Histopathology Atlas of Acute Radiation Syndrome and Delayed Effects in Rhesus Macaques: Kidney, Lung, Heart, Intestine and Mesenteric Lymph Node provides a thoroughly illustrated review of the tissue damage and reparative changes associated with standardized irradiation doses in rhesus macaques. In addition to time-sequenced, routinely stained histologic sections, the book presents results of numerous histochemical, immunohistochemical and chromogenic in-situ stains that provide insights into the pathogenesis of radiation-associated tissue injury and repair. This book is compiled and written by a board-certified veterinary pathologist with more than 40 years of experience in the interpretation of experimentally induced tissue alterations in laboratory animals.
This is an important resource for researchers in the field of animal science radiation injury, including radiation oncologists and individuals involved in disaster preparedness related to accidental or deliberate radiation exposure in large populations.
Author(s): George Parker
Publisher: Academic Press
Year: 2022
Language: English
Pages: 292
City: London
Front Cover
Histopathology Atlas of Acute Radiation Syndrome and Delayed Effects in Rhesus Macaques
Copyright Page
Contents
Foreword
Preface
Overview of studies
Irradiation protocol
Animal cage-side observations
Medical management
Dexamethasone treatment, nonsedated respiratory rate
Euthanasia
Necropsy and histology procedures
Acknowledgments
Funding
References
Further reading
1 Radiation-associated changes in the kidney of rhesus macaques
Overview
Staining and figures list
Detailed microscopic observations
Hyaline casts
Glomerular congestion
Glomerular thrombosis
Segmental glomerular capillary dilatation
Increased mesangial matrix
Inflammatory cell infiltration
Miscellaneous changes observed in H&E-stained sections
Interstitial fibrosis—trichrome stain
Glomerular fibrosis—trichrome stain
PAS+ mesangial deposits
Phosphotungstic acid-hematoxylin stain
Perls’ iron stain
Toluidine blue stain
Tryptase immunohistochemical stain
Alpha smooth muscle actin immunohistochemical stain
von Willebrand factor immunohistochemical stain
CD31 immunohistochemical stain
Collagen 1 immunohistochemical stain
Connective tissue growth factor immunohistochemical stain
Transforming growth factor β immunohistochemical stain
IL-22 immunohistochemical stain
IL-22 receptor (IL-22R) immunohistochemical stain
Pro-renin receptor immunohistochemical stain
CD38 immunohistochemical stain
CD68 immunohistochemical stain
CD20 immunohistochemical stain
CD3 immunohistochemical stain
Myeloperoxidase immunohistochemical stain
CD163 immunohistochemical stain
Tryptase immunohistochemical stain
References
2 Radiation-associated changes in the lung of rhesus macaques
Overview
Staining and figures list
Detailed microscopic observations
Alveolar macrophage accumulation
Alveolar edema
Type 2 pneumocyte hyperplasia
Hyperplasia, alveolar–bronchiolar
Lymphocyte infiltration
Neutrophil infiltration
Emphysema
Myxoid cells
Interstitial and pleural fibrosis
Toluidine blue stain
Alpha smooth muscle actin immunohistochemical stain
Tryptase immunohistochemical stain
CD31 immunohistochemical stain
Myeloperoxidase immunohistochemical stain
CD163 immunohistochemical stain
CD206 immunohistochemical stain
CD38 immunohistochemical stain
Collagen 1 immunohistochemical stain
Transforming growth factor β immunohistochemical stain
Connective tissue growth factor immunohistochemical stain
References
3 Radiation-associated changes in the heart of rhesus macaques
Overview
Stains and related figures
Detailed microscopic observations
Toluidine blue stain
Tryptase immunohistochemical stain
Alpha smooth muscle actin immunohistochemical stain
Galectin-3 immunohistochemical staining
Caspase 3 immunohistochemical staining
References
4 Radiation-associated changes in the jejunum of rhesus macaques
Overview
Staining and figures list
Detailed microscopic observations
Mucosal epithelial depletion
Trichrome stain
Periodic acid/Schiff (PAS)/Alcian blue stain
Leukocyte infiltration
Toluidine blue stain
Tryptase immunohistochemical stain
Alpha smooth muscle actin immunohistochemical stain
MxA immunohistochemical stain
Lipopolysaccharide core antigen immunohistochemical stain
Ki-67 immunohistochemical stain
Lgr5 in situ hybridization stain
Myeloperoxidase immunohistochemical stain
CD38 immunohistochemical stain
CD163 immunohistochemical stain
CD3 immunohistochemical stain
CD13 immunohistochemical stain
Bmi-1 immunohistochemical stain
IL22 immunohistochemical stain
IL22R immunohistochemical stain
Collagen 1 immunohistochemical stain
Transforming growth factor β immunohistochemical stain
Connective tissue growth factor immunohistochemical stain
5 Radiation-associated changes in the colon of rhesus macaques
Overview
Staining and figure lists
Detailed colon observations
Leukocyte infiltration
Trichrome stain
PAS/Alcian blue stain
Toluidine blue stain
Tryptase immunohistochemical stain
Alpha smooth muscle actin immunohistochemical stain
MxA immunohistochemical stain
Lipopolysaccharide core antigen immunohistochemical stain
Ki-67 immunohistochemical stain
Myeloperoxidase immunohistochemical stain
CD3 immunohistochemical stain
CD13 immunohistochemical stain
Bmi-1 immunohistochemical stain
IL-22 immunohistochemical stain
IL-22R immunohistochemical stain
Collagen 1 immunohistochemical stain
Transforming growth factor β immunohistochemical stain
Connective tissue growth factor immunohistochemical stain
6 Radiation-associated changes in the mesenteric lymph node of rhesus macaques
Overview
Staining and figures list
Detailed microscopic observations
Routine histologic stain
Trichrome stain
Toluidine blue stain
Tryptase immunohistochemical stain
Alpha-smooth muscle actin immunohistochemical stain
MxA immunohistochemical stain
LPS core immunohistochemical stain
Myeloperoxidase immunohistochemical stain
Collagen 1 immunohistochemical stain
Connective tissue growth factor immunohistochemical stain
Transforming growth factor β immunohistochemical stain
Index
Back Cover
