This second edition volume expands on the previous edition with new sections describing the characterization of peptides bound to major histocompatibility complexes (MHC) on the surface of the cell. Chapters also cover topics such as using SERPA for antigen identification; antigen content of electroimmunoprecipitates; whole genome-phage display libraries; antigens in immune complexes; and immunoproteomic biomarkers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Immunoprotemics: Methods and Protocols, Second Edition is a valuable resource that presents novice and expert researchers with techniques that are easily transferrable to laboratories and provides enhanced hands-on insights into this evolving field.
Author(s): Kelly M. Fulton (ed.), Susan M. Twine (ed.)
Series: Methods in Molecular Biology 2024
Edition: 2
Publisher: Springer
Year: 2019
Language: English
Pages: 380
City: NY
Tags: Methods in Molecular Biology
Front Matter ....Pages i-xi
Introduction to the Immune System (Scott McComb, Aude Thiriot, Bassel Akache, Lakshmi Krishnan, Felicity Stark)....Pages 1-24
Immunoproteomics Methods and Techniques (Kelly M. Fulton, Isabel Baltat, Susan M. Twine)....Pages 25-58
Classical Immunoproteomics: Serological Proteome Analysis (SERPA) for Antigen Identification (Kelly M. Fulton, Anna Ananchenko, Lawrence Wolfraim, Shannon Martin, Susan M. Twine)....Pages 59-78
Profiling of Cytokine and Chemokine Responses Using Multiplex Bead Array Technology (Greg Harris, Wangxue Chen)....Pages 79-94
Preparation of the Low Molecular Weight Serum Proteome for Mass Spectrometry Analysis (Timothy D. Veenstra)....Pages 95-102
Enriching for Low-Abundance Serum Proteins Using ProteoMiner™ and Protein-Level HPLC (Sophie Moggridge, Kelly M. Fulton, Susan M. Twine)....Pages 103-117
Identification of the Antigen Content of Electroimmunoprecipitates (N. Helena Beyer, Niels H. H. Heegaard)....Pages 119-135
Detection of Human Norovirus-Specific Antibodies Using the Luciferase Immunoprecipitation System (LIPS) Assay (Christine M. Tin, Stanislav V. Sosnovtsev)....Pages 137-152
Intact Mass Spectrometry Analysis of Immuno-Isolated Human Therapeutic Antibodies from Serum (Christie E. Delaney, John F. Kelly, Wen Ding, Arsalan S. Haqqani)....Pages 153-166
Antigen Identification for Cell-Binding Antibodies Using Ligand-Directed Crosslinking and Biotin Transfer (Tammy-Lynn Tremblay, Jennifer J. Hill)....Pages 167-180
Whole-Genome Phage Display Libraries: A Powerful Tool for Antigen Discovery (Elisa Beghetto, Nicola Gargano)....Pages 181-198
Multiplexed Detection of Autoantibodies to Glycopeptides Using Microarray (Johannes W. Pedersen, Alexander Nøstdal, Hans H. Wandall)....Pages 199-211
Construction and Screening of an Antigen-Derived Peptide Library Displayed on Yeast Cell Surface for CD4+ T Cell Epitope Identification (Fei Wen, Mason R. Smith, Huimin Zhao)....Pages 213-234
Isolation of Major Histocompatibility Complex (MHC)-Associated Peptides by Immunoaffinity Purification (Sarah A. Penny, Stacy A. Malaker)....Pages 235-243
Enhanced Mass Spectrometry Detection of MHC Peptides (Rui Chen, Jianjun Li)....Pages 245-257
Enrichment of Phosphorylated MHC Peptides with Immobilized Metal Affinity Chromatography and Titanium Dioxide Particles (Rui Chen, Jianjun Li)....Pages 259-268
Mass Spectrometric Identification and Molecular Modeling of Glycopeptides Presented by MHC Class I and II Processing Pathways (Stacy A. Malaker, Michael J. Ferracane)....Pages 269-285
Genome-Based Bioinformatic Prediction of Major Histocompatibility (MHC) (Simon J. Foote)....Pages 287-300
Enhancing Mass Spectrometry-Based MHC-I Peptide Identification Through a Targeted Database Search Approach (Prathyusha Konda, J. Patrick Murphy, Morten Nielsen, Shashi Gujar)....Pages 301-307
Prioritization of Therapeutic Targets of Inflammation Using Proteomics, Bioinformatics, and In Silico Cell-Cell Interactomics (Arsalan S. Haqqani, Danica B. Stanimirovic)....Pages 309-325
Genomics-Driven Immunoproteomics: An Integrative Platform to Uncover Important Biomarkers for Human Diseases (Raghavendra Giri, Veneta Qendro, Pooja Rani, Carren Jepchumba, Grace Bugos, Volker Stadler et al.)....Pages 327-332
Identification of Antigens in Immune Complexes (Nozomi Aibara, Kaname Ohyama)....Pages 333-338
Analysis and Characterization of Immune Cells and Their Activation Status by Whole-Cell MALDI-TOF Mass Spectrometry (Richard Ouedraogo, Julien Textoris, Laurent Gorvel, Aurélie Daumas, Christian Capo, Jean-Louis Mege)....Pages 339-351
A Molecular Immunoproteomics Approach to Assess the Viral Antigenicity of Influenza (Kevin M. Downard)....Pages 353-369
Immunoproteomic Biomarkers: From Publication to Personalized Medicine (Devanand M. Pinto)....Pages 371-377
Back Matter ....Pages 379-380
