
4.7. Industrial Applications of Enzymes
References
Further Reading
Website
Chapter 5 Microbial Genome Mining for Identifying Antimicrobial Targets Kishore R. Sakharkar, Jayaraman Premkumar, Meena K. Sakharkar and Vincent T. K. Chow
5.1. Introduction
5.2. What are Targets?
5.3. Approaches for Genome Mining for Identifying Targets
5.3.1. Essential genes
5.3.2. Selectivity
5.3.3. Structural genomics
5.3.4. Drug target identification and validation in Pseudomonas aeruginosa 5.4. Genomics and Target-Based Antibacterial Discovery5.5. Microbial Genome Mining and Vaccine Design
5.5.1. Reverse vaccinology
5.6. Conclusions and Future Prospects
Further Reading
Chapter 6 Manipulation of Genes Azlinda Anwar, Too Heng-Phon and Chua Kim Lee
6.1. Construction of Recombinant DNA
6.1.1. Enzymes used in gene manipulations
Restriction Endonuclease
6.1.2. Use of restriction endonucleases in recombinant DNA technology
6.1.3. Analysis of the result of restriction endonuclease digestion
6.1.4. DNA ligase and its use in recombinant DNA technology
6.2. Host Organisms