This book highlights the current state of the art in magnetophoretic circuits and their use in the emerging field of single-cell analysis. This interdisciplinary topic involves many fields of science including cellular biology, drug screening, cancer research, personalized medicine, microfabrication, biomedical microdevices, and lab-on-a-chip. This book not only provides the required fundamental knowledge and background needed in magnetics and the circuit theory but also describes the idea of magnetophoretic circuits as well as the cutting-edge developed technologies. It provides a sufficient background in i) the required theory in magnetics, ii) SCAs in general, iii) the circuit theory, iv) the developed idea of the magnetophoretic circuits, v) the fabrication process and magnetic cell labeling techniques, vi) the magnetophoretic-based SCA tools, and vii) the bio-applications. Methods for performing simulations as well as designing, fabricating, and running experiments are explained. Author of the book is one of the inventors of some of the ideas and the author/co-author of some of the related articles in high-impact journals. The book appeals to the readers interested in clinical applications as well as the ones interested in its technical aspects. It is beneficial for researchers interested in the field of single-cell analysis from various disciplines including biomedical engineering, mechanical engineering, electrical engineering, materials science, and cellular biology.
Author(s): Roozbeh Abedini-Nassab
Publisher: Springer
Year: 2023
Language: English
Pages: 185
City: Singapore
Preface
Contents
Symbols
List of Tables
1 Introduction
1.1 Particle and Droplet Manipulation Techniques
1.1.1 Flow-Based Systems
1.1.2 Array-Based Systems
1.2 Applications of Magnetic Techniques in Biology
1.2.1 In Vivo Applications
1.2.2 In Vitro Applications
References
2 Circuit Theory
2.1 Electrical and Electronic Circuits
2.2 Optical Circuits
2.3 Magnetophoretic Circuits
References
3 Theory and Simulation Methods
3.1 Magnetic Materials
3.2 Analytical Modeling
3.2.1 Force on Magnetic Particles
3.2.2 Magnetic Field Sources
3.3 Computational Methods
3.3.1 Semi-Analytical Solution
3.3.2 Hybrid (Finite-Element-Method/Analytical) Solution
3.4 Particle Trajectory Analysis
References
4 Experimental Methods
4.1 Microfabrication
4.1.1 Photolithography
4.1.2 Metallic Thin Film Deposition
4.1.3 Lift-Off
4.1.4 Insulating Thin Film Deposition
4.1.5 Soft Lithography
4.1.6 Reactive Ion Etching
4.2 Surface Functionalization
4.2.1 SiO2 Surface Passivation
4.2.2 SU8 Surface Passivation
4.2.3 Surface Functionalization for Biocompatibility Purposes
4.3 Packing Methods
4.3.1 3D Printing
4.3.2 Electrical Connections
4.4 Rotating Magnetic Field Generator
4.5 Monitoring System
4.6 Image Processing
4.7 Cell Magnetic Labeling
References
5 Magnetophoretic Circuits Operating in an In-Plane Magnetic Field
5.1 Conductors
5.2 Diodes
5.3 Capacitors
5.4 Transistors
5.5 Bends
References
6 Magnetophoretic Circuits Operating in a Tri-Axial Magnetic Field
6.1 Conductors
6.2 Diodes
6.3 Transistors
6.3.1 Attractive Transistors
6.3.2 Repulsive Transistors
6.4 Bends
References
7 Magnetomicrofluidic Circuits
7.1 Magnetophoretic Random Access Memory
7.2 Magnetomicrofluidics
7.3 Silicon-Glass-Based Magnetomicrofluidics
7.3.1 Microchannel Surface Passivation
7.3.2 Silicon-Glass-Based Magnetomicrofluidic Fabrication Protocol
7.3.3 Assembling and Interfacing
7.3.4 Magnetomicrofluidic Circuit Operation
7.4 Biocompatibility
7.4.1 Shear Stress Effects
7.4.2 Magnetic Labeling Effects
7.4.3 Microenvironment Effects
7.5 Applications
7.6 Conclusions
References
